CASIA OpenIR  > 中国科学院分子影像重点实验室
Volumetric chemical imaging by stimulated Raman projection microscopy and tomography
Chen, Xueli1,2,3; Zhang, Chi3; Lin, Peng3; Huang, Kai-Chih3; Liang, Jimin1,2; Tian, Jie1,2,4; Cheng, Ji-Xin3,5
Source PublicationNATURE COMMUNICATIONS
2017-04-24
Volume8
SubtypeArticle
AbstractVolumetric imaging allows global understanding of three-dimensional (3D) complex systems. Light-sheet fluorescence microscopy and optical projection tomography have been reported to image 3D volumes with high resolutions and at high speeds. Such methods, however, usually rely on fluorescent labels for chemical targeting, which could perturb the biological functionality in living systems. We demonstrate Bessel-beam-based stimulated Raman projection (SRP) microscopy and tomography for label-free volumetric chemical imaging. Our SRP microscope enables fast quantitation of chemicals in a 3D volume through a two-dimensional lateral scan. Furthermore, combining SRP and sample rotation, we demonstrate the SRP tomography that can reconstruct the 3D distribution of chemical compositions with optical spatial resolution at a higher speed than the Gaussian-beam-based stimulated Raman scattering sectioning imaging can. We explore the potential of our SRP technology by mapping polymer particles in 3D volumes and lipid droplets in adipose cells.
WOS HeadingsScience & Technology
DOI10.1038/ncomms15117
WOS KeywordLIGHT-SHEET MICROSCOPY ; PLANE ILLUMINATION MICROSCOPY ; SCATTERING MICROSCOPY ; IN-VIVO ; RESOLUTION ; SPECIMENS ; BIOLOGY ; BEAMS
Indexed BySCI
Language英语
Funding OrganizationKeck Foundation ; National Institutes of Health (NIH)(GM118471) ; National Natural Science Foundation of China(81627807 ; China Scholarship Council(201406965025) ; Tianjin University ; 81227901 ; 81571725)
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000400112500001
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/15096
Collection中国科学院分子影像重点实验室
Affiliation1.Xidian Univ, Engn Res Ctr Mol & Neuro Imaging, Minist Educ, Xian 710126, Shaanxi, Peoples R China
2.Xidian Univ, Sch Life Sci & Technol, Xian 710126, Shaanxi, Peoples R China
3.Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
4.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China
5.Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
Recommended Citation
GB/T 7714
Chen, Xueli,Zhang, Chi,Lin, Peng,et al. Volumetric chemical imaging by stimulated Raman projection microscopy and tomography[J]. NATURE COMMUNICATIONS,2017,8.
APA Chen, Xueli.,Zhang, Chi.,Lin, Peng.,Huang, Kai-Chih.,Liang, Jimin.,...&Cheng, Ji-Xin.(2017).Volumetric chemical imaging by stimulated Raman projection microscopy and tomography.NATURE COMMUNICATIONS,8.
MLA Chen, Xueli,et al."Volumetric chemical imaging by stimulated Raman projection microscopy and tomography".NATURE COMMUNICATIONS 8(2017).
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