Application of Horseradish Peroxidase Labeling Along With Post-Staining Improves Data Quality in Micro-Brain Connectomics

doi:10.16476/j.pibb.2020.0011

	Application of Horseradish Peroxidase Labeling Along With Post-Staining Improves Data Quality in Micro-Brain Connectomics
	Wang Sheng-Xiong 1,2; Chen Zhen-Qiang 3; Han Hua4 ; Guo Ai-Ke 1,2,5,6,7
发表期刊	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN	1000-3282
	2020-10-01
卷号	47 期号:10 页码:1090-1096
通讯作者	Han Hua() ; Guo Ai-Ke(akguo@ion.ac.cn)
摘要	For micro-brain connectomic research, volume electron microscopy is a main method in which it plays an importatnt role for the appliaction of automated tape-collecting ultramicrotome (ATUM) system and arrayimaging with scanning electron microscopy together. ATUM system enables collecting a large amount of special sections to be imaged with SEM to form ahuge dataset. With regard tothe nature of non-vacuum sectoningand sample reserving. we modified and improved the sample preparatio process includingresinrecipe, HRP labelling and post-staing method i orderto reduce folding rate of sample sections and make it possible for identifyig certain types of neurons in high-contrast imaging dataset, thus obtainig dataset of high quality for connectomic research.
关键词	brain connectomics volume electron microscopy sample preparation
DOI	10.16476/j.pibb.2020.0011
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of Chinese Academy of Science[XDB32010100]
项目资助者	Strategic Priority Research Program of Chinese Academy of Science
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000583458700007
出版者	CHINESE ACAD SCIENCES, INST BIOPHYSICS
七大方向——子方向分类	脑网络分析
引用统计
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/41749
专题	脑图谱与类脑智能实验室_微观重建与智能分析
通讯作者	Han Hua; Guo Ai-Ke
作者单位	1.Chinese Acad Sci, State Key Lab Brain & Cognit Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Luwan Branch, Shanghai 200020, Peoples R China 4.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China 5.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, State Key Lab Neurosci, Inst Neurosci, Shanghai 200031, Peoples R China 6.Shanghai Ctr Brain Sci & Inspired Intelligence Te, Shanghai 201210, Peoples R China 7.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China
通讯作者单位	中国科学院自动化研究所
推荐引用方式 GB/T 7714	Wang Sheng-Xiong,Chen Zhen-Qiang,Han Hua,et al. Application of Horseradish Peroxidase Labeling Along With Post-Staining Improves Data Quality in Micro-Brain Connectomics[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2020,47(10):1090-1096.
APA	Wang Sheng-Xiong,Chen Zhen-Qiang,Han Hua,&Guo Ai-Ke.(2020).Application of Horseradish Peroxidase Labeling Along With Post-Staining Improves Data Quality in Micro-Brain Connectomics.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,47(10),1090-1096.
MLA	Wang Sheng-Xiong,et al."Application of Horseradish Peroxidase Labeling Along With Post-Staining Improves Data Quality in Micro-Brain Connectomics".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 47.10(2020):1090-1096.