CASIA OpenIR  > 中国科学院分子影像重点实验室
In vivo three-dimensional evaluation of tumor hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT
Wenhui Huang; Wang K(王坤); Yu An; Hui Meng; Yuan Gao; Zhiyuan Xiong; Hao Yan; Qian Wang; Xuekang Cai; Xin Yang; Bin Zhang; Qiuying Chen; Xing Yang; Jie Tian; Shuixing Zhang
Source PublicationEuropean Journal of Nuclear Medicine and Molecular Imaging
2019
Volume0Issue:0Pages:0
Contribution Rank1
Abstract

Purpose Accurate evaluation of hypoxia is particularly important in patients with nasopharyngeal carcinoma (NPC) undergoing radiotherapy, yet no clinically accepted methodology for imaging hypoxia is available. The aim of this study was to develop a novel imaging strategy for quantitative three-dimensional (3D) evaluation of hypoxia in a small animal model of NPC.

Methods A carbonic anhydrase IX (CAIX)-specific molecular probe (CAIX-800) was developed for imaging of hypoxia. Mouse models of subcutaneous, orthotopic, and spontaneous lymph node metastasis from NPC (5 mice per group) were established to assess the imaging strategy. A multi-modality imaging method that consisted of a hybrid combination of fluorescence molecular tomography-computed tomography (FMT-CT) and multispectral optoacoustic tomography (MSOT) was used for 3D quantitative evaluation of tumour hypoxia. Magnetic resonance imaging, histological examination, and immunohistochemical analysis were used as references for comparison and validation.

Results In the early stage of NPC (2 weeks after implantation), FMT-CT enabled precise 3D localization of the hypoxia biomarker with high contrast. At the advanced stage (6 weeks after implantation), MSOT allowed multispectral analysis of the biomarker and haemoglobin molecules with high resolution. The combination of high sensitivity and high resolution from FMT-CT and MSOT could not only detect hypoxia in small-sized NPCs but also visualise the heterogeneity of hypoxia in 3D.

Conclusions Integration of FMT-CT and MSOT could allow comprehensive and quantifiable evaluation of hypoxia in NPC. These findings may potentially benefit patients with NPC undergoing radiotherapy in the future.

KeywordNasopharyngeal Carcinoma Tumour Hypoxia Fluorescence Molecular Tomography Multispectral Optoacoustic Tomography
DOI10.1007/s00259-019-04526-x
Indexed BySCI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/25838
Collection中国科学院分子影像重点实验室
Corresponding AuthorXing Yang; Jie Tian; Shuixing Zhang
Recommended Citation
GB/T 7714
Wenhui Huang,Wang K,Yu An,et al. In vivo three-dimensional evaluation of tumor hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT[J]. European Journal of Nuclear Medicine and Molecular Imaging,2019,0(0):0.
APA Wenhui Huang.,Wang K.,Yu An.,Hui Meng.,Yuan Gao.,...&Shuixing Zhang.(2019).In vivo three-dimensional evaluation of tumor hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT.European Journal of Nuclear Medicine and Molecular Imaging,0(0),0.
MLA Wenhui Huang,et al."In vivo three-dimensional evaluation of tumor hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT".European Journal of Nuclear Medicine and Molecular Imaging 0.0(2019):0.
Files in This Item: Download All
File Name/Size DocType Version Access License
EJNM-D-19-00421(1).p(2830KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wenhui Huang]'s Articles
[Wang K(王坤)]'s Articles
[Yu An]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wenhui Huang]'s Articles
[Wang K(王坤)]'s Articles
[Yu An]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wenhui Huang]'s Articles
[Wang K(王坤)]'s Articles
[Yu An]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: EJNM-D-19-00421(1).pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.