CASIA OpenIR  > 中国科学院分子影像重点实验室
The Detection of Divalent Iron and Reactive Oxygen Species During Ferroptosis with the Use of a Dual-Reaction Turn-On Fluorescent Probe
Wang, Yueqi1,2; Li, Changjian3,4; Zhuo, Jiaming1; Hui, Hui1,2; Zhou, Bing3; Tian, Jie1,2,3,4,5
Source PublicationMOLECULAR IMAGING AND BIOLOGY
ISSN1536-1632
2022-10-04
Pages12
Corresponding AuthorHui, Hui(hui.hui@ia.ac.cn) ; Zhou, Bing(bingzh@buaa.edu.cn) ; Tian, Jie(tian@ieee.org)
AbstractPurpose Ferroptosis, a programmed cell death modality, is an iron-dependent, non-apoptosis pathway that is characterized by the upregulation of divalent iron and reactive oxygen species (ROS) levels. However, the sensitive and rapid detection to track changes in ferroptosis is challenging, partially due to the lack of methods for monitoring the Fe(II) accumulation and ROS generation. Procedures Herein, we reported a dual-reaction fluorescent probe DR-1 with turn-on response, which realized the simultaneous visualizing of Fe(II) and ROS with a single probe. The structure of fluorescence quenching group and turn-on fluorophore constitute a double switch for DR-1, which increases its specificity and stability. Results During ferroptotic cell death, the upregulation of ROS levels led to the cleavage of quenching group of DR-1, and the aggregation of Fe(II) resulting in fluorescence recovery. Conclusions Overall, this study provides a new dual-reaction probe that shows the great potential to explore the mechanism of ferroptosis in vitro and in vivo by fluorescence imaging.
KeywordDual-reaction Fe(II) Ferroptosis Fluorescent probes ROS Turn-on response
DOI10.1007/s11307-022-01774-6
WOS KeywordLABILE IRON ; CELL-DEATH ; LIPID-PEROXIDATION ; CANCER-CELLS ; GPX4 ; DYSFUNCTION ; EXPRESSION ; FE(II) ; STATE
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2017YFA0700401] ; National Key Research and Development Program of China[2016YFC0103803] ; National Key Research and Development Program of China[2017YFA0205200] ; National Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[81930053] ; National Natural Science Foundation of China[81827808] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[81971198] ; National Natural Science Foundation of China[81671851] ; China Postdoctoral Science Foundation[2020M680301] ; China Postdoctoral Science Foundation[2019TQ0018] ; Chinese Academy of Sciences Youth Innovation Promotion Association[2018167] ; Chinese Academy of Sciences Key Technology Talent Program ; Project of High-Level Talents Team Introduction in Zhuhai City[Zhuhai HLHPTP201703] ; Fundamental Research Funds for the Central Universities[JKF-YG-22-B005]
Funding OrganizationNational Key Research and Development Program of China ; National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; Chinese Academy of Sciences Youth Innovation Promotion Association ; Chinese Academy of Sciences Key Technology Talent Program ; Project of High-Level Talents Team Introduction in Zhuhai City ; Fundamental Research Funds for the Central Universities
WOS Research AreaRadiology, Nuclear Medicine & Medical Imaging
WOS SubjectRadiology, Nuclear Medicine & Medical Imaging
WOS IDWOS:000863801600001
PublisherSPRINGER
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Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/50368
Collection中国科学院分子影像重点实验室
Corresponding AuthorHui, Hui; Zhou, Bing; Tian, Jie
Affiliation1.Chinese Acad Sci, Inst Automat, Beijing Key Lab Mol Imaging, CAS Key Lab Mol Imaging,State Key Lab Management, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Beihang Univ, Sch Engn Med, Beijing 100191, Peoples R China
4.Beihang Univ, Minist Ind & Informat Technol, Key Lab Big Data Based Precis Med, Beijing 100191, Peoples R China
5.Jinan Univ, Zhuhai Peoples Hosp, Zhuhai Precis Med Ctr, Zhuhai 519000, Peoples R China
First Author AffilicationInstitute of Automation, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Automation, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Yueqi,Li, Changjian,Zhuo, Jiaming,et al. The Detection of Divalent Iron and Reactive Oxygen Species During Ferroptosis with the Use of a Dual-Reaction Turn-On Fluorescent Probe[J]. MOLECULAR IMAGING AND BIOLOGY,2022:12.
APA Wang, Yueqi,Li, Changjian,Zhuo, Jiaming,Hui, Hui,Zhou, Bing,&Tian, Jie.(2022).The Detection of Divalent Iron and Reactive Oxygen Species During Ferroptosis with the Use of a Dual-Reaction Turn-On Fluorescent Probe.MOLECULAR IMAGING AND BIOLOGY,12.
MLA Wang, Yueqi,et al."The Detection of Divalent Iron and Reactive Oxygen Species During Ferroptosis with the Use of a Dual-Reaction Turn-On Fluorescent Probe".MOLECULAR IMAGING AND BIOLOGY (2022):12.
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