CASIA OpenIR  > 中国科学院分子影像重点实验室
SM5-1-Conjugated PLA nanoparticles loaded with 5-fluorouracil for targeted hepatocellular carcinoma imaging and therapy
Ma, Xibo1; Cheng, Zhen2; Jin, Yushen3; Liang, Xiaolong4; Yang, Xin1; Dai, Zhifei4; Tian, Jie1
Source PublicationBIOMATERIALS
2014-03-01
Volume35Issue:9Pages:2878-2889
SubtypeArticle
AbstractSM5-1 is a humanized mouse antibody which has a high binding specificity for a membrane protein of about 230 kDa overexpressed in hepatocellular carcinoma (HCC), melanoma and breast cancer. In this study, SM5-1-conjugated poly D, I. (lactide-coglycolide) (PLA) PLA containing Cy7 (PLA-Cy7-SM5-1) was prepared to study the targeting specificity of the bioconjugate to HCC-LM3-fLuc cell. Then, SM5-1-conjugated PLA containing 5-fluorouracil (5-FU) (PLA-5FU-SM5-1) and PLA containing 5-FU (PLA-5FU) were prepared for treatment of subcutaneous HCC-LM3-fLuc tumor mice. The results showed that PLA-5FU-SM5-1, PLA-5FU and 5-FU induced a 45.07%, 23.56% and 19.05% tumor growth inhibition rate, respectively, on day 31 post-treatment as determined by bioluminescent intensity. In addition, in order to evaluate the antitumor efficacy of PLA-5FU-SM5-1, HCC-LM3-fLuc cells were injected into the liver to establish the experimental orthotopic liver tumor models. The experiments showed that PLA-5FU-SM5-1, PLA-5FU and 5-FU induced a 53.24%, 31.00%, and 18.11% tumor growth inhibition rate, respectively, on day 31 post-treatment determined by the bioluminescent intensity of the abdomen in tumor-bearing mice. Furthermore, we have calculated the three-dimensional location of the liver cancer in mice using a multilevel adaptive finite element algorithm based on bioluminescent intensity decay calibration. The reconstruction results demonstrated that PLA-5FU-SM5-1 inhibited the tumor rapid progression, which were consistent with the results of subcutaneous tumor mice experiments and in vitro cell experiment results. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.
KeywordNanoparticles Bioluminescence Imaging (Bli) Bioluminescent Tomography (Blt) Targeted Imaging And Therapy
WOS HeadingsScience & Technology ; Technology
WOS KeywordPROTEIN-PROTEIN INTERACTIONS ; POSITRON-EMISSION-TOMOGRAPHY ; GROWTH-FACTOR RECEPTOR ; BIOLUMINESCENCE TOMOGRAPHY ; IN-VIVO ; BREAST-CANCER ; MONOCLONAL-ANTIBODY ; 1ST-LINE TREATMENT ; COLORECTAL-CANCER ; TUMOR-METASTASIS
Indexed BySCI
Language英语
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000332188900031
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/4033
Collection中国科学院分子影像重点实验室
Corresponding AuthorTian, Jie
Affiliation1.Chinese Acad Sci, Inst Automat, Key Lab Mol Imaging, Beijing 100190, Peoples R China
2.Stanford Univ, Dept Radiol, MIPS, BioX Program, Stanford, CA 94305 USA
3.Harbin Inst Technol, Sch Life Sci & Technol, Nanomed & Biosensor Lab, Harbin 150080, Peoples R China
4.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China
First Author AffilicationInstitute of Automation, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Automation, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Ma, Xibo,Cheng, Zhen,Jin, Yushen,et al. SM5-1-Conjugated PLA nanoparticles loaded with 5-fluorouracil for targeted hepatocellular carcinoma imaging and therapy[J]. BIOMATERIALS,2014,35(9):2878-2889.
APA Ma, Xibo.,Cheng, Zhen.,Jin, Yushen.,Liang, Xiaolong.,Yang, Xin.,...&Tian, Jie.(2014).SM5-1-Conjugated PLA nanoparticles loaded with 5-fluorouracil for targeted hepatocellular carcinoma imaging and therapy.BIOMATERIALS,35(9),2878-2889.
MLA Ma, Xibo,et al."SM5-1-Conjugated PLA nanoparticles loaded with 5-fluorouracil for targeted hepatocellular carcinoma imaging and therapy".BIOMATERIALS 35.9(2014):2878-2889.
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