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Targeted Luminescent Probes for Precise Upconversion/NIR II Luminescence Diagnosis of Lung Adenocarcinoma
Lv, Ruichan1; Wang, Yanxing1; Lin, Bi1; Peng, Xiangrong1; Liu, Jun1; Lu, Wei-dong2; Tian, Jie3
发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
2021-03-23
卷号93期号:11页码:4984-4992
通讯作者Lv, Ruichan(rclv@xidian.edu.cn) ; Tian, Jie(jie.tian@ia.ac.cn)
摘要In this research, the antibody of the searched hub genes has been proposed to combine with a rare-earth composite for an upconversion luminescence (UCL) and downconversion (DCL) NIR-II imaging strategy for the diagnosis of lung adenocarcinoma (LUAD). Weighted gene co-expression network analysis is used to search the most relevant hub genes, and the required top genes that contribute to tumorigenesis (negative: CLEC3B, MFAP4, PECAM1, and FHL1; positive: CCNB2, CDCA5, HMMR, and TOP2A) are identified and validated by survival analysis and transcriptional and translational results. Meanwhile, fluorescence imaging probes (NaYF4:Yb,Er,Eu@ NaYF4:Nd, denoted as NYF:Eu NPs) with multimodal optical imaging properties of downconversion and upconversion luminescence in the visible region and luminescence in the near infrared II region are designed with various uniform sizes and enhanced penetration and sensitivity. Finally, when the NYF:Eu NP probe is combined with antibodies of these chosen positive hub genes (such as, TOP2A and CCNB2), the in vitro and in vivo animal experiments (flow cytometry, cell counting kit-8 assay using A549 cells, and in vivo immunohistochemistry IHC microscopy images of LUAD from patient cases) indicate that the designed nanoprobes can be excellently used as a targeted optical probe for future accurate diagnosis and surgery navigation of LUAD in contrast with other cancer cells and normal cells. This strategy of antibodies combined with optical probes provides a dual-modal luminescence imaging method for precise medicine.
DOI10.1021/acs.analchem.1c00374
收录类别SCI
语种英语
资助项目Natural Science Foundation of China[NSFC 81801744] ; National Key R&D Program of China[2017YFA0205202] ; National Key R&D Program of China[2017YFC1309100]
项目资助者Natural Science Foundation of China ; National Key R&D Program of China
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000634564500033
出版者AMER CHEMICAL SOC
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ia.ac.cn/handle/173211/44194
专题中国科学院分子影像重点实验室
通讯作者Lv, Ruichan; Tian, Jie
作者单位1.Xidian Univ, Sch Life Sci & Technol, Minist Educ, Engn Res Ctr Mol & Neuro Imaging, Xian 710071, Shanxi, Peoples R China
2.Xi An Jiao Tong Univ, Med Coll, Tumor Hosp Shaanxi Prov, Dept Thorac Surg, Xian 710061, Shaanxi, Peoples R China
3.Chinese Acad Sci, Key Lab Mol Imaging, Inst Automat, Beijing 100190, Peoples R China
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GB/T 7714
Lv, Ruichan,Wang, Yanxing,Lin, Bi,et al. Targeted Luminescent Probes for Precise Upconversion/NIR II Luminescence Diagnosis of Lung Adenocarcinoma[J]. ANALYTICAL CHEMISTRY,2021,93(11):4984-4992.
APA Lv, Ruichan.,Wang, Yanxing.,Lin, Bi.,Peng, Xiangrong.,Liu, Jun.,...&Tian, Jie.(2021).Targeted Luminescent Probes for Precise Upconversion/NIR II Luminescence Diagnosis of Lung Adenocarcinoma.ANALYTICAL CHEMISTRY,93(11),4984-4992.
MLA Lv, Ruichan,et al."Targeted Luminescent Probes for Precise Upconversion/NIR II Luminescence Diagnosis of Lung Adenocarcinoma".ANALYTICAL CHEMISTRY 93.11(2021):4984-4992.
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