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Human embryonic stem cells-derived endothelial cell therapy facilitates kidney regeneration by stimulating renal resident stem cell proliferation in acute kidney injury
Jia Xiaohua
2013-08
发表期刊Chinese Science Bulletin
卷号58期号:23页码:2820
摘要
; Endothelial cell therapy has been implicated to enhance tissue regeneration and vascularization in ischemic kidney. However, no
published study has yet examined direct effects of endothelial cell treatment in kidney recovery. This study investigated the therapeutic efficacy of endothelial cells in a mouse model with acute kidney injury (AKI). Thus, human embryonic stem cells-derived
endothelial cells (hESC-ECs) labeled with a reporter system encoding a double fusion reporter gene for firefly luciferase (Fluc)
and green fluorescent protein (GFP) were characterized by Fluc imaging and immunofluoresence staining. Cultured hESC-ECs
(1×10
6) were injected into ischemic kidney shortly after AKI. Survival of the transplanted hESC-ECs was monitored in vivo from
day 1 to 14 after endothelial cell transplantation and potential impact of hESC-EC treatment on renal regeneration was assessed
by histological analyses. We report that a substantial level of bioluminescence activity was detected 24 h after hESC-EC injection
followed by a gradual decline from 1 to 14 d. Human ESC-ECs markedly accelerated kidney cell proliferation in response to ischaemia-induced damage, indicated by an elevated number of BrdU
+ cells. Co-expression of Sca-1, a kidney stem cell proliferation marker, and BrdU further suggested that the observed stimulation in renal cell regeneration was, at least in part, due to increased proliferation of renal resident stem cells especially within the medullary cords and arteriole. Differentiation of hESC-ECs
to smooth muscle cells was also observed at an early stage of kidney recovery. In summary, our results suggest that endothelial
cell therapy facilitates kidney recovery by promoting vascularization, trans-differentiation and endogenous renal stem cell proliferation in AKI


关键词Molecular Imaging
文献类型期刊论文
条目标识符http://ir.ia.ac.cn/handle/173211/21793
专题中国科学院分子影像重点实验室
推荐引用方式
GB/T 7714
Jia Xiaohua. Human embryonic stem cells-derived endothelial cell therapy facilitates kidney regeneration by stimulating renal resident stem cell proliferation in acute kidney injury[J]. Chinese Science Bulletin,2013,58(23):2820.
APA Jia Xiaohua.(2013).Human embryonic stem cells-derived endothelial cell therapy facilitates kidney regeneration by stimulating renal resident stem cell proliferation in acute kidney injury.Chinese Science Bulletin,58(23),2820.
MLA Jia Xiaohua."Human embryonic stem cells-derived endothelial cell therapy facilitates kidney regeneration by stimulating renal resident stem cell proliferation in acute kidney injury".Chinese Science Bulletin 58.23(2013):2820.
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