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Effects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation
Du Y (杜洋); Yip H
Source PublicationDifferentiation
AbstractBone morphogenetic proteins (BMPs) are secretory signal molecules that have a variety of regulatory functions during embryonic morphogenesis. BMP2 has been shown to induce differentiation in many cell types, mediated through the activation of its target genes: the inhibitors of differentiation (Id1-3) and key transcription factors. In this study, we investigated the effects of BMP2 on mouse neuroblastoma (Neuro2a) cell differentiation and regulation of the expression of Id1-3 and neural-specific transcription factors. Our results showed that BMP2 stimulation upregulated Id1-3 expression at the early stage of application by involvement of the Smad signaling pathway. BMP2 caused phosphorylation of Smad1/5/8 followed by upregulation of Id1-3. Co-incubation with Noggin, a BMP antagonist, or Smad1 siRNA transfection significantly inhibited phosphorylation of Smad1/5/8 and upregulation of Id protein. Furthermore, our results showed that BMP2-induced differentiation of Neuro2a cells into neurons by downregulating the expression of Id1-3 proteins and upregulating the expression of neural-specific transcriptional factors Dlx2, Brn3a, and NeuroD6. The results suggested that the transient upregulation of Id1-3 expression during the early phase of BMP stimulation may play a role in lineage specification and promote differentiation of neuroblastoma cells towards a neuronal phenotype. Subsequently, a coordinated increase in expression of proneural transcription factors and a decrease in Id1-3 expression may culminate in the transition from proliferation to neurogenesis and the terminal neuronal differentiation of neuroblastoma cells.
KeywordBone Morphogenetic Protein 2 (Bmp2) Id1-3 Differentiation
Indexed BySCI
Document Type期刊论文
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GB/T 7714
Du Y ,Yip H. Effects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation[J]. Differentiation,2010,79(2):84.
APA Du Y ,&Yip H.(2010).Effects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation.Differentiation,79(2),84.
MLA Du Y ,et al."Effects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation".Differentiation 79.2(2010):84.
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