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Polarization-sensitive optical projection tomography for muscle fiber imaging
Fang, Mengjie1,2; Dong, Di1,2; Zeng, Chaoting3; Liang, Xiao1,2; Yang, Xin1,2; Arranz, Alicia4; Ripoll, Jorge5; Hui, Hui1,2; Tian, Jie1,2; Hui Hui, Jie Tian
AbstractOptical projection tomography (OPT) is a tool used for three-dimensional imaging of millimeter-scale biological samples, with the advantage of exhibiting isotropic resolution typically in the micron range. OPT can be divided into two types: transmission OPT (tOPT) and emission OPT (eOPT). Compared with eOPT, tOPT discriminates different tissues based on their absorption coefficient, either intrinsic or after specific staining. However, it fails to distinguish muscle fibers whose absorption coefficients are similar to surrounding tissues. To circumvent this problem, in this article we demonstrate a polarization sensitive OPT system which improves the detection and 3D imaging of muscle fibers by using polarized light. We also developed image acquisition and processing protocols that, together with the system, enable the clear visualization of muscles. Experimental results show that the muscle fibers of diaphragm and stomach, difficult to be distinguished in regular tOPT, were clearly displayed in our system, proving its potential use. Moreover, polarization sensitive OPT was fused with tOPT to investigate the stomach tissue comprehensively. Future applications of polarization sensitive OPT could be imaging other fiber-like structures such as myocardium or other tissues presenting high optical anisotropy.
WOS HeadingsScience & Technology
Indexed BySCI
Funding OrganizationNational Basic Research Program of China (973 Program)(2011CB707700) ; National Natural Science Foundation of China(81227901 ; Chinese Academy of Sciences(2010Y2GA03 ; Marie Curie Intra-European Fellowship program(IEF-2010-275137) ; EC ; Spanish MINECO project(FIS2013-41802-R) ; 61231004 ; 2013Y1GA0004 ; 81501616 ; 2012T1G0036 ; 81301346 ; 2013T1G0013 ; 81527805) ; YZ201502 ; YZ201457 ; XDB02060010)
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000368150800001
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorHui Hui, Jie Tian
Affiliation1.Chinese Acad Sci, Key Lab Mol Imaging, Inst Automat, Beijing 100190, Peoples R China
2.Beijing Key Lab Mol Imaging, Beijing 100190, Peoples R China
3.Southern Med Univ, Dept Hepatobiliary Surg, Zhujiang Hosp, Guangzhou 510282, Guangdong, Peoples R China
4.CSIC UAM, Ctr Mol Biol Severo Ochoa, Madrid 28049, Spain
5.Univ Carlos III Madrid, Dept Bioengn & Aerosp Engn, Madrid 28911, Spain
Recommended Citation
GB/T 7714
Fang, Mengjie,Dong, Di,Zeng, Chaoting,et al. Polarization-sensitive optical projection tomography for muscle fiber imaging[J]. SCIENTIFIC REPORTS,2016,6:19241.
APA Fang, Mengjie.,Dong, Di.,Zeng, Chaoting.,Liang, Xiao.,Yang, Xin.,...&Hui Hui, Jie Tian.(2016).Polarization-sensitive optical projection tomography for muscle fiber imaging.SCIENTIFIC REPORTS,6,19241.
MLA Fang, Mengjie,et al."Polarization-sensitive optical projection tomography for muscle fiber imaging".SCIENTIFIC REPORTS 6(2016):19241.
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