Peptide-Conjugated Aggregation-Induced Emission Fluorogenic Probe for Glypican-3 Protein Detection and Hepatocellular Carcinoma Cells Imaging

doi:10.3390/chemosensors10050195

	Peptide-Conjugated Aggregation-Induced Emission Fluorogenic Probe for Glypican-3 Protein Detection and Hepatocellular Carcinoma Cells Imaging
	Zhang, Song 1; Jing, Jiangbo 1; Meng, Lingchen 1; Xu, Bin 1; Ma, Xibo2,3,4 ; Tian, Wenjing 1
Source Publication	CHEMOSENSORS
	2022-05-01
Volume	10 Issue:5 Pages:10
Corresponding Author	Ma, Xibo(xibo.ma@ia.ac.cn) ; Tian, Wenjing(wjtian@jlu.edu.cn)
Abstract	Hepatocellular carcinoma (HCC) is a malignant tumor with high morbidity and mortality on a global scale, and the development of accurate detection and imaging methods for HCC cells is urgently needed. Herein, by connecting peptide L5, which can specifically bind to the overexpressed Glypican-3 (GPC-3) protein of HCC cells with aggregation-induced emission (AIE) moiety ammonium cation-functionalized 9,10-distyrylanthracene (NDSA) via the "click" reaction, we synthesized a fluorescent probe NDSA-L5. In an aqueous solution, the probe shows weak emission, whereas, in the presence of the GPC-3 protein, bright fluorescence can be obtained since NDSA-L5 binds to the GPC-3 protein, leading to the restricted intramolecular movement of AIE-active NDSA-L5. The imaging and flow cytometry experiments demonstrate that the NDSA-L5 probe can rapidly accumulate in the subcutaneous HCC cells and liver tumor tissue and shows a potential application in early detection and surgical navigation for HCC cancer.
Keyword	hepatocellular carcinoma (HCC) Glypican-3 aggregation-induced emission (AIE) cells imaging
DOI	10.3390/chemosensors10050195
WOS Keyword	PHOTODYNAMIC THERAPY ; LIVER-CANCER ; LABEL-FREE
Indexed By	SCI
Language	英语
Funding Project	Natural Science Foundation of China[21835001] ; Natural Science Foundation of China[52073116] ; Natural Science Foundation of China[51773080] ; Natural Science Foundation of China[82090051] ; Natural Science Foundation of China[81871442] ; JLU Science and Technology Innovative Research Team[2021TD-03] ; National Key Research programs of China[2016YFA0100900] ; National Key Research programs of China[2016YFA0100902] ; Youth Innovation Promotion Association CAS[Y201930]
Funding Organization	Natural Science Foundation of China ; JLU Science and Technology Innovative Research Team ; National Key Research programs of China ; Youth Innovation Promotion Association CAS
WOS Research Area	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS Subject	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS ID	WOS:000801364700001
Publisher	MDPI
Citation statistics	Cited Times:1[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.ia.ac.cn/handle/173211/49540
Collection	模式识别国家重点实验室_生物识别与安全技术
Corresponding Author	Ma, Xibo; Tian, Wenjing
Affiliation	1.Jilin Univ, Coll Chem, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China 2.Chinese Acad Sci, Inst Automat, CBSR, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Automat, NLPR, Beijing 100049, Peoples R China 4.Univ Chinese Acad Sci, Sch Artificial Intelligence, Beijing 100049, Peoples R China
Corresponding Author Affilication	Institute of Automation, Chinese Academy of Sciences; Chinese Acad Sci, Inst Automat, Natl Lab Pattern Recognit, Beijing 100190, Peoples R China
Recommended Citation GB/T 7714	Zhang, Song,Jing, Jiangbo,Meng, Lingchen,et al. Peptide-Conjugated Aggregation-Induced Emission Fluorogenic Probe for Glypican-3 Protein Detection and Hepatocellular Carcinoma Cells Imaging[J]. CHEMOSENSORS,2022,10(5):10.
APA	Zhang, Song,Jing, Jiangbo,Meng, Lingchen,Xu, Bin,Ma, Xibo,&Tian, Wenjing.(2022).Peptide-Conjugated Aggregation-Induced Emission Fluorogenic Probe for Glypican-3 Protein Detection and Hepatocellular Carcinoma Cells Imaging.CHEMOSENSORS,10(5),10.
MLA	Zhang, Song,et al."Peptide-Conjugated Aggregation-Induced Emission Fluorogenic Probe for Glypican-3 Protein Detection and Hepatocellular Carcinoma Cells Imaging".CHEMOSENSORS 10.5(2022):10.

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