Improved Mesenchymal Stem Cell Survival in Ischemic Heart through Electroacupuncture

	Improved Mesenchymal Stem Cell Survival in Ischemic Heart through Electroacupuncture
	Jia Xiaohua
发表期刊	Chin J Integr Med
	2013-08
卷号	19 期号:8 页码:573
摘要	; Objective: To investigate whether electroacupuncture (EA) can promote cell survival and enhance heart function of mesenchymal stem cells (MSCs) therapy. Methods: MSCs were isolated from bone marrow and expanded in Minimum Essential Medium Alpha (α-MEM). MI was induced in 72 Sprague-Dawley (S-D) rats by ligation of the left anterior descending coronary artery (LAD) for 30 min and reperfusion. MI rats randomly received injection of 1×106 DiI-labeled MSCs alone (n=24, MSC group), or plus electroacupuncture (EA) at Neiguan (PC6, n=24, EA+MSC group), or saline (n=24, saline group). EA treatment was performed for 4 days. Another 24 rats were subjected to chest-open surgery without LAD occlusion and treatment (sham group). Three time points, 4, 14 and 28 days (n=8 for each group) were included in this study. The survival of transplanted MSCs and the protective gene expression were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot at day 4 and 14. Left ventricular remodeling, cardiac function, infarction area, fibrosis and capillary density were analyzed at day 28. Results: EA can enhance MSC survival (2.6-fold up) at day 4. Big capillary density was 53% higher in EA+MSC treated group than MSC alone group. Furthermore, the rats treated by EA reduced the fibrosis and had 36% smaller infarct size comparing to MSC alone. EA also attenuated left ventricular remodeling and enhanced the functional recovery of infarcted hearts at week 4. Conclusion: EA at Neiguan acupoint can promote the stem cell survival and improve ischemic heart function. EA could become a useful approach in stem cell therapy for ischemia heart diseases.
关键词	Electroacupuncture
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/21794
专题	中国科学院分子影像重点实验室
推荐引用方式 GB/T 7714	Jia Xiaohua. Improved Mesenchymal Stem Cell Survival in Ischemic Heart through Electroacupuncture[J]. Chin J Integr Med,2013,19(8):573.
APA	Jia Xiaohua.(2013).Improved Mesenchymal Stem Cell Survival in Ischemic Heart through Electroacupuncture.Chin J Integr Med,19(8),573.
MLA	Jia Xiaohua."Improved Mesenchymal Stem Cell Survival in Ischemic Heart through Electroacupuncture".Chin J Integr Med 19.8(2013):573.

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