Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation

doi:10.1007/s00259-022-05834-5

	Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation
	Wang, Guorong 1,2; Li, Wenzhe 3; Shi, Guangyuan 2,4; Tian, Yu 2,5; Kong, Lingyan 1; Ding, Ning 1; Lei, Jing 1; Jin, Zhengyu 1; Tian, Jie 2,6,7; Du, Yang2,6
发表期刊	EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
ISSN	1619-7070
	2022-05-20
页码	12
通讯作者	Jin, Zhengyu(jin_zhengyu@163.com) ; Tian, Jie(jie.tian@ia.ac.cn) ; Du, Yang(yang.du@ia.ac.cn)
摘要	Purpose A sensitive and specific imaging method to detect metastatic cancer cells in lymph nodes to detect the early-stage breast cancer is still a challenge. The purpose of this study was to investigate a novel breast cancer-targeting and tumour microenvironment ATP-responsive superparamagnetic iron oxide nanoparticles (SPIOs) imaging probe (abbreviated as SPIOs@A-T) that was developed to detect lymph node metastasis through fluorescence molecular imaging (FMI) and magnetic particle imaging (MPI). Methods The conjugation of the targeted peptide CREKA and SPIOs was via linker sulfo-SMCC, while the dsDNA-Cy5.5 was modified on SPIOs through the conjugation between maleimide group in sulfo-SMCC and sulfydryl group in dsDNA-Cy5.5. SPIOs@A-T was characterised for its imaging properties, targeting ability and toxicity in vitro. Mice with metastatic lymph node (MLN) of breast cancer were established to evaluate the FMI and MPI imaging strategy in vivo. Healthy mice with normal lymph node (NLN) were used as control group. Histological examination and biosafety evaluation were performed for further assessment. Results After injection with SPIOs@A-T, the obvious high fluorescent intensity and MPI signal were observed in MLN group than those in NLN group. FMI can specifically light up MLN using an ATP-responsive fluorescence design. On the other hand, MPI could complement the limitation of imaging depth from FMI and could detect MLN more sensitively. Besides, the biosafety evaluation results showed SPIOs@A-T had no detectable biological toxicity. Conclusion SPIOs@A-T imaging probe in combination with FMI and MPI can provide a promising novel method for the precise detection of MLN in vivo.
关键词	Lymph node metastasis Breast cancer Magnetic particle imaging (MPI) Fluorescence molecular imaging (FMI) Superparamagnetic iron oxide nanoparticles (SPIOs)
DOI	10.1007/s00259-022-05834-5
收录类别	SCI
语种	英语
资助项目	Beijing Natural Science Foundation[7212207] ; National Natural Science Foundation of China[81871422] ; National Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[81871514] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[81470083] ; National Natural Science Foundation of China[81527805] ; Ministry of Science and Technology of China[2017YFC1309100] ; Ministry of Science and Technology of China[2017YFC1309104] ; Ministry of Science and Technology of China[2017YFA0205200] ; Ministry of Science and Technology of China[2017YFA0700401]
项目资助者	Beijing Natural Science Foundation ; National Natural Science Foundation of China ; Ministry of Science and Technology of China
WOS研究方向	Radiology, Nuclear Medicine & Medical Imaging
WOS类目	Radiology, Nuclear Medicine & Medical Imaging
WOS记录号	WOS:000798203700001
出版者	SPRINGER
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/49423
专题	中国科学院分子影像重点实验室
通讯作者	Jin, Zhengyu; Tian, Jie; Du, Yang
作者单位	1.Peking Union Med Coll & Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Radiol, Beijing 100730, Peoples R China 2.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing Key Lab Mol Imaging,State Key Lab Managem, Beijing 100190, Peoples R China 3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China 4.Univ Sci & Technol China, Hefei 230026, Peoples R China 5.Med Sch Chinese PLA, Beijing 100853, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100080, Peoples R China 7.Beihang Univ, Beijing Adv Innovat Ctr Big Data Based Precis Med, Sch Med, Beijing 100083, Peoples R China
第一作者单位	中国科学院自动化研究所
通讯作者单位	中国科学院自动化研究所
推荐引用方式 GB/T 7714	Wang, Guorong,Li, Wenzhe,Shi, Guangyuan,et al. Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation[J]. EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,2022:12.
APA	Wang, Guorong.,Li, Wenzhe.,Shi, Guangyuan.,Tian, Yu.,Kong, Lingyan.,...&Du, Yang.(2022).Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation.EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,12.
MLA	Wang, Guorong,et al."Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation".EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING (2022):12.