Institutional Repository of Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
| Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation | |
Wang, Guorong1,2; Li, Wenzhe3; Shi, Guangyuan2,4; Tian, Yu2,5; Kong, Lingyan1; Ding, Ning1; Lei, Jing1; Jin, Zhengyu1; Tian, Jie2,6,7 ; Du, Yang2,6
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| Source Publication | EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
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| ISSN | 1619-7070 |
| 2022-05-20 | |
| Pages | 12 |
| Corresponding Author | Jin, Zhengyu(jin_zhengyu@163.com) ; Tian, Jie(jie.tian@ia.ac.cn) ; Du, Yang(yang.du@ia.ac.cn) |
| Abstract | Purpose A sensitive and specific imaging method to detect metastatic cancer cells in lymph nodes to detect the early-stage breast cancer is still a challenge. The purpose of this study was to investigate a novel breast cancer-targeting and tumour microenvironment ATP-responsive superparamagnetic iron oxide nanoparticles (SPIOs) imaging probe (abbreviated as SPIOs@A-T) that was developed to detect lymph node metastasis through fluorescence molecular imaging (FMI) and magnetic particle imaging (MPI). Methods The conjugation of the targeted peptide CREKA and SPIOs was via linker sulfo-SMCC, while the dsDNA-Cy5.5 was modified on SPIOs through the conjugation between maleimide group in sulfo-SMCC and sulfydryl group in dsDNA-Cy5.5. SPIOs@A-T was characterised for its imaging properties, targeting ability and toxicity in vitro. Mice with metastatic lymph node (MLN) of breast cancer were established to evaluate the FMI and MPI imaging strategy in vivo. Healthy mice with normal lymph node (NLN) were used as control group. Histological examination and biosafety evaluation were performed for further assessment. Results After injection with SPIOs@A-T, the obvious high fluorescent intensity and MPI signal were observed in MLN group than those in NLN group. FMI can specifically light up MLN using an ATP-responsive fluorescence design. On the other hand, MPI could complement the limitation of imaging depth from FMI and could detect MLN more sensitively. Besides, the biosafety evaluation results showed SPIOs@A-T had no detectable biological toxicity. Conclusion SPIOs@A-T imaging probe in combination with FMI and MPI can provide a promising novel method for the precise detection of MLN in vivo. |
| Keyword | Lymph node metastasis Breast cancer Magnetic particle imaging (MPI) Fluorescence molecular imaging (FMI) Superparamagnetic iron oxide nanoparticles (SPIOs) |
| DOI | 10.1007/s00259-022-05834-5 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Beijing Natural Science Foundation[7212207] ; National Natural Science Foundation of China[81871422] ; National Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[81871514] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[81470083] ; National Natural Science Foundation of China[81527805] ; Ministry of Science and Technology of China[2017YFC1309100] ; Ministry of Science and Technology of China[2017YFC1309104] ; Ministry of Science and Technology of China[2017YFA0205200] ; Ministry of Science and Technology of China[2017YFA0700401] |
| Funding Organization | Beijing Natural Science Foundation ; National Natural Science Foundation of China ; Ministry of Science and Technology of China |
| WOS Research Area | Radiology, Nuclear Medicine & Medical Imaging |
| WOS Subject | Radiology, Nuclear Medicine & Medical Imaging |
| WOS ID | WOS:000798203700001 |
| Publisher | SPRINGER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ia.ac.cn/handle/173211/49423 |
| Collection | 中国科学院分子影像重点实验室 |
| Corresponding Author | Jin, Zhengyu; Tian, Jie; Du, Yang |
| Affiliation | 1.Peking Union Med Coll & Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Radiol, Beijing 100730, Peoples R China 2.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing Key Lab Mol Imaging,State Key Lab Managem, Beijing 100190, Peoples R China 3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China 4.Univ Sci & Technol China, Hefei 230026, Peoples R China 5.Med Sch Chinese PLA, Beijing 100853, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100080, Peoples R China 7.Beihang Univ, Beijing Adv Innovat Ctr Big Data Based Precis Med, Sch Med, Beijing 100083, Peoples R China |
| First Author Affilication | Institute of Automation, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Automation, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Wang, Guorong,Li, Wenzhe,Shi, Guangyuan,et al. Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation[J]. EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,2022:12. |
| APA | Wang, Guorong.,Li, Wenzhe.,Shi, Guangyuan.,Tian, Yu.,Kong, Lingyan.,...&Du, Yang.(2022).Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation.EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,12. |
| MLA | Wang, Guorong,et al."Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation".EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING (2022):12. |
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