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AMASS: Software for automatically validating the quality of MS/MS spectrum from SEQUEST results
Sun, W; Li, FX; Wang, J; Zheng, DX; Gao, YH
2004-12-01
发表期刊MOLECULAR & CELLULAR PROTEOMICS
卷号3期号:12页码:1194-1199
文章类型Article
摘要Time-consuming and experience-dependent manual validations of tandem mass spectra are usually applied to SEQUEST results. This inefficient method has become a significant bottleneck for MS/MS data processing. Here we introduce a program AMASS ( advanced mass spectrum screener), which can filter the tandem mass spectra of SEQUEST results by measuring the match percentage of high-abundant ions and the continuity of matched fragment ions in b, y series. Compared with Xcorr and DeltaCn filter, AMASS can increase the number of positives and reduce the number of negatives in 22 datasets generated from 18 known protein mixtures. It effectively removed most noisy spectra, false interpretations, and about half of poor fragmentation spectra, and AMASS can work synergistically with Rscore filter. We believe the use of AMASS and Rscore can result in a more accurate identification of peptide MS/MS spectra and reduce the time and energy for manual validation.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]TANDEM MASS-SPECTROMETRY ; PROTEIN IDENTIFICATION ; YEAST PROTEOME ; STATISTICAL-MODEL ; DATABASE SEARCH ; COMPLEXES ; ALGORITHM
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemical Research Methods
WOS记录号WOS:000226100700005
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被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ia.ac.cn/handle/173211/9043
专题09年以前成果
作者单位1.Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Proteom Res Ctr, Beijing 100005, Peoples R China
2.Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Natl Key Lab Med Mol Biol, Beijing 100005, Peoples R China
3.Chinese Acad Sci, Inst Automat, Beijing 100080, Peoples R China
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Sun, W,Li, FX,Wang, J,et al. AMASS: Software for automatically validating the quality of MS/MS spectrum from SEQUEST results[J]. MOLECULAR & CELLULAR PROTEOMICS,2004,3(12):1194-1199.
APA Sun, W,Li, FX,Wang, J,Zheng, DX,&Gao, YH.(2004).AMASS: Software for automatically validating the quality of MS/MS spectrum from SEQUEST results.MOLECULAR & CELLULAR PROTEOMICS,3(12),1194-1199.
MLA Sun, W,et al."AMASS: Software for automatically validating the quality of MS/MS spectrum from SEQUEST results".MOLECULAR & CELLULAR PROTEOMICS 3.12(2004):1194-1199.
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