CASIA OpenIR  > 中国科学院分子影像重点实验室
Helical optical projection tomography
Arranz, Alicia1; Dong, Di2; Zhu, Shouping3; Rudin, Markus1; Tsatsanis, Christos4; Tian, Jie2,3; Ripoll, Jorge5,6,7; Jorge Ripoll
Source PublicationOPTICS EXPRESS
2013-11-04
Volume21Issue:22Pages:25912-25925
SubtypeArticle
AbstractA new technique termed Helical Optical Projection Tomography (hOPT) has been developed with the aim to overcome some of the limitations of current 3D optical imaging techniques. hOPT is based on Optical Projection Tomography (OPT) with the major difference that there is a translation of the sample in the vertical direction during the image acquisition process, requiring a new approach to image reconstruction. Contrary to OPT, hOPT makes possible to obtain 3D-optical images of intact long samples without imposing limits on the sample length. This has been tested using hOPT to image long murine tissue samples such as spinal cords and large intestines. Moreover, 3D-reconstructed images of the colon of DSS-treated mice, a model for Inflammatory Bowel Disease, allowed the identification of the structural alterations. Finally, the geometry of the hOPT device facilitates the addition of a Selective Plane Illumination Microscopy (SPIM) arm, providing the possibility of delivering high resolution images of selected areas together with complete volumetric information. (C) 2013 Optical Society of America
KeywordFluorescence Microscopy Three-dimensional Microscopy Image Reconstruction Techniques Light Propagation In Tissues
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1364/OE.21.025912
WOS KeywordPLANE ILLUMINATION MICROSCOPY ; LIGHT-SHEET MICROSCOPY ; GENE-EXPRESSION ; RESOLUTION ; VISUALIZATION ; SPECIMENS ; BRAIN ; DEEP
Indexed BySCI
Language英语
WOS Research AreaOptics
WOS SubjectOptics
WOS IDWOS:000327007800053
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/4104
Collection中国科学院分子影像重点实验室
Corresponding AuthorJorge Ripoll
Affiliation1.Swiss Fed Inst Technol, Inst Biomed Engn, CH-8093 Zurich, Switzerland
2.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China
3.Xidian Univ, Sch Life Sci & Technol, Xian 710071, Shaanxi, Peoples R China
4.Univ Crete, Sch Med, Lab Clin Chem, Iraklion 71003, Greece
5.Fdn Res & Technol Hellas FORTH, Inst Elect Struct & Laser, Iraklion 71110, Greece
6.Univ Carlos III Madrid, Dept Bioengn & Aerosp Engn, Madrid 28911, Spain
7.Hosp Gen Gregorio Maranon, Inst Invest Sanitaria, Expt Med & Surg Unit, Madrid 28007, Spain
Recommended Citation
GB/T 7714
Arranz, Alicia,Dong, Di,Zhu, Shouping,et al. Helical optical projection tomography[J]. OPTICS EXPRESS,2013,21(22):25912-25925.
APA Arranz, Alicia.,Dong, Di.,Zhu, Shouping.,Rudin, Markus.,Tsatsanis, Christos.,...&Jorge Ripoll.(2013).Helical optical projection tomography.OPTICS EXPRESS,21(22),25912-25925.
MLA Arranz, Alicia,et al."Helical optical projection tomography".OPTICS EXPRESS 21.22(2013):25912-25925.
Files in This Item: Download All
File Name/Size DocType Version Access License
oe-21-22-25912.pdf(3590KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Arranz, Alicia]'s Articles
[Dong, Di]'s Articles
[Zhu, Shouping]'s Articles
Baidu academic
Similar articles in Baidu academic
[Arranz, Alicia]'s Articles
[Dong, Di]'s Articles
[Zhu, Shouping]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Arranz, Alicia]'s Articles
[Dong, Di]'s Articles
[Zhu, Shouping]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: oe-21-22-25912.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.