Geometric control of capillary architecture via cell-matrix mechanical interactions
Sun, Jian1; Jamilpour, Nima1; Wang, Fei-Yue2; Wong, Pak Kin1
Source PublicationBIOMATERIALS
2014-03-01
Volume35Issue:10Pages:3273-3280
SubtypeArticle
AbstractCapillary morphogenesis is a multistage, multicellular activity that plays a pivotal role in various developmental and pathological situations. In-depth understanding of the regulatory mechanism along with the capability of controlling the morphogenic process will have direct implications on tissue engineering and therapeutic angiogenesis. Extensive research has been devoted to elucidate the biochemical factors that regulate capillary morphogenesis. The roles of geometric confinement and cell-matrix mechanical interactions on the capillary architecture, nevertheless, remain largely unknown. Here, we show geometric control of endothelial network topology by creating physical confinements with microfabricated fences and wells. Decreasing the thickness of the matrix also results in comparable modulation of the network architecture, supporting the boundary effect is mediated mechanically. The regulatory role of cell-matrix mechanical interaction on the network topology is further supported by alternating the matrix stiffness by a cell-inert PEG-dextran hydrogel. Furthermore, reducing the cell traction force with a Rho-associated protein kinase inhibitor diminishes the boundary effect. Computational biomechanical analysis delineates the relationship between geometric confinement and cell-matrix mechanical interaction. Collectively, these results reveal a mechanoregulation scheme of endothelial cells to regulate the capillary network architecture via cell-matrix mechanical interactions. (C) 2014 Elsevier Ltd. All rights reserved.
KeywordGeometric Constraints Capillary Morphogenesis Cell-matrix Interactions Hydrogel Angiogenesis
WOS HeadingsScience & Technology ; Technology
WOS KeywordATOMIC-FORCE MICROSCOPY ; IN-VITRO ; ENDOTHELIAL-CELLS ; TISSUE ; ANGIOGENESIS ; SUBSTRATE ; MORPHOGENESIS ; STIFFNESS ; NETWORKS ; FEEL
Indexed BySCI
Language英语
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000332188400014
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/8026
Collection复杂系统管理与控制国家重点实验室_先进控制与自动化
Affiliation1.Univ Arizona, Dept Aerosp & Mech Engn, Tucson, AZ 85721 USA
2.Chinese Acad Sci, Inst Automat, Key Lab Complex Syst & Intelligence Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Sun, Jian,Jamilpour, Nima,Wang, Fei-Yue,et al. Geometric control of capillary architecture via cell-matrix mechanical interactions[J]. BIOMATERIALS,2014,35(10):3273-3280.
APA Sun, Jian,Jamilpour, Nima,Wang, Fei-Yue,&Wong, Pak Kin.(2014).Geometric control of capillary architecture via cell-matrix mechanical interactions.BIOMATERIALS,35(10),3273-3280.
MLA Sun, Jian,et al."Geometric control of capillary architecture via cell-matrix mechanical interactions".BIOMATERIALS 35.10(2014):3273-3280.
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