The first visualization of chemotherapy-induced tumor apoptosis via magnetic particle imaging in a mouse model

doi:10.1088/1361-6560/abad7c

	The first visualization of chemotherapy-induced tumor apoptosis via magnetic particle imaging in a mouse model
	Liang, Xin 1,2; Wang, Kun3 ; Du, Jiangfeng 2; Tian, Jie 3; Zhang, Hui1,2
发表期刊	PHYSICS IN MEDICINE AND BIOLOGY
ISSN	0031-9155
	2020-10-07
卷号	65 期号:19 页码:11
通讯作者	Tian, Jie(jie.tian@ia.ac.cn) ; Zhang, Hui(zhanghui_mr@163.com)
摘要	Imaging technologies that allow non-radiative visualization and quantification of apoptosis have a great potential for assessing therapy response, early diagnosis, and disease monitoring. Magnetic particle imaging (MPI), the direct imaging of magnetic nanoparticles as positive contrast agent and sole signal source, enables high image contrast (no tissue background signal), potential high sensitivity, and quantifiable signal intensity. These properties confer a great potential for application to tumor apoptosis monitoring. In this study, a simple and robust method was used to conjugate Alexa Fluor 647-AnnexinV (AF647-Anx), which can avidly bind to apoptotic cells, to superparamagnetic iron oxide (SPIO) nanoparticles, termed AF647-Anx-SPIO, which serves as an MPI-detectable tracer. Based on this apoptosis-specific tracer, MPI can accurately and unambiguously detect and quantify apoptotic tumor cells. AF647-Anx-SPIO showed relatively high affinity for apoptotic cells, and differences in binding between treated (apoptotic rate 67.21% +/- 1.36%) and untreated (apoptotic rate 10.12 +/- 0.11%) cells could be detected by MPIin vitro(P< 0.05). Moreover, the imaging signal was almost proportional to the number of apoptotic cells determined using an MPI scanner (R-2= 0.99). There was a greater accumulation of AF647-Anx-SPIO in tumors of drug-treated animals than in tumors of untreated animals (P< 0.05), and the difference could be detected by MPIex vivo, while forin vivoimaging, no MPI imaging signal was detected in either group. Overall, this preliminary study demonstrates that MPI could be a potential imaging modality for tumor apoptosis imaging.
关键词	magnetic particle imaging apoptosis nanoparticle preclinical mice tumor
DOI	10.1088/1361-6560/abad7c
关键词[WOS]	IN-VIVO ; ANNEXIN-V ; HIGH-PERFORMANCE ; NANOPARTICLES ; CANCER ; PHOSPHATIDYLSERINE ; BIODISTRIBUTION ; EXPRESSION ; CLEARANCE ; PATHWAYS
收录类别	SCI
语种	英语
资助项目	Ministry of Science and Technology of China[2017YFA0205200] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[61671449] ; Chinese Academy of Sciences[GJJSTD20170004] ; Chinese Academy of Sciences[KFJ-STS-ZDTP-059] ; Chinese Academy of Sciences[YJKYYQ20180048] ; Chinese Academy of Sciences[XDB32030200] ; Chinese Academy of Sciences[QYZDJ-SSW-JSC005]
项目资助者	Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences
WOS研究方向	Engineering ; Radiology, Nuclear Medicine & Medical Imaging
WOS类目	Engineering, Biomedical ; Radiology, Nuclear Medicine & Medical Imaging
WOS记录号	WOS:000575512700001
出版者	IOP PUBLISHING LTD
七大方向——子方向分类	医学影像处理与分析
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/42029
专题	中国科学院分子影像重点实验室
通讯作者	Tian, Jie; Zhang, Hui
作者单位	1.Shanxi Med Univ, Clin Med Coll 1, Dept Radiol, Taiyuan, Shanxi, Peoples R China 2.Shanxi Med Univ, Coll Med Imaging, Taiyuan, Shanxi, Peoples R China 3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing, Peoples R China
通讯作者单位	中国科学院自动化研究所
推荐引用方式 GB/T 7714	Liang, Xin,Wang, Kun,Du, Jiangfeng,et al. The first visualization of chemotherapy-induced tumor apoptosis via magnetic particle imaging in a mouse model[J]. PHYSICS IN MEDICINE AND BIOLOGY,2020,65(19):11.
APA	Liang, Xin,Wang, Kun,Du, Jiangfeng,Tian, Jie,&Zhang, Hui.(2020).The first visualization of chemotherapy-induced tumor apoptosis via magnetic particle imaging in a mouse model.PHYSICS IN MEDICINE AND BIOLOGY,65(19),11.
MLA	Liang, Xin,et al."The first visualization of chemotherapy-induced tumor apoptosis via magnetic particle imaging in a mouse model".PHYSICS IN MEDICINE AND BIOLOGY 65.19(2020):11.