CASIA OpenIR  > 中国科学院分子影像重点实验室
Vertically scanned laser sheet microscopy
Dong, Di1; Arranz, Alicia2; Zhu, Shouping3; Yang, Yujie1; Shi, Liangliang1; Wang, Jun4; Shen, Chen3; Tian, Jie1; Ripoll, Jorge5,6,7; Jie Tian, Jorge Ripoll
2014-10-01
发表期刊JOURNAL OF BIOMEDICAL OPTICS
卷号19期号:10页码:106001-8
文章类型Article
摘要Laser sheet microscopy is a widely used imaging technique for imaging the three-dimensional distribution of a fluorescence signal in fixed tissue or small organisms. In laser sheet microscopy, the stripe artifacts caused by high absorption or high scattering structures are very common, greatly affecting image quality. To solve this problem, we report here a two-step procedure which consists of continuously acquiring laser sheet images while vertically displacing the sample, and then using the variational stationary noise remover (VSNR) method to further reduce the remaining stripes. Images from a cleared murine colon acquired with a vertical scan are compared with common stitching procedures demonstrating that vertically scanned light sheet microscopy greatly improves the performance of current light sheet microscopy approaches without the need for complex changes to the imaging setup and allows imaging of elongated samples, extending the field of view in the vertical direction. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
关键词Light Sheet Microscopy Selective Plane Illumination Microscopy Stripe Artifact
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1117/1.JBO.19.10.106001
关键词[WOS]PLANE ILLUMINATION MICROSCOPY ; DEVELOPMENTAL BIOLOGY
收录类别SCI
语种英语
项目资助者Bill and Melinda Gates Foundation ; National Basic Research Program of China (973 Program)(2011CB707700) ; National Natural Science Foundation of China(81227901 ; Chinese Academy of Sciences(2010Y2GA03 ; Marie Curie Intra-European Fellowship program(IEF-2010-275137) ; EC FP7 IMI project PREDICT-TB ; EC FP7 CIG grant HIGH-THROUGHPUT TOMO ; Spanish MINECO(FIS2013-41802-R MESO-IMAGING) ; 81027002 ; YZ201164) ; 61231004 ; 81101095)
WOS研究方向Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS类目Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS记录号WOS:000345837200018
引用统计
文献类型期刊论文
条目标识符http://ir.ia.ac.cn/handle/173211/11158
专题中国科学院分子影像重点实验室
通讯作者Jie Tian, Jorge Ripoll
作者单位1.Chinese Acad Sci, Key Lab Mol Imaging, Beijing 100190, Peoples R China
2.ETH, Swiss Fed Inst Technol, Inst Biomed Engn, Zurich, Switzerland
3.Xidian Univ, Sch Life Sci & Technol, Xian 710071, Shaanxi, Peoples R China
4.Harbin Univ Sci & Technol, Sch Automat, Harbin 150080, Peoples R China
5.Univ Carlos III Madrid, Dept Bioengn & Aerosp Engn, Madrid 28911, Spain
6.Hosp Gen Gregorio Maranon, Inst Invest Sanitaria, Expt Med & Surg Unit, Madrid 28007, Spain
7.Fdn Res & Technol Hellas, Inst Elect Struct & Laser, Iraklion 71110, Greece
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GB/T 7714
Dong, Di,Arranz, Alicia,Zhu, Shouping,et al. Vertically scanned laser sheet microscopy[J]. JOURNAL OF BIOMEDICAL OPTICS,2014,19(10):106001-8.
APA Dong, Di.,Arranz, Alicia.,Zhu, Shouping.,Yang, Yujie.,Shi, Liangliang.,...&Jie Tian, Jorge Ripoll.(2014).Vertically scanned laser sheet microscopy.JOURNAL OF BIOMEDICAL OPTICS,19(10),106001-8.
MLA Dong, Di,et al."Vertically scanned laser sheet microscopy".JOURNAL OF BIOMEDICAL OPTICS 19.10(2014):106001-8.
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