CASIA OpenIR  > 中国科学院分子影像重点实验室
Increased precision of orthotopic and metastatic breast cancer surgery guided by matrix metalloproteinase-activatable near-infrared fluorescence probes
Chi, Chongwei1; Zhang, Qian2; Mao, Yamin1; Kou, Deqiang3; Qiu, Jingdan3; Ye, Jinzuo1; Wang, Jiandong3; Wang, Zhongliang2; Du, Yang1; Tian, Jie1
Source PublicationSCIENTIFIC REPORTS
2015-09-23
Volume5Pages:1-10
SubtypeArticle
AbstractAdvanced medical imaging technology has allowed the use of fluorescence molecular imaging-guided breast cancer surgery (FMI-guided BCS) to specifically label tumour cells and to precisely distinguish tumour margins from normal tissues intra-operatively, a major challenge in the medical field. Here, we developed a surgical navigation system for real-time FMI-guided BCS. Tumours derived from highly metastatic 4T1-luc breast cancer cells, which exhibit high expression of matrix metalloproteinase (MMP) and human epidermal growth factor receptor 2 (HER2), were established in nude mice; these mice were injected with smart MMP-targeting and "always-on" HER2-targeting near-infrared (NIR) fluorescent probes. The fluorescence signal was imaged to assess in vivo binding of the probes to the tumour and metastatic sites. Then, orthotopic and metastatic breast tumours were precisely removed under the guidance of our system. The post-operative survival rate of mice was improved by 50% with the new method. Hematoxylin and eosin staining and immunohistochemical staining for MMP2 and CD11b further confirmed the precision of tumour dissection. Our method facilitated the accurate detection and complete removal of breast cancer tumours and provided a method for defining the molecular classification of breast cancer during surgery, thereby improving prognoses and survival rates.
WOS HeadingsScience & Technology
DOI10.1038/srep14197
WOS KeywordCELL-PENETRATING PEPTIDES ; PANCREATIC-CANCER ; IN-VIVO ; SURVIVAL ; FLUOROPHORES ; PROTOTYPE ; MARGINS ; TUMORS
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000361591900001
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/9020
Collection中国科学院分子影像重点实验室
Affiliation1.Chinese Acad Sci, Inst Automat, Key Lab Mol Imaging, Beijing 100190, Peoples R China
2.Xidian Univ, Sch Life Sci & Technol, Xian 710071, Peoples R China
3.Peoples Liberat Army, Gen Hosp, Dept Gen Surg, Beijing 100853, Peoples R China
Recommended Citation
GB/T 7714
Chi, Chongwei,Zhang, Qian,Mao, Yamin,et al. Increased precision of orthotopic and metastatic breast cancer surgery guided by matrix metalloproteinase-activatable near-infrared fluorescence probes[J]. SCIENTIFIC REPORTS,2015,5:1-10.
APA Chi, Chongwei.,Zhang, Qian.,Mao, Yamin.,Kou, Deqiang.,Qiu, Jingdan.,...&Tian, Jie.(2015).Increased precision of orthotopic and metastatic breast cancer surgery guided by matrix metalloproteinase-activatable near-infrared fluorescence probes.SCIENTIFIC REPORTS,5,1-10.
MLA Chi, Chongwei,et al."Increased precision of orthotopic and metastatic breast cancer surgery guided by matrix metalloproteinase-activatable near-infrared fluorescence probes".SCIENTIFIC REPORTS 5(2015):1-10.
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