Fear memory-associated synaptic and mitochondrial changes revealed by deep learning-based processing of electron microscopy data

doi:10.1016/j.celrep.2022.111151

	Fear memory-associated synaptic and mitochondrial changes revealed by deep learning-based processing of electron microscopy data
	Liu, Jing1,2 ; Qi, Junqian 3,4,5; Chen, Xi1 ; Li, Zhenchen 1,2; Hong, Bei 1; Ma, Hongtu1 ; Li, Guoqing1 ; Shen, Lijun1 ; Liu, Danqian 4; Kong, Yu 4; Zhai, Hao 1,2; Xie, Qiwei 6; Han, Hua1,2,4 ; Yang, Yang 3
发表期刊	CELL REPORTS
ISSN	2211-1247
	2022-08-02
卷号	40 期号:5 页码:28
通讯作者	Xie, Qiwei(qiwei.xie@bjut.edu.cn) ; Han, Hua(hua.han@ia.ac.cn) ; Yang, Yang(yangyang2@shanghaitech.edu.cn)
摘要	Serial section electron microscopy (ssEM) can provide comprehensive 3D ultrastructural information of the brain with exceptional computational cost. Targeted reconstruction of subcellular structures from ssEM da-tasets is less computationally demanding but still highly informative. We thus developed a region-CNN -based deep learning method to identify, segment, and reconstruct synapses and mitochondria to explore the structural plasticity of synapses and mitochondria in the auditory cortex of mice subjected to fear con-ditioning. Upon reconstructing over 135,000 mitochondria and 160,000 synapses, we find that fear condition-ing significantly increases the number of mitochondria but decreases their size and promotes formation of multi-contact synapses, comprising a single axonal bouton and multiple postsynaptic sites from different dendrites. Modeling indicates that such multi-contact configuration increases the information storage ca-pacity of new synapses by over 50%. With high accuracy and speed in reconstruction, our method yields structural and functional insight into cellular plasticity associated with fear learning.
DOI	10.1016/j.celrep.2022.111151
关键词[WOS]	STRUCTURAL DETERMINANTS ; MULTIPLE SYNAPSES ; CEREBRAL CORTEX ; MOSSY FIBER ; LAYER 4 ; PLASTICITY ; EXPERIENCE ; MORPHOGENESIS ; SEGMENTATION ; TRANSMISSION
收录类别	SCI
语种	英语
资助项目	National Science and Technology Innovation 2030 Major Program[2021ZD0204503] ; National Science and Technology Innovation 2030 Major Program[2021ZD0 204500] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB32030208] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA16021104] ; National Natural Science Foundation of China[32171461] ; National Natural Science Foundation of China[31970960] ; National Natural Science Foundation of China[61673381] ; Program of Beijing Municipal Science & Technology Commission[Z201100008420004] ; ShanghaiTech University
项目资助者	National Science and Technology Innovation 2030 Major Program ; Strategic Priority Research Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; Program of Beijing Municipal Science & Technology Commission ; ShanghaiTech University
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000843351100001
出版者	CELL PRESS
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/49871
专题	脑图谱与类脑智能实验室_微观重建与智能分析
通讯作者	Xie, Qiwei; Han, Hua; Yang, Yang
作者单位	1.Chinese Acad Sci, Inst Automat, Res Ctr Brain inspired Intelligence, Lab Pattern Recognit, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sch Artificial Intelligence, Sch Future Technol, Beijing 101408, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 4.Chinese Acad Sci, Inst Neurosci, Ctr Excellence Brain Sci & Intelligence Technol, State Key Lab Neurosci,Key Lab Primate Neurobiol, Shanghai 200031, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Beijing Univ Technol, Res Base Beijing Modern Mfg Dev, Beijing 100124, Peoples R China
第一作者单位	中国科学院自动化研究所
通讯作者单位	中国科学院自动化研究所
推荐引用方式 GB/T 7714	Liu, Jing,Qi, Junqian,Chen, Xi,et al. Fear memory-associated synaptic and mitochondrial changes revealed by deep learning-based processing of electron microscopy data[J]. CELL REPORTS,2022,40(5):28.
APA	Liu, Jing.,Qi, Junqian.,Chen, Xi.,Li, Zhenchen.,Hong, Bei.,...&Yang, Yang.(2022).Fear memory-associated synaptic and mitochondrial changes revealed by deep learning-based processing of electron microscopy data.CELL REPORTS,40(5),28.
MLA	Liu, Jing,et al."Fear memory-associated synaptic and mitochondrial changes revealed by deep learning-based processing of electron microscopy data".CELL REPORTS 40.5(2022):28.