In vivo fluorescence molecular imaging of the vascular endothelial growth factor in rats with early diabetic retinopathy

doi:10.1364/BOE.439446

	In vivo fluorescence molecular imaging of the vascular endothelial growth factor in rats with early diabetic retinopathy
	Zhang, Lu 1,2,3; Ding, Yunhe 1,2,3; Chen, Xinjian 4,5; Xiang, Dehui 4,5; Shi, Fei 4,5; Chen, Yanyun 6; Yan, Shenshen 6; Zhang, Xinyuan 6; Tian, Jie 3; Siva Prasad, Sobha 7; Du, Yang3 ; Yang, Zhi 1,2; Tian, Bei 6
发表期刊	BIOMEDICAL OPTICS EXPRESS
ISSN	2156-7085
	2021-11-01
卷号	12 期号:11 页码:7185-7198
通讯作者	Du, Yang(yang.du@ia.ac.cn)
摘要	Anti-vascular endothelial growth factor (anti-VEGF) therapy is effective for reducing the severity level of diabetic retinopathy (DR). However, it is difficult to determine the in vivo spatial and temporal expression of VEGF in the DR retina at an early stage. Here, we report a quantitatively fluorescence molecular imaging and image analysis method by creating a VEGF targeted fluorescence imaging probe, which can potentially detect and predict anti-VEGF treatment response. Moreover, the ex vivo multiscale fluorescence imaging demonstrated the spatial correlation between VEGF relative expression and vascular abnormalities in two and three dimensions. It revealed that VEGF was mainly abnormally expressed at the bifurcation of the microvessels, which advances the knowledge of the DR progression by molecular fluorescence imaging. Our study has the potential to achieve early detection of DR disease, provide more insight into understanding anti-VEGF treatment, and may help stratify patients based on the molecular imaging of retinal VEGF.
DOI	10.1364/BOE.439446
关键词[WOS]	MAJOR RISK-FACTORS ; GLOBAL PREVALENCE ; VEGF ; NEUTRALIZATION ; RANIBIZUMAB ; BEVACIZUMAB ; AFLIBERCEPT ; PROGRESSION
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[61771326] ; National Natural Science Foundation of China[61827809] ; Science and Technology Project of Beijing Municipal Commission of Education[KM202010025026]
项目资助者	National Natural Science Foundation of China ; Science and Technology Project of Beijing Municipal Commission of Education
WOS研究方向	Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS类目	Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS记录号	WOS:000717641100003
出版者	OPTICAL SOC AMER
七大方向——子方向分类	医学影像处理与分析
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/46516
专题	中国科学院分子影像重点实验室
通讯作者	Du, Yang
作者单位	1.Capital Med Univ, Sch Biomed Engn, Beijing 100069, Peoples R China 2.Capital Med Univ, Beijing Key Lab Fundamental Res Biomech Clin Appl, Beijing 100069, Peoples R China 3.Inst Automat, Chinese Acad Sci, State Key Lab Management & Control Complex Syst, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China 4.Soochow Univ, Sch Elect & Informat Engn, Suzhou 215006, Jiangsu, Peoples R China 5.Soochow Univ, State Key Lab Radiat Med & Protect, Suzhou 215006, Jiangsu, Peoples R China 6.Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing 100730, Peoples R China 7.Moorfields Eye Hosp, NIHR Moorfields Biomed Res Ctr, London EC1V 2PD, England
第一作者单位	中国科学院自动化研究所
通讯作者单位	中国科学院自动化研究所
推荐引用方式 GB/T 7714	Zhang, Lu,Ding, Yunhe,Chen, Xinjian,et al. In vivo fluorescence molecular imaging of the vascular endothelial growth factor in rats with early diabetic retinopathy[J]. BIOMEDICAL OPTICS EXPRESS,2021,12(11):7185-7198.
APA	Zhang, Lu.,Ding, Yunhe.,Chen, Xinjian.,Xiang, Dehui.,Shi, Fei.,...&Tian, Bei.(2021).In vivo fluorescence molecular imaging of the vascular endothelial growth factor in rats with early diabetic retinopathy.BIOMEDICAL OPTICS EXPRESS,12(11),7185-7198.
MLA	Zhang, Lu,et al."In vivo fluorescence molecular imaging of the vascular endothelial growth factor in rats with early diabetic retinopathy".BIOMEDICAL OPTICS EXPRESS 12.11(2021):7185-7198.