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Phage Display-Derived Peptide-based Dual-Modality Imaging Probe for Bladder Cancer Diagnosis and Resection Post-Instillation: A Preclinical Study
Li Peng1,2,4; Wenting Shang2,3; Pengyu Guo1,2,4; Kunshan He2,3; Hongzhi Wang1,4; Ziyu Han2,3; Hongmei Jiang2,3; Jie Tian2,3,5; Kun Wang2,3; Wanhai Xu1,4
Source PublicationMolecular Cancer Therapeutics
2018-10
Issue17Pages:2100-2111
AbstractBladder cancer (BC) is a common human malignancy. Conventional ultrasound and
white light cystoscopy are often used for BC diagnosis and resection, but insufficient
specificity results in a high BC recurrence rate. New strategy for the diagnosis and
resection of BC are needed. In this study, we developed a highly specific
peptide-based probe for BC photoacoustic imaging (PAI) diagnosis and near-infrared
(NIR)-imaging-guided resection post-instillation. A BC-specific peptide (PLSWT7)
was selected by
in vivo phage display technology and labeled with IRDye800CW to
synthesize a BC-specific dual-modality imaging (DMI) probe (PLSWT7-DMI). The
feasibility of PLSWT7-DMI-based dual-modality PAI-NIR imaging was assessed
in
vitro
, in mouse models, and ex vivo human bladders. An air-pouch BC (APBC) model
suitable for probe instillation was established to evaluate the probe-based BC PAI
diagnosis and NIR-imaging-guided resection. Human bladders were used to assess
whether PLSWT7-DMI-based DMI strategy is a translatable approach for BC
detection and resection. The probe exhibited excellent selectivity and specificity both

in vitro and in vivo. Post-instillation of the probe, tumors <3 mm were detectable by
PAI, and NIR-imaging-guided tumor resection decreased the BC recurrence rate by
90% and increased the survival in the mouse model. Additionally,
ex vivo NIR
imaging of human bladders indicated that PLSWT7-DMI-based imaging would
potentially allowed precise resection of BC in clinical settings. This
PLSWT7-DMI-based DMI strategy was a translatable approach for BC diagnosis and
resection and could potentially lower the BC recurrence rate.

KeywordBladder Cancer Diagnosis And Resection Preclinical Study
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/22026
Collection中国科学院分子影像重点实验室
Affiliation1.Urology Surgery Department, the Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P. R. China.
2.CAS Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, P. R. China.
3.The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.
4.Heilongjiang Key Laboratory of Scientific Research in Urology, Harbin, Heilongjiang 150001, P. R. China.
5.Beihang University, Beijing 100190, P. R. China
Recommended Citation
GB/T 7714
Li Peng,Wenting Shang,Pengyu Guo,et al. Phage Display-Derived Peptide-based Dual-Modality Imaging Probe for Bladder Cancer Diagnosis and Resection Post-Instillation: A Preclinical Study[J]. Molecular Cancer Therapeutics,2018(17):2100-2111.
APA Li Peng.,Wenting Shang.,Pengyu Guo.,Kunshan He.,Hongzhi Wang.,...&Wanhai Xu.(2018).Phage Display-Derived Peptide-based Dual-Modality Imaging Probe for Bladder Cancer Diagnosis and Resection Post-Instillation: A Preclinical Study.Molecular Cancer Therapeutics(17),2100-2111.
MLA Li Peng,et al."Phage Display-Derived Peptide-based Dual-Modality Imaging Probe for Bladder Cancer Diagnosis and Resection Post-Instillation: A Preclinical Study".Molecular Cancer Therapeutics .17(2018):2100-2111.
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