Dual-Modality Monitoring of Tumor Response to Cyclophosphamide Therapy in Mice with Bioluminescence Imaging and Small-Animal Positron Emission Tomography

	Dual-Modality Monitoring of Tumor Response to Cyclophosphamide Therapy in Mice with Bioluminescence Imaging and Small-Animal Positron Emission Tomography
	Ma, Xibo; Liu, Zhaofei ; Yang, Xin; Gao, Qiujuan ; Zhu, Shouping ; Qin, Chenghu ; Liu, Kai ; Zhang, Bo ; Han, Dong ; Wang, Fan 2; Tian, Jie 1
发表期刊	MOLECULAR IMAGING
	2011-07-01
卷号	10 期号:4 页码:278-283
文章类型	Article
摘要	The purpose of this study was to noninvasively monitor the therapeutic efficacy of cyclophosphamide (CTX) in a mouse model by dual-modality molecular imaging: positron emission tomography (PET) and bioluminescence imaging (BLI). Firefly luciferase (fLuc) transfected HCC-LM3-fLuc human hepatocellular carcinoma cells were injected subcutaneously into BALB/c nude mice to establish the experimental tumor model. Two groups of HCC-LM3-fLuc tumor-bearing mice (n = 7 per group) were treated with saline or CTX (100 mg/kg on days 0, 2, 5, and 7). BLI and (18)F-fluorodeoxyglucose ((18)F-FDG) PET scans were done to evaluate the treatment efficacy. CTX induced a 25.25 +/- 13.13% and 35.91 +/- 25.85% tumor growth inhibition rate on days 9 and 12 posttreatment, respectively, as determined by BLI. A good linear correlation was found between the tumor sizes measured by caliper and the BLI signals determined by optical imaging (R(2) = .9216). (18)F-FDG imaging revealed a significant uptake reduction in the tumors of the CTX-treated group compared to that in the saline control group (5.30 +/- 1.97 vs 3.00 +/- 2.11% ID/g) on day 16 after CTX treatment. Dual-modality molecular imaging using BLI and small-animal PET can play important roles in the process of chemotherapy and will provide noninvasive and reliable monitoring of the therapeutic response.
关键词	Bioluminescence
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	HEPATOCELLULAR-CARCINOMA ; METASTASIS ; INHIBITION ; GROWTH
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Radiology, Nuclear Medicine & Medical Imaging
WOS类目	Biochemical Research Methods ; Radiology, Nuclear Medicine & Medical Imaging
WOS记录号	WOS:000294562600006
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/3927
专题	中国科学院分子影像重点实验室
通讯作者	Tian, Jie
作者单位	1.Chinese Acad Sci, Med Image Proc Grp, Inst Automat, Beijing 100190, Peoples R China 2.Peking Univ, Med Isotopes Res Ctr, Beijing 100191, Peoples R China 3.Northeastern Univ, Sino Dutch Biomed & Informat Engn Sch, Shenyang, Peoples R China
推荐引用方式 GB/T 7714	Ma, Xibo,Liu, Zhaofei,Yang, Xin,et al. Dual-Modality Monitoring of Tumor Response to Cyclophosphamide Therapy in Mice with Bioluminescence Imaging and Small-Animal Positron Emission Tomography[J]. MOLECULAR IMAGING,2011,10(4):278-283.
APA	Ma, Xibo.,Liu, Zhaofei.,Yang, Xin.,Gao, Qiujuan.,Zhu, Shouping.,...&Tian, Jie.(2011).Dual-Modality Monitoring of Tumor Response to Cyclophosphamide Therapy in Mice with Bioluminescence Imaging and Small-Animal Positron Emission Tomography.MOLECULAR IMAGING,10(4),278-283.
MLA	Ma, Xibo,et al."Dual-Modality Monitoring of Tumor Response to Cyclophosphamide Therapy in Mice with Bioluminescence Imaging and Small-Animal Positron Emission Tomography".MOLECULAR IMAGING 10.4(2011):278-283.

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