Volumetric chemical imaging by stimulated Raman projection microscopy and tomography

doi:10.1038/ncomms15117

	Volumetric chemical imaging by stimulated Raman projection microscopy and tomography
	Chen, Xueli 1,2,3; Zhang, Chi 3; Lin, Peng3 ; Huang, Kai-Chih 3; Liang, Jimin 1,2; Tian, Jie 1,2,4; Cheng, Ji-Xin 3,5
发表期刊	NATURE COMMUNICATIONS
	2017-04-24
卷号	8
文章类型	Article
摘要	Volumetric imaging allows global understanding of three-dimensional (3D) complex systems. Light-sheet fluorescence microscopy and optical projection tomography have been reported to image 3D volumes with high resolutions and at high speeds. Such methods, however, usually rely on fluorescent labels for chemical targeting, which could perturb the biological functionality in living systems. We demonstrate Bessel-beam-based stimulated Raman projection (SRP) microscopy and tomography for label-free volumetric chemical imaging. Our SRP microscope enables fast quantitation of chemicals in a 3D volume through a two-dimensional lateral scan. Furthermore, combining SRP and sample rotation, we demonstrate the SRP tomography that can reconstruct the 3D distribution of chemical compositions with optical spatial resolution at a higher speed than the Gaussian-beam-based stimulated Raman scattering sectioning imaging can. We explore the potential of our SRP technology by mapping polymer particles in 3D volumes and lipid droplets in adipose cells.
WOS标题词	Science & Technology
DOI	10.1038/ncomms15117
关键词[WOS]	LIGHT-SHEET MICROSCOPY ; PLANE ILLUMINATION MICROSCOPY ; SCATTERING MICROSCOPY ; IN-VIVO ; RESOLUTION ; SPECIMENS ; BIOLOGY ; BEAMS
收录类别	SCI
语种	英语
项目资助者	Keck Foundation ; National Institutes of Health (NIH)(GM118471) ; National Natural Science Foundation of China(81627807 ; China Scholarship Council(201406965025) ; Tianjin University ; 81227901 ; 81571725)
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000400112500001
引用统计	被引频次：60[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/15096
专题	中国科学院分子影像重点实验室
作者单位	1.Xidian Univ, Engn Res Ctr Mol & Neuro Imaging, Minist Educ, Xian 710126, Shaanxi, Peoples R China 2.Xidian Univ, Sch Life Sci & Technol, Xian 710126, Shaanxi, Peoples R China 3.Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA 4.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China 5.Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
推荐引用方式 GB/T 7714	Chen, Xueli,Zhang, Chi,Lin, Peng,et al. Volumetric chemical imaging by stimulated Raman projection microscopy and tomography[J]. NATURE COMMUNICATIONS,2017,8.
APA	Chen, Xueli.,Zhang, Chi.,Lin, Peng.,Huang, Kai-Chih.,Liang, Jimin.,...&Cheng, Ji-Xin.(2017).Volumetric chemical imaging by stimulated Raman projection microscopy and tomography.NATURE COMMUNICATIONS,8.
MLA	Chen, Xueli,et al."Volumetric chemical imaging by stimulated Raman projection microscopy and tomography".NATURE COMMUNICATIONS 8(2017).