CASIA OpenIR  > 类脑智能研究中心  > 微观重建与智能分析
Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution
Wang, Hao1,2,3; Zhu, Qingyuan1,2; Ding, Lufeng2,3; Shen, Yan2,3; Yang, Chao-Yu2,3; Xu, Fang2,3; Shu, Chang4,5; Guo, Yujie1,2; Xiong, Zhiwei6,7; Shan, Qinghong2,3; Jia, Fan8; Su, Peng8; Yang, Qian-Ru2,3; Li, Bing2,3; Cheng, Yuxiao2,3; He, Xiaobin8; Chen, Xi4; Wu, Feng6,7,9; Zhou, Jiang-Ning2,3,9; Xu, Fuqiang8,9; Han, Hua4,9; Lau, Pak-Ming2,3; Bi, Guo-Qiang1,2,7,9
Source PublicationNATIONAL SCIENCE REVIEW
ISSN2095-5138
2019
Volume6Issue:5Pages:982-992
Abstract

The speed of high-resolution optical imaging has been a rate-limiting factor for meso-scale mapping of brain structures and functional circuits, which is of fundamental importance for neuroscience research. Here, we describe a new microscopy method of Volumetric Imaging with Synchronized on-the-fly-scan and Readout (VISoR) for high-throughput, high-quality brain mapping. Combining synchronized scanning beam illumination and oblique imaging over cleared tissue sections in smooth motion, the VISoR system effectively eliminates motion blur to obtain undistorted images. By continuously imaging moving samples without stopping, the system achieves high-speed 3D image acquisition of an entire mouse brain within 1.5 hours, at a resolution capable of visualizing synaptic spines. A pipeline is developed for sample preparation, imaging, 3D image reconstruction and quantification. Our approach is compatible with immunofluorescence methods, enabling flexible cell-type specific brain mapping and is readily scalable for large biological samples such as primate brains. Using this system, we examined behaviorally relevant whole-brain neuronal activation in 16 c-Fos-shEGFP mice under resting or forced swimming conditions. Our results indicate the involvement of multiple subcortical areas in stress response. Intriguingly, neuronal activation in these areas exhibits striking individual variability among different animals, suggesting the necessity of sufficient cohort size for such studies.

Keywordfluorescence microscopy brain mapping tissue clearing immunostaining activity trace mapping
DOI10.1093/nsr/nwz053
WOS KeywordSELECTIVE PLANE ILLUMINATION ; SEMLIKI-FOREST-VIRUS ; MOUSE ; NEURONS ; TOMOGRAPHY ; MICROSCOPY ; NETWORKS ; TISSUE ; RANGE ; MICE
Indexed BySCI
Language英语
Funding ProjectFundamental Research Funds for the Central Universities[WK6030000033] ; National Basic Research Program of China[2013CB835101] ; National Natural Science Foundation of China[91432305] ; National Natural Science Foundation of China[91632303] ; National Natural Science Foundation of China[91732304] ; Scientific Instrument Developing Project of the Chinese Academy of Sciences[YZ201668] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB02030000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB02060000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB02050000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB02050000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB02060000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB02030000] ; Scientific Instrument Developing Project of the Chinese Academy of Sciences[YZ201668] ; National Natural Science Foundation of China[91732304] ; National Natural Science Foundation of China[91632303] ; National Natural Science Foundation of China[91432305] ; National Basic Research Program of China[2013CB835101] ; Fundamental Research Funds for the Central Universities[WK6030000033]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000512966500023
PublisherOXFORD UNIV PRESS
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/28579
Collection类脑智能研究中心_微观重建与智能分析
Corresponding AuthorZhu, Qingyuan; Lau, Pak-Ming; Bi, Guo-Qiang
Affiliation1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China
3.Univ Sci & Technol China, CAS Key Lab Brain Funct & Dis, Hefei 230027, Peoples R China
4.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Univ Sci & Technol China, Sch Informat Sci & Technol, Hefei 230027, Peoples R China
7.Univ Sci & Technol China, Natl Engn Lab Brain Inspired Intelligence Technol, Hefei 230027, Peoples R China
8.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China
9.CAS Ctr Excellence Brain Sci & Intelligence Techo, Shanghai 200031, Peoples R China
Recommended Citation
GB/T 7714
Wang, Hao,Zhu, Qingyuan,Ding, Lufeng,et al. Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution[J]. NATIONAL SCIENCE REVIEW,2019,6(5):982-992.
APA Wang, Hao.,Zhu, Qingyuan.,Ding, Lufeng.,Shen, Yan.,Yang, Chao-Yu.,...&Bi, Guo-Qiang.(2019).Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution.NATIONAL SCIENCE REVIEW,6(5),982-992.
MLA Wang, Hao,et al."Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution".NATIONAL SCIENCE REVIEW 6.5(2019):982-992.
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