CASIA OpenIR  > 中国科学院分子影像重点实验室
Noninvasive Visualization of MicroRNA-16 in the Chemoresistance of Gastric Cancer Using a Dual Reporter Gene Imaging System
Wang, Fu1,2,3; Song, Xinxing2,3; Li, Xiujuan2,3; Xin, Jing1; Wang, Shenxu2,3; Yang, Weidong4; Wang, Jing4; Wu, Kaichun5; Chen, Xiaoyuan6; Liang, Jimin1; Tian, Jie7; Cao, Feng2,3
Source PublicationPLOS ONE
2013-04-17
Volume8Issue:4Pages:-
SubtypeArticle
AbstractMicroRNAs (miRNAs) have been implicated to play a central role in the development of drug resistance in a variety of malignancies. However, many studies were conducted at the in vitro level and could not provide the in vivo information on the functions of miRNAs in the anticancer drug resistance. Here, we introduced a dual reporter gene imaging system for noninvasively monitoring the kinetic expression of miRNA-16 during chemoresistance in gastric cancer both in vitro and in vivo. Human sodium iodide symporter (hNIS) and firefly luciferase (Fluc) genes were linked to form hNIS/Fluc double fusion reporter gene and then generate human gastric cancer cell line NF-3xmir16 and its multidrug resistance cell line NF-3xmir16/VCR. Radioiodide uptake and Fluc luminescence signals in vitro correlated well with viable cell numbers. The luciferase activities and radioiodide uptake in NF-3xmir16 cells were remarkably repressed by exogenous or endogenous miRNA-16. The NF-3xmir16/VCR cells showed a significant increase of I-131 uptake and luminescence intensity compared to NF-3xmir16 cells. The radioactivity from in vivo Tc-99m-pertechnetate imaging and the intensity from bioluminescence imaging were also increased in NF-3xmir16/VCR compared with that in NF-3xmir16 tumor xenografts. Furthermore, using this reporter gene system, we found that etoposide (VP-16) and 5-fluorouracil (5-FU) activated miRNA-16 expression in vitro and in vivo, and the upregulation of miRNA-16 is p38MAPK dependent but NF-kappa B independent. This dual imaging reporter gene may be served as a novel tool for in vivo imaging of microRNAs in the chemoresistance of cancers, as well as for early detection and diagnosis in clinic.
KeywordImaging System Gastric Cancer Using
WOS HeadingsScience & Technology
WOS KeywordDRUG-RESISTANCE ; MAPK PATHWAYS ; CELLS ; CHEMOTHERAPY ; ACTIVATION
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000317907200086
Citation statistics
Cited Times:244[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/4008
Collection中国科学院分子影像重点实验室
Corresponding AuthorTian, Jie
Affiliation1.Xidian Univ, Sch Life Sci & Technol, Xian, Shaanxi, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp, Dept Cardiol, Xian 710032, Shaanxi, Peoples R China
3.Fourth Mil Med Univ, Xijing Hosp, Xian 710032, Shaanxi, Peoples R China
4.Fourth Mil Med Univ, Xijing Hosp, Dept Nucl Med, Xian 710032, Shaanxi, Peoples R China
5.Fourth Mil Med Univ, Xijing Hosp, Dept Digest Dis, Xian 710032, Shaanxi, Peoples R China
6.Natl Inst Biomed Imaging & Bioengn, Lab Mol Imaging & Nanomed, NIH, Bethesda, MD USA
7.Chinese Acad Sci, Inst Automat, Beijing, Peoples R China
Corresponding Author AffilicationInstitute of Automation, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Fu,Song, Xinxing,Li, Xiujuan,et al. Noninvasive Visualization of MicroRNA-16 in the Chemoresistance of Gastric Cancer Using a Dual Reporter Gene Imaging System[J]. PLOS ONE,2013,8(4):-.
APA Wang, Fu.,Song, Xinxing.,Li, Xiujuan.,Xin, Jing.,Wang, Shenxu.,...&Cao, Feng.(2013).Noninvasive Visualization of MicroRNA-16 in the Chemoresistance of Gastric Cancer Using a Dual Reporter Gene Imaging System.PLOS ONE,8(4),-.
MLA Wang, Fu,et al."Noninvasive Visualization of MicroRNA-16 in the Chemoresistance of Gastric Cancer Using a Dual Reporter Gene Imaging System".PLOS ONE 8.4(2013):-.
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