CASIA OpenIR  > 中国科学院分子影像重点实验室
The effect of endothelial progenitor cell transplantation on neointimal hyperplasia and reendothelialisation after balloon catheter injury in rat carotid arteries
Wang,Wei1,2,3; Zhang,Yingqian2; Hui,Hui3,4; Tong,Wei1,2,3; Wei,Zechen3,4; Li,Zhongxuan2; Zhang,Suhui1,2,3; Yang,Xin3,4; Tian,Jie3,4,5; Chen,Yundai2
Source PublicationStem Cell Research & Therapy
2021-02-03
Volume12Issue:1Pages:12
Corresponding AuthorTian,Jie(jie.tian@ia.ac.cn) ; Chen,Yundai(cyundai@vip.163.com)
AbstractAbstractBackgroundReendothelialisation is the natural pathway that inhibits neointimal hyperplasia and in-stent restenosis. Circulating endothelial progenitor cells (EPCs) derived from bone marrow (BM) might contribute to endothelial repair. However, the temporal and spatial distributions of reendothelialisation and neointimal hyperplasia after EPC transplantation in injured arteries are currently unclear.MethodsA carotid balloon injury (BI) model was established in Sprague-Dawley rats, and PKH26-labelled BM-derived EPCs were transplanted after BI. The carotid arteries were harvested on the first, fourth, seventh, and 14th day post-injury and analysed via light-sheet fluorescence microscopy and pathological staining (n?=?3). EPC and human umbilical vein endothelial cell culture supernatants were collected, and blood samples were collected before and after transplantation. The paracrine effects of VEGF, IGF-1, and TGF-β1 in cell culture supernatants and serum were analysed by enzyme-linked immunosorbent assay (n?=?4).ResultsTransplanted EPCs labelled with PKH26 were attached to the injured luminal surface the first day after BI. In the sham operation group, the transplanted EPCs did not adhere to the luminal surface. From the fourth day after BI, the mean fluorescence intensity of PKH26 decreased significantly. However, reendothelialisation and inhibition of neointimal hyperplasia were significantly promoted by transplanted EPCs. The degree of reendothelialisation of the EPC7d and EPC14d groups was higher than that of the BI7d and BI14d groups, and the difference in neointimal hyperplasia was observed between the EPC14d and BI14d groups. The number of endothelial cells on the luminal surface of the EPC14d group was higher than that of the BI14d group. The number of infiltrated macrophages in the injured artery decreased in the EPC transplanted groups.ConclusionsTransplanted EPCs had chemotactic enrichment and attached to the injured arterial luminal surface. Although decreasing significantly after the fourth day at the site of injury after transplantation, transplanted EPCs could still promote reendothelialisation and inhibit neointimal hyperplasia. The underlying mechanism is through paracrine cytokines and not differentiation into mature endothelial cells.
KeywordEndothelial progenitor cells Reendothelialisation Neointimal hyperplasia Angioplasty Light-sheet fluorescence microscopy
DOI10.1186/s13287-021-02135-w
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2017YFA0700401] ; National Key Research and Development Program of China[2016YFC0103803] ; National Key Research and Development Program of China[2017YFA0205200] ; National Natural Science Foundation of China[81800221] ; National Natural Science Foundation of China[81671731] ; National Natural Science Foundation of China[81827808] ; National Natural Science Foundation of China[81671851] ; National Natural Science Foundation of China[81870178] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[81971680] ; National Natural Science Foundation of China[81970443] ; Capital Clinical Feature Research Project[Z171100001017158] ; CAS Strategic Priority Research Program[XDB32030200] ; CAS Scientific Instrument RD Program[YJKYYQ20170075] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2018167]
Funding OrganizationNational Key Research and Development Program of China ; National Natural Science Foundation of China ; Capital Clinical Feature Research Project ; CAS Strategic Priority Research Program ; CAS Scientific Instrument RD Program ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
WOS Research AreaCell Biology ; Research & Experimental Medicine
WOS SubjectCell & Tissue Engineering ; Cell Biology ; Medicine, Research & Experimental
WOS IDBMC:10.1186/s13287-021-02135-w
PublisherBioMed Central
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/42240
Collection中国科学院分子影像重点实验室
Corresponding AuthorTian,Jie; Chen,Yundai
Affiliation1.Chinese PLA General Hospital; Medical School of Chinese PLA
2.Chinese PLA General Hospital; Department of Cardiology, the Sixth Medical Centre
3.Chinese Academy of Sciences; CAS Key Laboratory of Molecular Imaging, Institute of Automation
4.University of Chinese Academy of Sciences
5.Beihang University; Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, School of Medicine
Recommended Citation
GB/T 7714
Wang,Wei,Zhang,Yingqian,Hui,Hui,et al. The effect of endothelial progenitor cell transplantation on neointimal hyperplasia and reendothelialisation after balloon catheter injury in rat carotid arteries[J]. Stem Cell Research & Therapy,2021,12(1):12.
APA Wang,Wei.,Zhang,Yingqian.,Hui,Hui.,Tong,Wei.,Wei,Zechen.,...&Chen,Yundai.(2021).The effect of endothelial progenitor cell transplantation on neointimal hyperplasia and reendothelialisation after balloon catheter injury in rat carotid arteries.Stem Cell Research & Therapy,12(1),12.
MLA Wang,Wei,et al."The effect of endothelial progenitor cell transplantation on neointimal hyperplasia and reendothelialisation after balloon catheter injury in rat carotid arteries".Stem Cell Research & Therapy 12.1(2021):12.
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