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Three-dimensional microscopy and sectional image reconstruction using optical scanning holography
Zhang X(张鑫); Edmund Lam
Source PublicationApplied Optics
2009
Volume48Issue:34Pages:H113
AbstractFast acquisition and high axial resolution are two primary requirements for three-dimensional microscopy. However, they are sometimes conflicting: imaging modalities such as confocal imaging can deliver superior resolution at the expense of sequential acquisition at different axial planes, which is a timeconsuming process. Optical scanning holography (OSH) promises to deliver a good trade-off between these two goals. With just a single scan, we can capture the entire three-dimensional volume in a digital hologram; the data can then be processed to obtain the individual sections. An accurate modeling of the imaging system is key to devising an appropriate image reconstruction algorithm, especially for real data
where random noise and other imaging imperfections must be taken into account. In this paper we demonstrate sectional image reconstruction by applying an inverse imaging sectioning technique to experimental OSH data of biological specimens and visualizing the sections using the OSA Interactive Science Publishing software.


KeywordOsh
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/12001
Collection09年以前成果
Corresponding AuthorEdmund Lam
Recommended Citation
GB/T 7714
Zhang X,Edmund Lam. Three-dimensional microscopy and sectional image reconstruction using optical scanning holography[J]. Applied Optics,2009,48(34):H113.
APA Zhang X,&Edmund Lam.(2009).Three-dimensional microscopy and sectional image reconstruction using optical scanning holography.Applied Optics,48(34),H113.
MLA Zhang X,et al."Three-dimensional microscopy and sectional image reconstruction using optical scanning holography".Applied Optics 48.34(2009):H113.
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