ApoG2 as the most potent gossypol derivatives inhibits cell growth and induces apoptosis on gastric cancer cells

	ApoG2 as the most potent gossypol derivatives inhibits cell growth and induces apoptosis on gastric cancer cells
	Xin, J.1; Zhan, Y. H.1; Xia, L. M.3,4; Zhu, H. W.3,4,5; Nie, Y. Z.3,4; Liang, J. M.1; Tian, J.1,2; Tian, Jie
发表期刊	BIOMEDICINE & PHARMACOTHERAPY
	2013-02-01
卷号	67 期号:1 页码:88-95
文章类型	Article
摘要	Gastric cancer is one of the most common types of malignancies and proteins from the Bcl-2 family are highly expressed in human gastric cancer. Apogossypolone (ApoG2), the most potent gossypol derivative, has been defined as a novel small-molecule inhibitor of anti-apoptotic Bcl-2 family proteins. However, whether or not it can inhibit the growth and proliferation of gastric cancer cell lines has not been demonstrated to date. Here, we assessed the effects of anti-growth of ApoG2 on gastric cancer cell lines in vitro and explored the possible molecular mechanisms of ApoG2. Using the MTT assay and flow cytometry, we found that ApoG2 has the significant anti-growth effect on MKN28, MKN45 and AGS cell lines in a time-and dose-dependent manner. Compared to (-)-gossypol, MTT assay and flow cytometry results showed that anti-growth effect of ApoG2 is inferior, but the colony formation ability of ApoG2 is superior. Furthermore, western blot results revealed that ApoG2 inhibits the growth and proliferation of gastric cancer cells by down-regulating of Bcl-2 protein expression, up-regulating of Bax and activating of Caspase-3. Taken together, albeit the ApoG2 inferior to (-)-gossypol in many ways on gastric cancer in vitro, our results suggest that ApoG2 could effectively inhibit the growth and proliferation of gastric cancer cell lines through the mitochondrial pathway of apoptosis. (C) 2012 Elsevier Masson SAS. All rights reserved.
关键词	Apog2 Small-molecule Inhibitor Gastric Cancer
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	BCL-2 FAMILY PROTEINS ; PROSTATE-CANCER ; BREAST-CANCER ; CYCLE ARREST ; U937 CELLS ; IN-VITRO ; APOGOSSYPOLONE ; THERAPY ; CARCINOMA ; PATHWAYS
收录类别	SCI
语种	英语
WOS研究方向	Research & Experimental Medicine ; Pharmacology & Pharmacy
WOS类目	Medicine, Research & Experimental ; Pharmacology & Pharmacy
WOS记录号	WOS:000317096300014
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/4039
专题	中国科学院分子影像重点实验室
通讯作者	Tian, Jie
作者单位	1.Xidian Univ, Sch Life Sci & Technol, Xian 710071, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China 3.Fourth Mil Med Univ, Xijing Hosp, Dept Gastroenterol, Xian 710032, Shaanxi, Peoples R China 4.Fourth Mil Med Univ, Xijing Hosp, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R China 5.Guangzhou Mil Command, Guangzhou Gen Hosp, Dept Gastroenterol, Guangzhou 510010, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Xin, J.,Zhan, Y. H.,Xia, L. M.,et al. ApoG2 as the most potent gossypol derivatives inhibits cell growth and induces apoptosis on gastric cancer cells[J]. BIOMEDICINE & PHARMACOTHERAPY,2013,67(1):88-95.
APA	Xin, J..,Zhan, Y. H..,Xia, L. M..,Zhu, H. W..,Nie, Y. Z..,...&Tian, Jie.(2013).ApoG2 as the most potent gossypol derivatives inhibits cell growth and induces apoptosis on gastric cancer cells.BIOMEDICINE & PHARMACOTHERAPY,67(1),88-95.
MLA	Xin, J.,et al."ApoG2 as the most potent gossypol derivatives inhibits cell growth and induces apoptosis on gastric cancer cells".BIOMEDICINE & PHARMACOTHERAPY 67.1(2013):88-95.

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