Institutional Repository of Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
Effects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation | |
Du Y (杜洋); Yip H | |
发表期刊 | Differentiation |
2010-02 | |
卷号 | 79期号:2页码:84 |
摘要 | Bone morphogenetic proteins (BMPs) are secretory signal molecules that have a variety of regulatory functions during embryonic morphogenesis. BMP2 has been shown to induce differentiation in many cell types, mediated through the activation of its target genes: the inhibitors of differentiation (Id1-3) and key transcription factors. In this study, we investigated the effects of BMP2 on mouse neuroblastoma (Neuro2a) cell differentiation and regulation of the expression of Id1-3 and neural-specific transcription factors. Our results showed that BMP2 stimulation upregulated Id1-3 expression at the early stage of application by involvement of the Smad signaling pathway. BMP2 caused phosphorylation of Smad1/5/8 followed by upregulation of Id1-3. Co-incubation with Noggin, a BMP antagonist, or Smad1 siRNA transfection significantly inhibited phosphorylation of Smad1/5/8 and upregulation of Id protein. Furthermore, our results showed that BMP2-induced differentiation of Neuro2a cells into neurons by downregulating the expression of Id1-3 proteins and upregulating the expression of neural-specific transcriptional factors Dlx2, Brn3a, and NeuroD6. The results suggested that the transient upregulation of Id1-3 expression during the early phase of BMP stimulation may play a role in lineage specification and promote differentiation of neuroblastoma cells towards a neuronal phenotype. Subsequently, a coordinated increase in expression of proneural transcription factors and a decrease in Id1-3 expression may culminate in the transition from proliferation to neurogenesis and the terminal neuronal differentiation of neuroblastoma cells. |
关键词 | Bone Morphogenetic Protein 2 (Bmp2) Id1-3 Differentiation |
收录类别 | SCI |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ia.ac.cn/handle/173211/20496 |
专题 | 中国科学院分子影像重点实验室 |
推荐引用方式 GB/T 7714 | Du Y ,Yip H. Effects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation[J]. Differentiation,2010,79(2):84. |
APA | Du Y ,&Yip H.(2010).Effects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation.Differentiation,79(2),84. |
MLA | Du Y ,et al."Effects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation".Differentiation 79.2(2010):84. |
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