Institutional Repository of Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
| A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer | |
Wang, Qian1,2,3; Yan, Hao3,4; Jin, Yushen3 ; Wang, Zihua5; Huang, Wenhui3; Qiu, Jia3; Kang, Feiyu4; Wang, Kun3; Zhao, Xinming1,2; Tian, Jie3
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| Source Publication | BIOMATERIALS
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| ISSN | 0142-9612 |
| 2018-11-01 | |
| Volume | 183Pages:173-184 |
| Corresponding Author | Wang, Kun(kun.wang@ia.ac.cn) ; Zhao, Xinming(xinmingzh2017@yeah.net) ; Tian, Jie(jie.tian@ia.ac.cn) |
| Abstract | Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest human malignancies with poor patient outcomes often resulting from delayed diagnosis. Therefore, early diagnosis can lead to a better prognosis and improved outcomes. In this study, we have developed a novel conjugate complex of plectin/integrin-targeted bispecific molecular probe, termed Gd-Cy7-PTP/RGD, to be used for magnetic resonance/near-infrared imaging (MRI/NIRF) of pancreatic cancer in vivo. This bispecific molecular probe comprises four parts: Gd(III) for MRI, cyanine 7 (Cy7) for NIRF, the peptide PTP for binding to plectin-1 specifically overexpressed on the surface of PDAC cells, and the peptide RGD for targeting integrin widely expressed on pancreatic duct epithelial cells and angiogenesis. Remarkably, the combination of PTP and RGD greatly increased the targeting efficiency in vitro and in vivo compared to that of either single peptide. Moreover, such bispecific molecular probes target pancreatic neoplasms and angiogenesis simultaneously, producing a "multi-level" targeting effect confirmed by immunofluorescence testing in vitro and in vivo. Under the guidance of MRI/NIRF dual-modality imaging, NIRF-guided delineation of surgical margins during operations was successfully achieved in orthotopic pancreatic cancer. This study promotes further exploration of bispecific molecular probes for clinical application. |
| Keyword | Bispecific peptides Early diagnosis Molecular imaging Pancreatic cancer Theranostics |
| DOI | 10.1016/j.biomaterials.2018.08.048 |
| WOS Keyword | IN-VIVO ; DUCTAL ADENOCARCINOMA ; INTEGRIN ; THERAPY ; ALPHA-V-BETA-6 ; NANOPARTICLES ; METASTASIS ; STATISTICS ; CARCINOMA ; PATHWAY |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[81671757] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[61671449] ; National Key Research and Development Program of China[2017YFA0205200] ; National Key Research and Development Program of China[2016YFA0201401] ; National Key Research and Development Program of China[2106YFC0103702] ; CAMS Innovation Fund for Medical Sciences[2016-12M-1-001] ; National Natural Science Foundation of China[81671757] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[61671449] ; National Key Research and Development Program of China[2017YFA0205200] ; National Key Research and Development Program of China[2016YFA0201401] ; National Key Research and Development Program of China[2106YFC0103702] ; CAMS Innovation Fund for Medical Sciences[2016-12M-1-001] |
| Funding Organization | National Natural Science Foundation of China ; National Key Research and Development Program of China ; CAMS Innovation Fund for Medical Sciences |
| WOS Research Area | Engineering ; Materials Science |
| WOS Subject | Engineering, Biomedical ; Materials Science, Biomaterials |
| WOS ID | WOS:000447116600016 |
| Publisher | ELSEVIER SCI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ia.ac.cn/handle/173211/28141 |
| Collection | 中国科学院分子影像重点实验室 |
| Corresponding Author | Wang, Kun; Zhao, Xinming; Tian, Jie |
| Affiliation | 1.Chinese Acad Med Sci, Dept Imaging Diag Natl Canc Ctr, Canc Hosp, Natl Clin Res Ctr Canc, Beijing 100021, Peoples R China 2.Peking Union Med Coll, Beijing 100021, Peoples R China 3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China 4.Tsinghua Univ, Grad Sch Shenzhen, Sch Mat Sci & Engn, Beijing 100190, Peoples R China 5.Chinese Acad Sci, Key Lab Colloid Interface & Chem Thermodynam, Inst Chem, Beijing 100190, Peoples R China |
| First Author Affilication | Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China |
| Corresponding Author Affilication | Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Qian,Yan, Hao,Jin, Yushen,et al. A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer[J]. BIOMATERIALS,2018,183:173-184. |
| APA | Wang, Qian.,Yan, Hao.,Jin, Yushen.,Wang, Zihua.,Huang, Wenhui.,...&Tian, Jie.(2018).A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer.BIOMATERIALS,183,173-184. |
| MLA | Wang, Qian,et al."A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer".BIOMATERIALS 183(2018):173-184. |
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