First magnetic particle imaging to assess pulmonary vascular leakage in vivo in the acutely injured and fibrotic lung

doi:10.1002/btm2.10626

	First magnetic particle imaging to assess pulmonary vascular leakage in vivo in the acutely injured and fibrotic lung
	Feng, Xin1,2 ; Gao, Pengli 3,4,5,6; Li, Yabin 7; Hui, Hui1,2 ; Jiang, Jingying 5,6,9; Xie, Fei 7,10; Tian, Jie 1,5,6,8,9
发表期刊	BIOENGINEERING & TRANSLATIONAL MEDICINE
	2023-11-29
页码	10
通讯作者	Jiang, Jingying(jingyingjiang@buaa.edu.cn) ; Xie, Fei(xiefei0522@163.com) ; Tian, Jie(tian@ieee.org)
摘要	Increased pulmonary vascular permeability is a characteristic feature of lung injury. However, there are no established methods that allow the three-dimensional visualization and quantification of pulmonary vascular permeability in vivo. Evans blue extravasation test and total protein test of bronchoalveolar lavage fluid (BALF) are permeability assays commonly used in research settings. However, they lack the ability to identify the spatial and temporal heterogeneity of endothelial barrier disruption, which is typical in lung injuries. Magnetic resonance (MR) and near-infrared (NIR) imaging have been proposed to image pulmonary permeability, but suffer from limited sensitivity and penetration depth, respectively. In this study, we report the first use of magnetic particle imaging (MPI) to assess pulmonary vascular leakage noninvasively in vivo in mice. A dextran-coated superparamagnetic iron oxide (SPIO), synomag (R), was employed as the imaging tracer, and pulmonary SPIO extravasation was imaged and quantified to evaluate the vascular leakage. Animal models of acute lung injury and pulmonary fibrosis (PF) were used to validate the proposed method. MPI sensitively detected the SPIO extravasation in both acutely injured and fibrotic lungs in vivo, which was confirmed by ex vivo imaging and Prussian blue staining. Moreover, 3D MPI illustrated the spatial heterogeneity of vascular leakage, which correlated well with CT findings. Based on the in vivo 3D MPI images, we defined the SPIO extravasation index (SEI) to quantify the vascular leakage. A significant increase in SEI was observed in the injured lungs, in consistent with the results obtained via ex vivo permeability assays. Overall, our results demonstrate that 3D quantitative MPI serves as a useful tool to examine pulmonary vascular integrity in vivo, which shows promise for future clinical translation.
关键词	in vivo lung injury magnetic particle imaging quantitative vascular leakage
DOI	10.1002/btm2.10626
关键词[WOS]	MODELS
收录类别	SCI
语种	英语
资助项目	Natural Science Foundation of Beijing Municipality
项目资助者	Natural Science Foundation of Beijing Municipality
WOS研究方向	Biotechnology & Applied Microbiology ; Engineering ; Pharmacology & Pharmacy
WOS类目	Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Pharmacology & Pharmacy
WOS记录号	WOS:001115271700001
出版者	WILEY
引用统计
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/55102
专题	中国科学院分子影像重点实验室
通讯作者	Jiang, Jingying; Xie, Fei; Tian, Jie
作者单位	1.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing Key Lab Mol Imaging, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Sch Artificial Intelligence, Beijing, Peoples R China 3.Beihang Univ, Sch Biol Sci & Med Engn, Beijing, Peoples R China 4.Beihang Univ, Sch Engn Med, Beijing, Peoples R China 5.Beihang Univ, Key Lab Big Data Based Precis Med, Minist Ind & Informat Technol, Beijing, Peoples R China 6.Beihang Univ, Sch Engn Med, Beijing, Peoples R China 7.Chinese Peoples Liberat Army Gen Hosp, Coll Pulm & Crit Care Med, Beijing, Peoples R China 8.Beihang Univ, Sch Biol Sci & Med Engn, 37,Xueyuan Rd, Beijing 100191, Peoples R China 9.Beihang Univ, Sch Engn Med, 37,Xueyuan Rd, Beijing 100191, Peoples R China 10.Chinese Peoples Liberat Army Gen Hosp, Coll Pulm & Crit Care Med, 28 Fuxing Rd, Beijing 100853, Peoples R China
第一作者单位	中国科学院自动化研究所
通讯作者单位	中国科学院自动化研究所
推荐引用方式 GB/T 7714	Feng, Xin,Gao, Pengli,Li, Yabin,et al. First magnetic particle imaging to assess pulmonary vascular leakage in vivo in the acutely injured and fibrotic lung[J]. BIOENGINEERING & TRANSLATIONAL MEDICINE,2023:10.
APA	Feng, Xin.,Gao, Pengli.,Li, Yabin.,Hui, Hui.,Jiang, Jingying.,...&Tian, Jie.(2023).First magnetic particle imaging to assess pulmonary vascular leakage in vivo in the acutely injured and fibrotic lung.BIOENGINEERING & TRANSLATIONAL MEDICINE,10.
MLA	Feng, Xin,et al."First magnetic particle imaging to assess pulmonary vascular leakage in vivo in the acutely injured and fibrotic lung".BIOENGINEERING & TRANSLATIONAL MEDICINE (2023):10.