Dynamic urinary proteomic analysis reveals stable proteins to be potential biomarkers
Sun, Wei1; Chen, Yong2; Li, Fuxin3; Zhang, Ling4; Yang, Ruifeng5; Zhang, Zhi5; Zheng, Dexian1; Gao, Youhe1
Source PublicationPROTEOMICS CLINICAL APPLICATIONS
2009-03-01
Volume3Issue:3Pages:370-382
SubtypeArticle
AbstractHuman urinary proteome analysis is a convenient and efficient approach for understanding disease processes affecting the kidney and urogenital tract. Many potential biomarkers have been identified in previous differential analyses; however, dynamic variations of the urinary proteome have not been intensively studied, and it is difficult to conclude that potential biomarkers are genuinely associated with disease rather then simply being physiological proteome variations. In this paper, pooled and individual urine samples were used to analyze dynamic variations in the urinary proteome. Five types of pooled samples (first morning void, second morning void, excessive water-drinking void, random void, and 24 h void) collected in 1 day from six volunteers were used to analyze intra-day variations. Six pairs of first morning voids collected a week apart were used to study inter-day, inter-individual, and inter-gender variations. The intra-day, inter-day, inter-individual, and inter-gender variation analyses showed that many proteins were constantly present with relatively stable abundances, and some of these had earlier been reported as potential disease biomarkers. In terms of sensitivity, the main components of the five intra-day urinary proteomes were similar, and the second morning void is recommended for clinical proteome analysis. The advantages and disadvantages of pooling samples are also discussed. The data presented describe a pool of stable urinary proteins seen under different physiological conditions. Any significant qualitative or quantitative changes in these stable proteins may mean that such proteins could serve as potential urinary biomarkers.
KeywordBiomarker Dynamic Analysis Stable Protein Urinary Proteome
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS KeywordTANDEM MASS-SPECTROMETRY ; PROSTAGLANDIN-D-SYNTHASE ; TAMM-HORSFALL GLYCOPROTEIN ; LIQUID-CHROMATOGRAPHY ; CEREBROSPINAL-FLUID ; BETA-TRACE ; SAMPLE PREPARATION ; POPULATION ; DISCOVERY ; CANCER
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS IDWOS:000264794700008
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Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/9975
Collection复杂系统管理与控制国家重点实验室_先进控制与自动化
Affiliation1.Chinese Acad Med Sci, Peking Union Med Coll, Dept Physiol & Pathophysiol, Inst Basic Med Sci,Sch Basic Med,Prote Res Ctr, Beijing 100005, Peoples R China
2.Lanzhou Univ, Lanzhou Inst Biol Prod, China Natl Biotech Grp, Lanzhou 730000, Gansu, Peoples R China
3.Chinese Acad Sci, Inst Automat, Beijing, Peoples R China
4.Chinese Natl Inst Metrol, Beijing, Peoples R China
5.Chinese Acad Med Sci, Peking Union Med Coll, Dept Biomed Engn, Inst Basic Med Sci,Sch Basic Med, Beijing 100005, Peoples R China
Recommended Citation
GB/T 7714
Sun, Wei,Chen, Yong,Li, Fuxin,et al. Dynamic urinary proteomic analysis reveals stable proteins to be potential biomarkers[J]. PROTEOMICS CLINICAL APPLICATIONS,2009,3(3):370-382.
APA Sun, Wei.,Chen, Yong.,Li, Fuxin.,Zhang, Ling.,Yang, Ruifeng.,...&Gao, Youhe.(2009).Dynamic urinary proteomic analysis reveals stable proteins to be potential biomarkers.PROTEOMICS CLINICAL APPLICATIONS,3(3),370-382.
MLA Sun, Wei,et al."Dynamic urinary proteomic analysis reveals stable proteins to be potential biomarkers".PROTEOMICS CLINICAL APPLICATIONS 3.3(2009):370-382.
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