CASIA OpenIR  > 09年以前成果
AMASS: Software for automatically validating the quality of MS/MS spectrum from SEQUEST results
Sun, W; Li, FX; Wang, J; Zheng, DX; Gao, YH
Source PublicationMOLECULAR & CELLULAR PROTEOMICS
2004-12-01
Volume3Issue:12Pages:1194-1199
SubtypeArticle
AbstractTime-consuming and experience-dependent manual validations of tandem mass spectra are usually applied to SEQUEST results. This inefficient method has become a significant bottleneck for MS/MS data processing. Here we introduce a program AMASS ( advanced mass spectrum screener), which can filter the tandem mass spectra of SEQUEST results by measuring the match percentage of high-abundant ions and the continuity of matched fragment ions in b, y series. Compared with Xcorr and DeltaCn filter, AMASS can increase the number of positives and reduce the number of negatives in 22 datasets generated from 18 known protein mixtures. It effectively removed most noisy spectra, false interpretations, and about half of poor fragmentation spectra, and AMASS can work synergistically with Rscore filter. We believe the use of AMASS and Rscore can result in a more accurate identification of peptide MS/MS spectra and reduce the time and energy for manual validation.
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS KeywordTANDEM MASS-SPECTROMETRY ; PROTEIN IDENTIFICATION ; YEAST PROTEOME ; STATISTICAL-MODEL ; DATABASE SEARCH ; COMPLEXES ; ALGORITHM
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000226100700005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/9043
Collection09年以前成果
Affiliation1.Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Proteom Res Ctr, Beijing 100005, Peoples R China
2.Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Natl Key Lab Med Mol Biol, Beijing 100005, Peoples R China
3.Chinese Acad Sci, Inst Automat, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Sun, W,Li, FX,Wang, J,et al. AMASS: Software for automatically validating the quality of MS/MS spectrum from SEQUEST results[J]. MOLECULAR & CELLULAR PROTEOMICS,2004,3(12):1194-1199.
APA Sun, W,Li, FX,Wang, J,Zheng, DX,&Gao, YH.(2004).AMASS: Software for automatically validating the quality of MS/MS spectrum from SEQUEST results.MOLECULAR & CELLULAR PROTEOMICS,3(12),1194-1199.
MLA Sun, W,et al."AMASS: Software for automatically validating the quality of MS/MS spectrum from SEQUEST results".MOLECULAR & CELLULAR PROTEOMICS 3.12(2004):1194-1199.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sun, W]'s Articles
[Li, FX]'s Articles
[Wang, J]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sun, W]'s Articles
[Li, FX]'s Articles
[Wang, J]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sun, W]'s Articles
[Li, FX]'s Articles
[Wang, J]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.