CASIA OpenIR  > 中国科学院分子影像重点实验室
Biphasic and dosage-dependent regulation of osteoclastogenesis by β-catenin
Wei W; Zeve D; Suh JM; Wang X; Du Y(杜洋); Zerwekh JE; Dechow PC; Graff JM; Wan Y
Source PublicationMolecular and Cellular Biology
2011-12
Volume31Issue:23Pages:4692
AbstractOsteoclasts are bone-resorbing cells essential for skeletal development, homeostasis, and regeneration. They derive from hematopoietic progenitors in the monocyte/macrophage lineage and differentiate in response to RANKL. However, the precise nature of osteoclast progenitors is a longstanding and important question. Using inducible peroxisome proliferator-activated receptor γ (PPARγ)-tTA TRE-GFP (green fluorescent protein) reporter mice, we show that osteoclast progenitors reside specifically in the PPARγ-expressing hematopoietic bone marrow population and identify the quiescent PPARγ(+) cells as osteoclast progenitors. Importantly, two PPARγ-tTA TRE-Cre-controlled genetic models provide compelling functional evidence. First, Notch activation in PPARγ(+) cells causes high bone mass due to impaired osteoclast precursor proliferation. Second, selective ablation of PPARγ(+) cells by diphtheria toxin also causes high bone mass due to decreased osteoclast numbers. Furthermore, PPARγ(+) cells respond to both pathological and pharmacological resorption-enhancing stimuli. Mechanistically, PPARγ promotes osteoclast progenitors by activating GATA2 transcription. These findings not only identify the long-sought-after osteoclast progenitors but also establish unprecedented tools for their visualization, isolation, characterization, and genetic manipulation.
KeywordPeroxisome Proliferator-activated Receptor  (Ppar)
DOI10.1128/MCB.05979-11
Indexed BySCI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/20493
Collection中国科学院分子影像重点实验室
Recommended Citation
GB/T 7714
Wei W,Zeve D,Suh JM,et al. Biphasic and dosage-dependent regulation of osteoclastogenesis by β-catenin[J]. Molecular and Cellular Biology,2011,31(23):4692.
APA Wei W.,Zeve D.,Suh JM.,Wang X.,Du Y.,...&Wan Y.(2011).Biphasic and dosage-dependent regulation of osteoclastogenesis by β-catenin.Molecular and Cellular Biology,31(23),4692.
MLA Wei W,et al."Biphasic and dosage-dependent regulation of osteoclastogenesis by β-catenin".Molecular and Cellular Biology 31.23(2011):4692.
Files in This Item: Download All
File Name/Size DocType Version Access License
zmb4692.pdf(6967KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wei W]'s Articles
[Zeve D]'s Articles
[Suh JM]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wei W]'s Articles
[Zeve D]'s Articles
[Suh JM]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wei W]'s Articles
[Zeve D]'s Articles
[Suh JM]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: zmb4692.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.