A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer

doi:10.1016/j.biomaterials.2018.08.048

	A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer
	Wang, Qian 1,2,3; Yan, Hao 3,4; Jin, Yushen3 ; Wang, Zihua 5; Huang, Wenhui 3; Qiu, Jia 3; Kang, Feiyu 4; Wang, Kun3 ; Zhao, Xinming 1,2; Tian, Jie 3
发表期刊	BIOMATERIALS
ISSN	0142-9612
	2018-11-01
卷号	183 页码:173-184
通讯作者	Wang, Kun(kun.wang@ia.ac.cn) ; Zhao, Xinming(xinmingzh2017@yeah.net) ; Tian, Jie(jie.tian@ia.ac.cn)
摘要	Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest human malignancies with poor patient outcomes often resulting from delayed diagnosis. Therefore, early diagnosis can lead to a better prognosis and improved outcomes. In this study, we have developed a novel conjugate complex of plectin/integrin-targeted bispecific molecular probe, termed Gd-Cy7-PTP/RGD, to be used for magnetic resonance/near-infrared imaging (MRI/NIRF) of pancreatic cancer in vivo. This bispecific molecular probe comprises four parts: Gd(III) for MRI, cyanine 7 (Cy7) for NIRF, the peptide PTP for binding to plectin-1 specifically overexpressed on the surface of PDAC cells, and the peptide RGD for targeting integrin widely expressed on pancreatic duct epithelial cells and angiogenesis. Remarkably, the combination of PTP and RGD greatly increased the targeting efficiency in vitro and in vivo compared to that of either single peptide. Moreover, such bispecific molecular probes target pancreatic neoplasms and angiogenesis simultaneously, producing a "multi-level" targeting effect confirmed by immunofluorescence testing in vitro and in vivo. Under the guidance of MRI/NIRF dual-modality imaging, NIRF-guided delineation of surgical margins during operations was successfully achieved in orthotopic pancreatic cancer. This study promotes further exploration of bispecific molecular probes for clinical application.
关键词	Bispecific peptides Early diagnosis Molecular imaging Pancreatic cancer Theranostics
DOI	10.1016/j.biomaterials.2018.08.048
关键词[WOS]	IN-VIVO ; DUCTAL ADENOCARCINOMA ; INTEGRIN ; THERAPY ; ALPHA-V-BETA-6 ; NANOPARTICLES ; METASTASIS ; STATISTICS ; CARCINOMA ; PATHWAY
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81671757] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[61671449] ; National Key Research and Development Program of China[2017YFA0205200] ; National Key Research and Development Program of China[2016YFA0201401] ; National Key Research and Development Program of China[2106YFC0103702] ; CAMS Innovation Fund for Medical Sciences[2016-12M-1-001] ; National Natural Science Foundation of China[81671757] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[61671449] ; National Key Research and Development Program of China[2017YFA0205200] ; National Key Research and Development Program of China[2016YFA0201401] ; National Key Research and Development Program of China[2106YFC0103702] ; CAMS Innovation Fund for Medical Sciences[2016-12M-1-001]
项目资助者	National Natural Science Foundation of China ; National Key Research and Development Program of China ; CAMS Innovation Fund for Medical Sciences
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:000447116600016
出版者	ELSEVIER SCI LTD
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/28141
专题	中国科学院分子影像重点实验室
通讯作者	Wang, Kun; Zhao, Xinming; Tian, Jie
作者单位	1.Chinese Acad Med Sci, Dept Imaging Diag Natl Canc Ctr, Canc Hosp, Natl Clin Res Ctr Canc, Beijing 100021, Peoples R China 2.Peking Union Med Coll, Beijing 100021, Peoples R China 3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China 4.Tsinghua Univ, Grad Sch Shenzhen, Sch Mat Sci & Engn, Beijing 100190, Peoples R China 5.Chinese Acad Sci, Key Lab Colloid Interface & Chem Thermodynam, Inst Chem, Beijing 100190, Peoples R China
第一作者单位	中国科学院分子影像重点实验室
通讯作者单位	中国科学院分子影像重点实验室
推荐引用方式 GB/T 7714	Wang, Qian,Yan, Hao,Jin, Yushen,et al. A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer[J]. BIOMATERIALS,2018,183:173-184.
APA	Wang, Qian.,Yan, Hao.,Jin, Yushen.,Wang, Zihua.,Huang, Wenhui.,...&Tian, Jie.(2018).A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer.BIOMATERIALS,183,173-184.
MLA	Wang, Qian,et al."A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer".BIOMATERIALS 183(2018):173-184.