Novel fluorescent GLUT1 inhibitor for precision detection and fluorescence image-guided surgery in oral squamous cell carcinoma

doi:10.1002/ijc.34049

	Novel fluorescent GLUT1 inhibitor for precision detection and fluorescence image-guided surgery in oral squamous cell carcinoma
	Tian, Yu 1,2,3; Tang, Chu 4; Shi, Guangyuan 5; Wang, Guorong 6; Du, Yang3,7,8 ; Tian, Jie 3,4,7,8,9; Zhang, Haizhong 2
发表期刊	INTERNATIONAL JOURNAL OF CANCER
ISSN	0020-7136
	2022-08-01
卷号	151 期号:3 页码:450-462
通讯作者	Du, Yang(yang.du@ia.ac.cn) ; Tian, Jie(jie.tian@ia.ac.cn) ; Zhang, Haizhong(zhanghaizhong@301hospital.com.cn)
摘要	Early detection and complete resection of oral squamous cell carcinoma (OSCC) are crucial to improving patient survival and prognosis. However, specifically targeted imaging probes for OSCC detection are limited. Our study aimed to synthesize a novel near-infrared fluorescence (NIRF) probe for precision detection and fluorescence image-guided surgery in OSCC. Bioinformatics data indicated that glucose transporter 1 (GLUT1) is highly expressed in patients with OSCC. We demonstrated high and specific GLUT1 expression upon immunohistochemical staining of samples from 20 patients with OSCC. The specific expression of GLUT1 was further validated in both human OSCC cell lines and OSCC tumor xenografts. Based on these findings, the GLUT1 inhibitor WZB117 was utilized to synthesize a novel NIRF imaging probe, WZB117-IR820. The fluorescence molecular imaging data revealed that WZB117-IR820 could specifically bind to the tumor areas in an orthotopic OSCC mouse model after intravenous injection and could be further applied for precision fluorescence image-guided surgery with no residual tumor in the orthotopic CAL27-fLUC mouse tumor model. For further clinical translational application in patients with OSCC, precise delineation of OSCC tumor areas was achieved after topical application of the WZB117-IR820 imaging probe and was validated by histopathological and immunohistochemical analyses. In conclusion, we synthesized a novel fluorescent imaging probe, WZB117-IR820, which has potential clinical applications for early detection and fluorescence image-guided surgery in OSCC with no observable toxicity.
关键词	fluorescence molecular imaging glucose transporter type 1 image-guided surgery mouth neoplasms WZB117
DOI	10.1002/ijc.34049
关键词[WOS]	SURGICAL NAVIGATION ; CANCER ; HEAD ; MARGINS ; TRANSPORT ; VALIDATION ; SURVIVAL ; BINDING ; FAMILY ; SAFETY
收录类别	SCI
语种	英语
资助项目	Beijing Natural Science Foundation[7212207] ; Ministry of Science and Technology of China[2017YFA0205200] ; Ministry of Science and Technology of China[2017YFA0700401] ; China National Key Research and Development Plan Project[2017YFB1304300] ; National Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[81470083] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[81871514] ; Subsidiary of PLA Major Project[AWS17J004]
项目资助者	Beijing Natural Science Foundation ; Ministry of Science and Technology of China ; China National Key Research and Development Plan Project ; National Natural Science Foundation of China ; Subsidiary of PLA Major Project
WOS研究方向	Oncology
WOS类目	Oncology
WOS记录号	WOS:000795148100001
出版者	WILEY
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/49408
专题	中国科学院分子影像重点实验室
通讯作者	Du, Yang; Tian, Jie; Zhang, Haizhong
作者单位	1.Med Sch Chinese PLA, Beijing, Peoples R China 2.Chinese Peoples Liberat Army Gen Hosp, Med Ctr 1, Dept Stomatol, 28 Fuxing Rd, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, State Key Lab Management & Control Complex Syst, Beijing, Peoples R China 4.Xidian Univ, Engn Res Ctr Mol & Neuro Imaging, Sch Life Sci & Technol, Minist Educ, Xian, Peoples R China 5.Univ Sci & Technol China, Sch Informat Sci & Technol, Hefei, Peoples R China 6.Peking Union Med Coll Hosp, Peking Union Med Coll & Chinese Acad Med Sci, Dept Radiol, Beijing, Peoples R China 7.Beijing Key Lab Mol Imaging, Beijing, Peoples R China 8.Univ Chinese Acad Sci, Beijing, Peoples R China 9.Beihang Univ, Beijing Adv Innovat Ctr Big Data Based Precis Med, Sch Med, Beijing, Peoples R China
第一作者单位	中国科学院自动化研究所
通讯作者单位	中国科学院自动化研究所
推荐引用方式 GB/T 7714	Tian, Yu,Tang, Chu,Shi, Guangyuan,et al. Novel fluorescent GLUT1 inhibitor for precision detection and fluorescence image-guided surgery in oral squamous cell carcinoma[J]. INTERNATIONAL JOURNAL OF CANCER,2022,151(3):450-462.
APA	Tian, Yu.,Tang, Chu.,Shi, Guangyuan.,Wang, Guorong.,Du, Yang.,...&Zhang, Haizhong.(2022).Novel fluorescent GLUT1 inhibitor for precision detection and fluorescence image-guided surgery in oral squamous cell carcinoma.INTERNATIONAL JOURNAL OF CANCER,151(3),450-462.
MLA	Tian, Yu,et al."Novel fluorescent GLUT1 inhibitor for precision detection and fluorescence image-guided surgery in oral squamous cell carcinoma".INTERNATIONAL JOURNAL OF CANCER 151.3(2022):450-462.