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Increased precision of orthotopic and metastatic breast cancer surgery guided by matrix metalloproteinase-activatable near-infrared fluorescence probes
Chi, Chongwei1; Zhang, Qian2; Mao, Yamin1; Kou, Deqiang3; Qiu, Jingdan3; Ye, Jinzuo1; Wang, Jiandong3; Wang, Zhongliang2; Du, Yang1; Tian, Jie1
2015-09-23
发表期刊SCIENTIFIC REPORTS
卷号5页码:1-10
文章类型Article
摘要Advanced medical imaging technology has allowed the use of fluorescence molecular imaging-guided breast cancer surgery (FMI-guided BCS) to specifically label tumour cells and to precisely distinguish tumour margins from normal tissues intra-operatively, a major challenge in the medical field. Here, we developed a surgical navigation system for real-time FMI-guided BCS. Tumours derived from highly metastatic 4T1-luc breast cancer cells, which exhibit high expression of matrix metalloproteinase (MMP) and human epidermal growth factor receptor 2 (HER2), were established in nude mice; these mice were injected with smart MMP-targeting and "always-on" HER2-targeting near-infrared (NIR) fluorescent probes. The fluorescence signal was imaged to assess in vivo binding of the probes to the tumour and metastatic sites. Then, orthotopic and metastatic breast tumours were precisely removed under the guidance of our system. The post-operative survival rate of mice was improved by 50% with the new method. Hematoxylin and eosin staining and immunohistochemical staining for MMP2 and CD11b further confirmed the precision of tumour dissection. Our method facilitated the accurate detection and complete removal of breast cancer tumours and provided a method for defining the molecular classification of breast cancer during surgery, thereby improving prognoses and survival rates.
WOS标题词Science & Technology
DOI10.1038/srep14197
关键词[WOS]CELL-PENETRATING PEPTIDES ; PANCREATIC-CANCER ; IN-VIVO ; SURVIVAL ; FLUOROPHORES ; PROTOTYPE ; MARGINS ; TUMORS
收录类别SCI
语种英语
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000361591900001
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ia.ac.cn/handle/173211/9020
专题中国科学院分子影像重点实验室
作者单位1.Chinese Acad Sci, Inst Automat, Key Lab Mol Imaging, Beijing 100190, Peoples R China
2.Xidian Univ, Sch Life Sci & Technol, Xian 710071, Peoples R China
3.Peoples Liberat Army, Gen Hosp, Dept Gen Surg, Beijing 100853, Peoples R China
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Chi, Chongwei,Zhang, Qian,Mao, Yamin,et al. Increased precision of orthotopic and metastatic breast cancer surgery guided by matrix metalloproteinase-activatable near-infrared fluorescence probes[J]. SCIENTIFIC REPORTS,2015,5:1-10.
APA Chi, Chongwei.,Zhang, Qian.,Mao, Yamin.,Kou, Deqiang.,Qiu, Jingdan.,...&Tian, Jie.(2015).Increased precision of orthotopic and metastatic breast cancer surgery guided by matrix metalloproteinase-activatable near-infrared fluorescence probes.SCIENTIFIC REPORTS,5,1-10.
MLA Chi, Chongwei,et al."Increased precision of orthotopic and metastatic breast cancer surgery guided by matrix metalloproteinase-activatable near-infrared fluorescence probes".SCIENTIFIC REPORTS 5(2015):1-10.
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