CASIA OpenIR
Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging
Li, Tianmeng1,2,3; Hui, Hui2,3,4; Hu, Chaoen2,3; Ma, He1; Yang, Xin2,3; Tian, Jie2,3,4
Source PublicationJOURNAL OF BIOMEDICAL OPTICS
ISSN1083-3668
2019
Volume24Issue:1Pages:8
Corresponding AuthorMa, He(mahe@bmie.neu.edu.cn) ; Yang, Xin(xin.yang@ia.ac.cn) ; Tian, Jie(jie.tian@ia.ac.cn)
AbstractThe objective of our study is to develop a multimodality approach by combining magnetic resonance imaging (MRI) and optical imaging methods to assess acute murine colitis at the macro- and microscopic level. In vivo MRI is used to measure the cross-sectional areas of colons at the macroscopic level. Dual-color confocal laser endomicroscopy (CLE) allows in vivo examination of the fluorescently labeled epithelial cells and micro-vessels in the mucosa with a spatial resolution of similar to 1.4 mu m during ongoing endoscopy. To further validate the structural changes of the colons in three-dimensions, ex vivo light-sheet fluorescence microscopy (LSFM) is applied for in-toto imaging of cleared colon sections. MRI, LSFM, and CLE findings are significantly correlated with histological scoring (p < 0.01) and the inflammation-associated activity index (p < 0.01). Our multimodality imaging technique permits visualization of mucosa in colitis at different scales, which can enhance our understanding of the pathogenesis of inflammatory bowel diseases. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.
Keywordmultimodality imaging confocal laser endomicroscopy light-sheet fluorescence microscopy magnetic resonance imaging inflammatory bowel disease
DOI10.1117/1.JBO.24.1.016003
WOS KeywordINFLAMMATORY-BOWEL-DISEASE ; MOUSE MODEL ; COLONOSCOPY ; MUCOSA ; CANCER
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2016YFC0103803] ; National Key Research and Development Program of China[2017YFA0700401] ; National Key Research and Development Program of China[2017YFA0205200] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[81671851] ; National Natural Science Foundation of China[81227901] ; Strategic Priority Research Program[XDBS01030200] ; Scientific Instrument RD Programs[GJJSTD20170004] ; Scientific Instrument RD Programs[YJKYYQ20170075] ; Frontier Science Key Research Program from the Chinese Academy of Sciences[QYZDJ-SSW-JSC005] ; Beijing Municipal Science and Technology Commission[Z161100002616022]
Funding OrganizationNational Key Research and Development Program of China ; National Natural Science Foundation of China ; Strategic Priority Research Program ; Scientific Instrument RD Programs ; Frontier Science Key Research Program from the Chinese Academy of Sciences ; Beijing Municipal Science and Technology Commission
WOS Research AreaBiochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS SubjectBiochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS IDWOS:000463884000008
PublisherSPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/24929
Collection中国科学院自动化研究所
Corresponding AuthorMa, He; Yang, Xin; Tian, Jie
Affiliation1.Northeastern Univ, Sino Dutch Biomed & Informat Engn Sch, Shenyang, Liaoning, Peoples R China
2.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing, Peoples R China
3.Inst Automat, Beijing Key Lab Mol Imaging, Beijing, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationInstitute of Automation, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Automation, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Tianmeng,Hui, Hui,Hu, Chaoen,et al. Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging[J]. JOURNAL OF BIOMEDICAL OPTICS,2019,24(1):8.
APA Li, Tianmeng,Hui, Hui,Hu, Chaoen,Ma, He,Yang, Xin,&Tian, Jie.(2019).Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging.JOURNAL OF BIOMEDICAL OPTICS,24(1),8.
MLA Li, Tianmeng,et al."Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging".JOURNAL OF BIOMEDICAL OPTICS 24.1(2019):8.
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