Three-dimensional light field microscope based on a lenslet array
Zhang, Mei; Geng, Zheng; Pei, Renjing; Cao, Xuan; Zhang, Zhaoxing
2017-11-15
发表期刊OPTICS COMMUNICATIONS
期号403页码:133-142
文章类型Article
摘要Most conventional microscopes can only acquire two-dimensional (2D) images. However, there is increasing demand for microscopes capable of acquiring three-dimensional (3D) images for use in biological research. To improve observations of in vivo specimens, we develop a novel one snap-shot 3D light field microscope with high spatial resolution. By adding a lenslet array and focusing optics into the optical train of a conventional microscope, we are able to simultaneously capture seven sub-aperture images from different perspectives in a single shot, and finish 3D surface reconstruction of the specimen. In this paper, we present a functioning prototype of a novel 3D light field microscope using different objectives, and describe its design and image quality. During 3D image reconstruction, we use an optical flow method and back-projection interpolation to improve the resolution of the subimages. The performance of the novel light field microscope is analyzed theoretically and experimentally. Multiple perspective images are obtained using an objective with 20 x /NA 0.4. These images are 1050 x 1050 pixels with a depth of view of 200 mu m. For our microscope, the highest resolvable group of USAF 1951 target is about group 7.5. 3D reconstructions of various specimens are successfully created using these images. This novel microscopy system is suitable for various applications in 3D imaging and optical metrology. (C) 2017 Elsevier B.V. All rights reserved.
关键词Light Field Three-dimensional Microscopy High Resolution 3d Image Acquisition 3d Image Reconstruction
WOS标题词Science & Technology ; Physical Sciences
DOI10.1016/j.optcom.2017.07.026
关键词[WOS]ILLUMINATION MICROSCOPY ; NEURONAL-ACTIVITY
收录类别SCI
语种英语
项目资助者863 Program(2015AA015905) ; National Natural Science Foundation of China (NSFC)(61605240)
WOS研究方向Optics
WOS类目Optics
WOS记录号WOS:000410638900021
引用统计
文献类型期刊论文
条目标识符http://ir.ia.ac.cn/handle/173211/20728
专题复杂系统管理与控制国家重点实验室_先进控制与自动化
作者单位Chinese Acad Sci, Inst Automat, State Key Lab Management & Control Complex Syst, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Mei,Geng, Zheng,Pei, Renjing,et al. Three-dimensional light field microscope based on a lenslet array[J]. OPTICS COMMUNICATIONS,2017(403):133-142.
APA Zhang, Mei,Geng, Zheng,Pei, Renjing,Cao, Xuan,&Zhang, Zhaoxing.(2017).Three-dimensional light field microscope based on a lenslet array.OPTICS COMMUNICATIONS(403),133-142.
MLA Zhang, Mei,et al."Three-dimensional light field microscope based on a lenslet array".OPTICS COMMUNICATIONS .403(2017):133-142.
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