CASIA OpenIR
Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects
Zheng, Haifeng1,2; Chen, Si1; Wang, Xinan1; Xie, Jinrong1; Tian, Jie1,3,4; Wang, Fu1,2
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
2019-10-01
Volume91Issue:19Pages:12392-12398
Corresponding AuthorTian, Jie(jie.tian@ia.ac.cn) ; Wang, Fu(fwang@xidian.edu.cn)
AbstractPre-mRNA splicing in the information exchange from DNA to protein is a critical step in all eukaryotes. However, there is currently a lack of noninvasive approaches for monitoring mRNA splicing events in cells. In this study, we presented a genetically encoded bioluminescence reporter, Rluc-intron, for noninvasive real-time monitoring the premRNA splicing process in living cells and animals. It was designed by inserting a renilla luciferase (Rluc) gene into an intron sequence manipulated by RNA splicing modulator. We demonstrated that the splicing reporter Rluc-intron could provide real-time and quantitative information on the splicing activity responded to extracellular stimuli in living cells. In addition, Rluc-intron reporter is able to successfully quantify and image the pre-mRNA splicing in living mice in a noninvasive and longitudinal manner. This bioluminescence reporter provides the advantageous properties of systematic discovery of splicing modulators, which give the advances in pharmacogenomics and would produce new approach in the therapy of human diseases caused by splicing disorder.
DOI10.1021/acs.analchem.9b02935
WOS KeywordANTISENSE OLIGONUCLEOTIDES ; RETENTION
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[81571721] ; National Natural Science Foundation of China[81772010] ; National Key Research and Development Program of China (973 Program)[2017YFA0205202]
Funding OrganizationNational Natural Science Foundation of China ; National Key Research and Development Program of China (973 Program)
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000488993000036
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/26445
Collection中国科学院自动化研究所
Corresponding AuthorTian, Jie; Wang, Fu
Affiliation1.Xidian Univ, Sch Life Sci & Technol, Minist Educ, Engn Res Ctr Mol & Neuro Imaging, Xian 710071, Shaanxi, Peoples R China
2.Northwestern Polytech Univ, Inst Med Res, Xian 710072, Shaanxi, Peoples R China
3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
4.Beihang Univ, Sch Med, Beijing Adv Innovat Ctr Big Data Based Precis Med, Beijing 100190, Peoples R China
Corresponding Author AffilicationChinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Haifeng,Chen, Si,Wang, Xinan,et al. Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects[J]. ANALYTICAL CHEMISTRY,2019,91(19):12392-12398.
APA Zheng, Haifeng,Chen, Si,Wang, Xinan,Xie, Jinrong,Tian, Jie,&Wang, Fu.(2019).Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects.ANALYTICAL CHEMISTRY,91(19),12392-12398.
MLA Zheng, Haifeng,et al."Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects".ANALYTICAL CHEMISTRY 91.19(2019):12392-12398.
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