Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography

doi:10.1039/d1bm00174d

	Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography
	Luo, Chunhua 1,2; Lu, Dongyuan 1,2,3; Zheng, Lu 1,2,3; Zhang, Fan 1,2,3; Zhang, Xiao 1,2,3; Lu, Shouqin 1,2,3; Zhang, Chen 1,2; Jia, Xiaohua4 ; Shu, Xinyu 1,2,3; Li, Peiwen 1,2; Li, Zhan 1,2; Long, Mian 1,2,3
发表期刊	BIOMATERIALS SCIENCE
ISSN	2047-4830
	2021-04-20
页码	15
通讯作者	Long, Mian(mlong@imech.ac.cn)
摘要	Mechanical or physical cues are associated with the growth and differentiation of embryonic stem cells (ESCs). While the substrate stiffness or topography independently affects the differentiation of ESCs, their cooperative regulation on lineage-specific differentiation remains largely unknown. Here, four topographical configurations on stiff or soft polyacrylamide hydrogel were combined to direct hepatic differentiation of human H1 cells via a four-stage protocol, and the coupled impacts of stiffness and topography were quantified at distinct stages. Data indicated that the substrate stiffness is dominant in stemness maintenance on stiff gel and hepatic differentiation on soft gel while substrate topography assists the differentiation of hepatocyte-like cells in positive correlation with the circularity of H1 clones initially formed on the substrate. The differentiated cells exhibited liver-specific functions such as maintaining the capacities of CYP450 metabolism, glycogen synthesis, ICG engulfment, and repairing liver injury in CCl4-treated mice. These results implied that the coupling of substrate stiffness and topography, combined with the biochemical signals, is favorable to improve the efficiency and functionality of hepatic differentiation of human ESCs.
DOI	10.1039/d1bm00174d
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[91642203] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[31870931] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31470907] ; National Natural Science Foundation of China[81703012] ; Chinese Science Academy[QYZDJ-SSW-JSC018] ; Chinese Science Academy[GJJSTU20190005] ; Chinese Science Academy[XDB22040101]
项目资助者	National Natural Science Foundation of China ; Chinese Science Academy
WOS研究方向	Materials Science
WOS类目	Materials Science, Biomaterials
WOS记录号	WOS:000641302300001
出版者	ROYAL SOC CHEMISTRY
七大方向——子方向分类	其他
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/44267
专题	中国科学院分子影像重点实验室
通讯作者	Long, Mian
作者单位	1.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Luo, Chunhua,Lu, Dongyuan,Zheng, Lu,et al. Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography[J]. BIOMATERIALS SCIENCE,2021:15.
APA	Luo, Chunhua.,Lu, Dongyuan.,Zheng, Lu.,Zhang, Fan.,Zhang, Xiao.,...&Long, Mian.(2021).Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography.BIOMATERIALS SCIENCE,15.
MLA	Luo, Chunhua,et al."Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography".BIOMATERIALS SCIENCE (2021):15.