Improved resection and prolonged overall survival with PD-1-IRDye800CW fluorescence probe-guided surgery and PD-1 adjuvant immunotherapy in 4T1 mouse model

doi:10.2147/IJN.S149235

	Improved resection and prolonged overall survival with PD-1-IRDye800CW fluorescence probe-guided surgery and PD-1 adjuvant immunotherapy in 4T1 mouse model
	Du, Yang1,2 ; Sun, Ting 3; Liang, Xiaolong 4; Li, Yuan 3; Jin, Zhengyu 3; Xue, Huadan 3; Wan, Yihong 5; Tian, Jie 1,2
发表期刊	INTERNATIONAL JOURNAL OF NANOMEDICINE
	2017
卷号	12 期号:12 页码:8337-8351
文章类型	Article
摘要	An intraoperative technique to accurately identify microscopic tumor residuals could decrease the risk of positive surgical margins. Several lines of evidence support the expression and immunotherapeutie effect of PD-1 in breast cancer. Here, we sought to develop a fluorescence-labeled PD-1 probe for in vivo breast tumor imaging and image-guided surgery. The efficacy of PD-1 monoclonal antibody (PD-1 mAb) as adjuvant immunotherapy after surgery was also assessed. PD-1-I RDyc800CW was developed and examined for its application in tumor imaging and image-guided tumor resection in an immunocompetent 4T1 mouse tumor model. Fluorescence molecular imaging was performed to monitor probe biodistribution and intraoperative imaging. Bioluminescence imaging was performed to monitor tumor growth and evaluate postsurgical tumor residuals, recurrences, and metastases. The PD-1-IRDye800CW exhibited a specific signal at the tumor region compared with the IgG control. Furthermore, PD-1-IRDye800CW-guided surgery combined with PD-1 adjuvant immunotherapy inhibited tumor rcgrowth and microtumor metastases and thus improved survival rate. Our study demonstrates the feasibility of using PD-1-IRDye800CW for breast tumor imaging and image-guided tumor resection. Moreover, PD-1 mAb adjuvant immunotherapy reduces cancer recurrences and metastases emanating from tumor residuals.
关键词	Pd-1 Programmed Cell Death-1 Near-infrared Fluorescence (Nirf) Breast Cancer Image-guided Surgery Immunotherapy Fluorescence Imaging
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.2147/IJN.S149235
关键词[WOS]	CANCER-IMMUNOTHERAPY ; BREAST-CANCER ; ANTI-PD-L1 ANTIBODY ; ADVANCED MELANOMA ; SAFETY ; RESPONSES ; EXPRESSION ; BLOCKADE ; LYMPHOCYTES ; NIVOLUMAB
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(81227901 ; Research and Development Program of China (973)(2014CB748600 ; Strategic Priority Research Program from Chinese Academy of Sciences(XDB02060010) ; Beijing Natural Science Foundation(Z16110200010000) ; Peking University Third Hospital(BYSY2015023) ; China Postdoctoral Science Foundation(2013M530014) ; Cancer Prevention Research Institute of Texas(RP130145) ; US Department of Defense(W81XWH-13-1-0318) ; Welch Foundation(1-1751) ; Mary Kay Foundation(073.14) ; 81470083 ; 2015CB755500) ; 81527805 ; 81571810 ; 61231004 ; 81771846 ; 81201186)
WOS研究方向	Science & Technology - Other Topics ; Pharmacology & Pharmacy
WOS类目	Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
WOS记录号	WOS:000415814100002
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/19584
专题	中国科学院分子影像重点实验室
作者单位	1.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, 95 Zhongguancun East Rd, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Automat, State Key Lab Management & Control Complex Syst, Beijing, Peoples R China 3.Beijing Union Med Coll Hosp, Dept Radiol, Beijing, Peoples R China 4.Peking Univ, Hosp 3, Dept Ultrasound, Beijing, Peoples R China 5.Univ Texas Southwestern Med Ctr Dallas, Dept Pharmacol, Dallas, TX 75390 USA
第一作者单位	中国科学院自动化研究所
推荐引用方式 GB/T 7714	Du, Yang,Sun, Ting,Liang, Xiaolong,et al. Improved resection and prolonged overall survival with PD-1-IRDye800CW fluorescence probe-guided surgery and PD-1 adjuvant immunotherapy in 4T1 mouse model[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2017,12(12):8337-8351.
APA	Du, Yang.,Sun, Ting.,Liang, Xiaolong.,Li, Yuan.,Jin, Zhengyu.,...&Tian, Jie.(2017).Improved resection and prolonged overall survival with PD-1-IRDye800CW fluorescence probe-guided surgery and PD-1 adjuvant immunotherapy in 4T1 mouse model.INTERNATIONAL JOURNAL OF NANOMEDICINE,12(12),8337-8351.
MLA	Du, Yang,et al."Improved resection and prolonged overall survival with PD-1-IRDye800CW fluorescence probe-guided surgery and PD-1 adjuvant immunotherapy in 4T1 mouse model".INTERNATIONAL JOURNAL OF NANOMEDICINE 12.12(2017):8337-8351.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
IJN-du.pdf（4726KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	浏览下载