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Alternative TitleSystem Development and Algorithm Research on Optical Projection Tomography
Abstract自2002年光学投影断层成像(OPT)被提出以来,针对于1~10mm小模式样本的成像研究越来越多,这种分辨率高、无辐射、双模成像、成本低的新的成像模态逐渐得到了更多科研工作者的青睐和广泛研究,促进了该成像技术在基因表达、蛋白质相互作用和药物研发等领域的广泛应用。但是,国内在光学投影断层成像领域的发展比较缓慢,需要进一步进行成像系统和成像算法研究,结合更多的生物应用,来推动国内在光学投影断层成像技术方面的发展。 本文针对光学投影断层成像系统的开发和相关算法进行了研究,开发了第二代光学投影断层成像系统,并利用该系统进行了高密度成像和宽动态范围样本的成像研究。主要贡献包括: 1. 第二代光学投影断层成像系统的开发。开发了第二代光学投影断层成像系统,在系统分辨率、系统稳定性和系统便捷性方面进行硬件和软件开发。在光学原理基础上,开发了系统的硬件部分;在软件方面添加系统校正部分,方便实验中的调节。 2. 融合CT信息改善光学投影断层成像系统对高密度样本的成像效果。介绍了融合CT信息的光学投影断层成像方法,并探究了最佳成像时间。针对自身非透明的样本,采用透明化前的CT数据和透明化后的OPT数据的融合成像,并与单模态的CT成像、单模态的OPT成像对比,融合结果显示了更多的组织信息,验证了方法的成像效果。 3. 高动态多尺度融合和虚拟高动态的方法提高光学投影断层成像系统对宽动态范围样本的成像质量。介绍了高动态多尺度融合的OPT成像方法,采用亮环境和暗环境下的样本数据,通过切换不同的多尺度融合的方法来取得较好的重建效果。为减少对样本的数据采集次数,提出了虚拟高动态的光学投影断层成像方法,通过对采集数据分解为三个虚拟通道分别处理后再合成为最终的结果,提高了成像的视觉效果。
Other AbstractOptical projection tomography (OPT) was developed in 2002 as a mesoscopic scale optical imaging technique. After then more researches were focused on imaging specimens between 1mm and 10mm. This technique was rapidly applied to gene expression, fluorescent immunohistochemistry and phenotype worldwide because of the features of high-resolution, no radiation and low-cost. However, the development of OPT is growing relatively slow. Further investigation of imaging systems and algorithms combining biological applications is needed to promote the research field of OPT in our country. In this paper, we investigated imaging systems and algorithms related with OPT, and developed a second generation of OPT system. We did the experiments of high-density tissue imaging and wide dynamic range sample imaging. The main contributions include: 1. The 2nd OPT system is developed. By hardware and software development, the system has a better system resolution, stability and convenience. System calibration section added, the software makes the experiment adjustment easier. 2. CT/ OPT imaging is used for high density samples. The method of OPT combined CT information and their optimal imaging time are discussed. For non-transparent samples, CT data before BABB transparency and OPT data after BABB transparency are fused to improve the image quality of high density and soft tissues. And single mode CT imaging, single mode OPT imaging and CT/ OPT imaging are compared to verify the imaging method. 3. OPT imaging of multi scale integration and virtual HDR is used for imaging of wide range sample. A high dynamic multi scale fusion OPT method is designed by switching different multi scale fusion approach using light and dark environment’s sample data. And to reduce the numbers of data acquisition, virtual HDR is used to divide the original data and recombined to the final result to improve the visual imaging effect.
Keyword光学投影断层成像 系统开发 Ct融合 多尺度融合 虚拟高动态 Optical Projection Tomography System Development Ct Fusion Multi Scale Integration Virtual Hdr
Document Type学位论文
Recommended Citation
GB/T 7714
杨玉洁. 光学投影断层成像系统开发和算法研究[D]. 中国科学院自动化研究所. 中国科学院大学,2015.
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