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Scalable and Dil-compatible optical clearance of the mammalian brain
Hou, Bing1,2,3; Zhang, Dan1; Zhao, Shan1; Wei, Mengping4,5; Yang, Zaifu3; Wang, Shaoxia3; Wang, Jiarui3; Zhang, Xin1; Liu, Bing1,2; Fan, Lingzhong1; Li, Yang3; Qiu, Zilong6; Zhang, Chen4,5; Jiang, Tianzi1,2,7,8,9
AbstractEfficient optical clearance is fundamental for whole brain imaging. In particular, clearance of the brain without membrane damage is required for the imaging of lipophilic tracer-labeled neural tracts. Relying on an ascending gradient of fructose solutions, SeeDB can achieve sufficient transparency of the mouse brain while ensuring that the plasma membrane remains intact. However, it is challenging to extend this method to larger mammalian brains due to the extremely high viscosity of the saturated fructose solution. Here we report a SeeDB-derived optical clearing method, termed FRUIT which utilizes a cocktail of fructose and urea. As demonstrated in the adult mouse brain, combination of these two highly water-soluble clearing agents exerts a synergistic effect on clearance. More importantly, the final FRUIT solution has low viscosity so as to produce transparency of the whole adult rabbit brain via arterial perfusion, which is impossible to achieve with a saturated fructose solution. In addition to good compatibility with enhanced yellow fluorescent protein, the cocktail also preserves the fluorescence of the lipophilic tracer Dil. This work provides a volume-independent optical clearing method which retains the advantages of SeeDB, particularly compatibility with lipophilic tracers.
KeywordOptical Tissue Clearing Seedb Urea Tract Tracing Whole Brain Imaging Clarity Cubic 3disco
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
WOS Research AreaAnatomy & Morphology ; Neurosciences & Neurology
WOS SubjectAnatomy & Morphology ; Neurosciences
WOS IDWOS:000352568100001
Citation statistics
Cited Times:47[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Inst Automat, Brainnetome Ctr, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Automat, Natl Lab Pattern Recognit, Beijing 100190, Peoples R China
3.Beijing Inst Radiat Med, Beijing, Peoples R China
4.Peking Univ, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China
5.Peking Univ, PKU IDG McGovem Inst Brain Res, Sch Life Sci, Beijing 100871, Peoples R China
6.Shanghai Inst Biol Sci, Inst Neurosci, Shanghai, Peoples R China
7.Univ Queensland, Queensland Brain Inst, Brisbane, Qld, Australia
8.Univ Elect Sci & Technol China, Sch Life Sci & Technol, Minist Educ, Key Lab NeuroInformat, Chengdu 610054, Peoples R China
9.Chinese Acad Sci, Inst Automat, CAS Ctr Excellence Brain Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Hou, Bing,Zhang, Dan,Zhao, Shan,et al. Scalable and Dil-compatible optical clearance of the mammalian brain[J]. FRONTIERS IN NEUROANATOMY,2015,9.
APA Hou, Bing.,Zhang, Dan.,Zhao, Shan.,Wei, Mengping.,Yang, Zaifu.,...&Jiang, Tianzi.(2015).Scalable and Dil-compatible optical clearance of the mammalian brain.FRONTIERS IN NEUROANATOMY,9.
MLA Hou, Bing,et al."Scalable and Dil-compatible optical clearance of the mammalian brain".FRONTIERS IN NEUROANATOMY 9(2015).
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