CASIA OpenIR  > 中国科学院分子影像重点实验室
Optical magnetic multimodality imaging of plectin-1-targeted imaging agent for the precise detection of orthotopic pancreatic ductal adenocarcinoma in mice
Zhang, Wenjia1,2,4; Liang, Xiaolong3; Zhu, Liang1,2; Zhang, Xinyu1,2; Jin, Zhengyu1,2; De, Yang4,5; Tian, Jie4,6; Xue, Huadan1,2
Source PublicationEBIOMEDICINE
ISSN2352-3964
2022-06-01
Volume80Pages:16
Corresponding AuthorJin, Zhengyu(jinzy@pumch.cn) ; De, Yang(yang.du@ia.ac.cn) ; Tian, Jie(jie.tian@ia.ac.cn) ; Xue, Huadan(bjdanna95@hotmail.com)
AbstractBackground Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy worldwide, and the precise detection is challenging currently. Magnetic particle imaging (MPI) is suitable for imaging deep and internal PDAC tumours because of its high sensitivity and unlimited imaging depth. The purpose of this study was to utilize the MPI, in combination with fluorescence molecular imaging (FMI) and magnetic resonance imaging (MRI), to advance the in vivo precise detection of PDAC xenografts. Methods The PDAC targeted plectin-1 peptide and IRDye800CW were conjugated to the superparamagnetic iron oxide nanoparticles (PTP-Fe3O4-IRDye800CW) for the PDAC-targeting triple-modality imaging. Subcutaneous and orthotopic PDAC mouse models were established. FMI, MPI, and MRI were performed for dynamic and quantitative observation of PDAC tumours. Histological staining analyses were used for ex vivo validation. Findings PTP-Fe3O4-IRDye800CW nanoparticles possessed great triple-modality imaging performance and specific targeting to plectin-1 expressed on PDAC cells. For in vivo multi-modality imaging of orthotopic PDAC models, the PTP-Fe3O4-IRDye800CW nanoparticles demonstrated higher specificity, even distribution, and longer retention effects in tumours for over 7 d compared with Con-Fe3O4-IRDye800CW nanoparticles. (MPI, 2d post-injection: PTP-Fe3O4-IRDye800CW: 85.72% +/- 1.53% vs. Con-Fe3O4-IRDye800CW: 74.41% +/- 1.91%, **P < 0.01 (Student's t test)). Ex vivo histological and Prussian blue stainings were performed to validate the distribution of probes. Interpretation These data demonstrate the feasibility of utilizing MPI for in vivo PDAC imaging and complement with FMI/MRI for a precise and comprehensive in vivo characterization of PDAC. This may benefit PDAC patients for precise diagnosis and guidance of therapy. Copyright (c) 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
KeywordPancreatic ductal adenocarcinoma (PDAC) Magnetic particle imaging (MPI) Superparamagnetic iron oxide nanoparticles (SPIONs) Fluorescence molecular imaging (FMI) Magnetic resonance imaging (MRI)
DOI10.1016/j.ebiom.2022.104040
WOS KeywordIRON-OXIDE NANOPARTICLES ; CANCER ; FERUMOXYTOL ; MRI
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[82071896] ; National Natural Science Foundation of China[81871422] ; National Natural Science Foundation of China[81871514] ; National Natural Science Foundation of China[81227901] ; Ministry of Science and Technology of China[2017YFA0205200] ; Ministry of Science and Technology of China[2017YFA0700401] ; Beijing Natural Science Foundation[7212207] ; Elite Program of Dong Cheng District of Beijing[2020-dchrcpyzz-28] ; Peking University Third Hospital[BYSYZD2019018] ; Peking University Third Hospital[jyzc2018-02]
Funding OrganizationNational Natural Science Foundation of China ; Ministry of Science and Technology of China ; Beijing Natural Science Foundation ; Elite Program of Dong Cheng District of Beijing ; Peking University Third Hospital
WOS Research AreaGeneral & Internal Medicine ; Research & Experimental Medicine
WOS SubjectMedicine, General & Internal ; Medicine, Research & Experimental
WOS IDWOS:000829908400014
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/49753
Collection中国科学院分子影像重点实验室
Corresponding AuthorJin, Zhengyu; De, Yang; Tian, Jie; Xue, Huadan
Affiliation1.Peking Union Med Coll, Dept Radiol, State Key Lab Complex Severe & Rare Dis, Peking Union Med Coll Hosp, 1 Shuaifuyuan, Beijing 100730, Peoples R China
2.Chinese Acad Med Sci, 1 Shuaifuyuan, Beijing 100730, Peoples R China
3.Peking Univ Third Hosp, Dept Ultrasound, Beijing 100191, Peoples R China
4.Chinese Acad Sci, State Key Lab Management & Control Complex Syst, Beijing Key Lab Mol Imaging, CAS Key Lab Mol Imaging,Inst Automat, 95 Zhongguancun East Rd, Beijing 100190, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Beihang Univ, Beijing Adv Innovat Ctr Big Data Based Precis Med, Sch Med, Beijing 100191, Peoples R China
First Author AffilicationInstitute of Automation, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Automation, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Wenjia,Liang, Xiaolong,Zhu, Liang,et al. Optical magnetic multimodality imaging of plectin-1-targeted imaging agent for the precise detection of orthotopic pancreatic ductal adenocarcinoma in mice[J]. EBIOMEDICINE,2022,80:16.
APA Zhang, Wenjia.,Liang, Xiaolong.,Zhu, Liang.,Zhang, Xinyu.,Jin, Zhengyu.,...&Xue, Huadan.(2022).Optical magnetic multimodality imaging of plectin-1-targeted imaging agent for the precise detection of orthotopic pancreatic ductal adenocarcinoma in mice.EBIOMEDICINE,80,16.
MLA Zhang, Wenjia,et al."Optical magnetic multimodality imaging of plectin-1-targeted imaging agent for the precise detection of orthotopic pancreatic ductal adenocarcinoma in mice".EBIOMEDICINE 80(2022):16.
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