Regulation of hepatic differentiation of human embryonic stem cells by calcium silicate extracts for liver injury repairing

doi:10.1016/j.apmt.2020.100730

	Regulation of hepatic differentiation of human embryonic stem cells by calcium silicate extracts for liver injury repairing
	Zheng, Lu 1,2,3; Li, Dongyuan 1,2,3; Zhang, Fan 1,2,3; Xing, Min 4; Wang, Xiaoya 4; Jia, Xiaohua5 ; Shu, Xinyu 1,2,3; Li, Peiwen 1,2,3; Li, Shouqin 1,2,3; Chang, Jiang 4; Long, Mian 1,2,3
发表期刊	APPLIED MATERIALS TODAY
ISSN	2352-9407
	2020-09-01
卷号	20 页码:15
通讯作者	Chang, Jiang(jchang@mail.sic.ac.cn) ; Long, Mian(mlong@imech.ac.cn)
摘要	Directing embryonic stem cell (ESC)-derived hepatocytes is critical in understanding hepatic differentiation and applying cell-based treatment to severe liver diseases. While growth factor-based strategies are widely used, using chemical cues could present an alternative to optimize the strategies for stem cell differentiation. Here, for the first time, an inorganic calcium silicate (CS, CaSiO3)-based approach, together with a modified four-stage differentiating protocol, was proposed to quantify the effects of CS extracts on inducing hepatic differentiation of human ESCs (H9 cells). The roles of CS-activated H9 cells in liver injury repair were tested by cell tracking and immunohistochemical staining. Results indicated that high concentrations of CS extracts initially enhance definitive endodermal (DE) lineage, followed by gradual DE differentiation at low CS concentrations. The order of CS addition is also crucial, since the presence at stemness stage and the absence at DE stage could optimize hepatic differentiation capacity of H9 cells, resulting in optimized cells that differentiate into functional hepatocyte-like cells. The addition of CS extracts at precursor hepatocyte stage enhances their maturity, which favors the turnover of liver injury in CCl4-treated mice. These results provide an insight into applying bioactive inorganic biomaterials to foster hepatic differentiation of human ESCs for cell therapy. (c) 2020 Elsevier Ltd. All rights reserved.
关键词	Human embryonic stem cells Hepatic differentiation Hepatocyte-like cells Calcium silicate extracts Liver injury repair
DOI	10.1016/j.apmt.2020.100730
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[91642203] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[31870931] ; Frontier Science Key Project of Chinese Science Academy[QYZDJ-SSW-JSC018]
项目资助者	National Natural Science Foundation of China ; Frontier Science Key Project of Chinese Science Academy
WOS研究方向	Materials Science
WOS类目	Materials Science, Multidisciplinary
WOS记录号	WOS:000598353800004
出版者	ELSEVIER
七大方向——子方向分类	其他
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/42805
专题	中国科学院分子影像重点实验室
通讯作者	Chang, Jiang; Long, Mian
作者单位	1.Chinese Acad Sci, Beijing Key Lab Engineered Construct & Mechanobio, Natl Micrograv Lab, Ctr Biomech & Bioengn,Inst Mech, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Key Lab Micrograv, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Ceram, Biomat & Tissue Engn Res Ctr, Shanghai 200050, Peoples R China 5.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Lu,Li, Dongyuan,Zhang, Fan,et al. Regulation of hepatic differentiation of human embryonic stem cells by calcium silicate extracts for liver injury repairing[J]. APPLIED MATERIALS TODAY,2020,20:15.
APA	Zheng, Lu.,Li, Dongyuan.,Zhang, Fan.,Xing, Min.,Wang, Xiaoya.,...&Long, Mian.(2020).Regulation of hepatic differentiation of human embryonic stem cells by calcium silicate extracts for liver injury repairing.APPLIED MATERIALS TODAY,20,15.
MLA	Zheng, Lu,et al."Regulation of hepatic differentiation of human embryonic stem cells by calcium silicate extracts for liver injury repairing".APPLIED MATERIALS TODAY 20(2020):15.