CASIA OpenIR  > 中国科学院分子影像重点实验室
Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation
Wang, Guorong1,2; Li, Wenzhe3; Shi, Guangyuan2,4; Tian, Yu2,5; Kong, Lingyan1; Ding, Ning1; Lei, Jing1; Jin, Zhengyu1; Tian, Jie2,6,7; Du, Yang2,6
Source PublicationEUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
ISSN1619-7070
2022-05-20
Pages12
Corresponding AuthorJin, Zhengyu(jin_zhengyu@163.com) ; Tian, Jie(jie.tian@ia.ac.cn) ; Du, Yang(yang.du@ia.ac.cn)
AbstractPurpose A sensitive and specific imaging method to detect metastatic cancer cells in lymph nodes to detect the early-stage breast cancer is still a challenge. The purpose of this study was to investigate a novel breast cancer-targeting and tumour microenvironment ATP-responsive superparamagnetic iron oxide nanoparticles (SPIOs) imaging probe (abbreviated as SPIOs@A-T) that was developed to detect lymph node metastasis through fluorescence molecular imaging (FMI) and magnetic particle imaging (MPI). Methods The conjugation of the targeted peptide CREKA and SPIOs was via linker sulfo-SMCC, while the dsDNA-Cy5.5 was modified on SPIOs through the conjugation between maleimide group in sulfo-SMCC and sulfydryl group in dsDNA-Cy5.5. SPIOs@A-T was characterised for its imaging properties, targeting ability and toxicity in vitro. Mice with metastatic lymph node (MLN) of breast cancer were established to evaluate the FMI and MPI imaging strategy in vivo. Healthy mice with normal lymph node (NLN) were used as control group. Histological examination and biosafety evaluation were performed for further assessment. Results After injection with SPIOs@A-T, the obvious high fluorescent intensity and MPI signal were observed in MLN group than those in NLN group. FMI can specifically light up MLN using an ATP-responsive fluorescence design. On the other hand, MPI could complement the limitation of imaging depth from FMI and could detect MLN more sensitively. Besides, the biosafety evaluation results showed SPIOs@A-T had no detectable biological toxicity. Conclusion SPIOs@A-T imaging probe in combination with FMI and MPI can provide a promising novel method for the precise detection of MLN in vivo.
KeywordLymph node metastasis Breast cancer Magnetic particle imaging (MPI) Fluorescence molecular imaging (FMI) Superparamagnetic iron oxide nanoparticles (SPIOs)
DOI10.1007/s00259-022-05834-5
Indexed BySCI
Language英语
Funding ProjectBeijing Natural Science Foundation[7212207] ; National Natural Science Foundation of China[81871422] ; National Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[81871514] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[81470083] ; National Natural Science Foundation of China[81527805] ; Ministry of Science and Technology of China[2017YFC1309100] ; Ministry of Science and Technology of China[2017YFC1309104] ; Ministry of Science and Technology of China[2017YFA0205200] ; Ministry of Science and Technology of China[2017YFA0700401]
Funding OrganizationBeijing Natural Science Foundation ; National Natural Science Foundation of China ; Ministry of Science and Technology of China
WOS Research AreaRadiology, Nuclear Medicine & Medical Imaging
WOS SubjectRadiology, Nuclear Medicine & Medical Imaging
WOS IDWOS:000798203700001
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/49423
Collection中国科学院分子影像重点实验室
Corresponding AuthorJin, Zhengyu; Tian, Jie; Du, Yang
Affiliation1.Peking Union Med Coll & Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Radiol, Beijing 100730, Peoples R China
2.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing Key Lab Mol Imaging,State Key Lab Managem, Beijing 100190, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
4.Univ Sci & Technol China, Hefei 230026, Peoples R China
5.Med Sch Chinese PLA, Beijing 100853, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100080, Peoples R China
7.Beihang Univ, Beijing Adv Innovat Ctr Big Data Based Precis Med, Sch Med, Beijing 100083, Peoples R China
First Author AffilicationInstitute of Automation, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Automation, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Guorong,Li, Wenzhe,Shi, Guangyuan,et al. Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation[J]. EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,2022:12.
APA Wang, Guorong.,Li, Wenzhe.,Shi, Guangyuan.,Tian, Yu.,Kong, Lingyan.,...&Du, Yang.(2022).Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation.EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,12.
MLA Wang, Guorong,et al."Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation".EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING (2022):12.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Guorong]'s Articles
[Li, Wenzhe]'s Articles
[Shi, Guangyuan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Guorong]'s Articles
[Li, Wenzhe]'s Articles
[Shi, Guangyuan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Guorong]'s Articles
[Li, Wenzhe]'s Articles
[Shi, Guangyuan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.