CASIA OpenIR  > 中国科学院分子影像重点实验室
CD146 coordinates brain endothelial cell-pericyte communication for blood-brain barrier development
Chen, Jianan1,2; Luo, Yongting3; Hui, Hui4; Cai, Tanxi1; Huang, Hongxin5; Yang, Fuquan1; Feng, Jing1; Zhang, Jingjing5; Yan, Xiyun1,2
2017-09-05
发表期刊PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷号114期号:36页码:E7622-E7631
文章类型Article
摘要The blood-brain barrier (BBB) establishes a protective interface between the central neuronal system and peripheral blood circulation and is crucial for homeostasis of the CNS. BBB formation starts when the endothelial cells (ECs) invade the CNS and pericytes are recruited to the nascent vessels during embryogenesis. Despite the essential function of pericyte-EC interaction during BBB development, the molecular mechanisms coordinating the pericyte-EC behavior and communication remain incompletely understood. Here, we report a single cell receptor, CD146, that presents dynamic expression patterns in the cerebrovasculature at the stages of BBB induction and maturation, coordinates the interplay of ECs and pericytes, and orchestrates BBB development spatiotemporally. In mouse brain, CD146 is first expressed in the cerebrovascular ECs of immature capillaries without pericyte coverage; with increased coverage of pericytes, CD146 could only be detected in pericytes, but not in cerebrovascular ECs. Specific deletion of Cd146 in mice ECs resulted in reduced brain endothelial claudin-5 expression and BBB breakdown. By analyzing mice with specific deletion of Cd146 in pericytes, which have defects in pericyte coverage and BBB integrity, we demonstrate that CD146 functions as a coreceptor of PDGF receptor-beta to mediate pericyte recruitment to cerebrovascular ECs. Moreover, we found that the attached pericytes in turn down-regulate endothelial CD146 by secreting TGF-beta 1 to promote further BBB maturation. These results reveal that the dynamic expression of CD146 controls the behavior of ECs and pericytes, thereby coordinating the formation of a mature and stable BBB.
关键词Cd146 Claudin-5 Pdgfr Beta Spatiotemporal Expression Blood-brain Barrier
WOS标题词Science & Technology
DOI10.1073/pnas.1710848114
关键词[WOS]GROWTH-FACTOR ; IN-VIVO ; TUMOR ANGIOGENESIS ; RECEPTOR ; CNS ; IDENTIFICATION ; HEALTH ; ESTABLISHMENT ; MICROVESSELS ; MAINTENANCE
收录类别SCI
语种英语
项目资助者National Basic Research Program of China 973 Programs(2015CB553705 ; National Natural Science Foundation of China(31370824 ; Chinese Academy of Sciences(XDA12020207 ; Guangdong Natural Science Fund for Distinguished Young Scholars, China ; Novo Nordisk-Chinese Academy of Sciences(NNCAS-2015-11) ; 2014CBA02003) ; 81371025 ; XDA12030202) ; 91529306 ; 81371330 ; 91640112 ; 31670185 ; 81527805 ; 81671851 ; 81502547)
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000409182200024
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ia.ac.cn/handle/173211/20718
专题中国科学院分子影像重点实验室
作者单位1.Chinese Acad Sci, Inst Biophys, Key Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
3.China Agr Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100193, Peoples R China
4.Chinese Acad Sci, Inst Automat, Key Lab Mol Imaging, Beijing 100190, Peoples R China
5.Guangdong Med Univ, Affiliated Hosp, Zhanjiang 524001, Peoples R China
推荐引用方式
GB/T 7714
Chen, Jianan,Luo, Yongting,Hui, Hui,et al. CD146 coordinates brain endothelial cell-pericyte communication for blood-brain barrier development[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2017,114(36):E7622-E7631.
APA Chen, Jianan.,Luo, Yongting.,Hui, Hui.,Cai, Tanxi.,Huang, Hongxin.,...&Yan, Xiyun.(2017).CD146 coordinates brain endothelial cell-pericyte communication for blood-brain barrier development.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,114(36),E7622-E7631.
MLA Chen, Jianan,et al."CD146 coordinates brain endothelial cell-pericyte communication for blood-brain barrier development".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114.36(2017):E7622-E7631.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Chen, Jianan]的文章
[Luo, Yongting]的文章
[Hui, Hui]的文章
百度学术
百度学术中相似的文章
[Chen, Jianan]的文章
[Luo, Yongting]的文章
[Hui, Hui]的文章
必应学术
必应学术中相似的文章
[Chen, Jianan]的文章
[Luo, Yongting]的文章
[Hui, Hui]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。