|The diagnosis of hepatic fibrosis by magnetic resonance and near-infrared imaging using dualmodality nanoparticles|
|Yunfang Li1; Wenting Shang2; Xiaoyuan Liang2; Chaoting Zeng2; Mingming Liu1; Sudan Wang1; Hongjun Li1; Jie Tian2|
|摘要||Hepatic fibrosis (HF), as the only reversible process of chronic liver disease, remains a big diagnostic challenge. Development of noninvasive and effective methods to assess quantitatively early-stage HF is of great clinical importance. Compared with conventional diagnostic methods, near-infrared fluorescence imaging (NIR) and magnetic resonance imaging (MRI) could offer highly sensitive and spatial resolution signals for HF detection.|
However, precise detection using contrast agents is not possible. Superparamagnetic iron oxide (SPIO) nanoparticles have low toxicity, high sensitivity and excellent biocompatibility. Integration of Fe3O4 nanoparticles and indocyanine green (ICG), coupled with targeting ligand of integrin avb3, arginine– glycine–aspartic acid (RGD) expressed on hepatic stellate cells (HSCs), were used to detect HF. Both in vivo and in vitro results showed that the SPIO@SiO2–ICG–RGD had high stability and low cytotoxicity. The
biodistribution of SPIO@SiO2–ICG–RGD was significantly different between mice with HF and healthy controls. SPIO@SiO2–ICG–RGD was characterized and the results of imaging in vitro and in vivo demonstrated the expression of integrin avb3 on activated HSCs. These data suggest that our SPIO@SiO2– ICG–RGD probe could be used for the diagnosis of early-stage HF. This new nanoprobe with a dualmodality imaging approach holds great potential for the diagnosis and classification of HF.
|关键词||Hepatic Fibrosis Spio@sio2– Icg–rgd Diagnosis|
|作者单位||1.Department of Radiology, Beijing YouAn Hospital, Capital Medical University, 8 Xitoutiao, Youanmen, Fengtai District, Beijing, China, 100069|
2.Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Zhongguancun East Road #95, Haidian Dist, Beijing 100190, P. R. China.
|Yunfang Li,Wenting Shang,Xiaoyuan Liang,et al. The diagnosis of hepatic fibrosis by magnetic resonance and near-infrared imaging using dualmodality nanoparticles[J]. RSC Advances,2018(8):6699.|
|APA||Yunfang Li.,Wenting Shang.,Xiaoyuan Liang.,Chaoting Zeng.,Mingming Liu.,...&Jie Tian.(2018).The diagnosis of hepatic fibrosis by magnetic resonance and near-infrared imaging using dualmodality nanoparticles.RSC Advances(8),6699.|
|MLA||Yunfang Li,et al."The diagnosis of hepatic fibrosis by magnetic resonance and near-infrared imaging using dualmodality nanoparticles".RSC Advances .8(2018):6699.|
|13.Yunfang Li.pdf（1495KB）||期刊论文||作者接受稿||开放获取||CC BY-NC-SA||浏览 下载|