Penetrating Peptide-Bioconjugated Persistent Nanophosphors for Long-Term Tracking of Adipose-Derived Stem Cells with Superior Signal-to-Noise Ratio

doi:10.1021/acs.analchem.6b00449

	Penetrating Peptide-Bioconjugated Persistent Nanophosphors for Long-Term Tracking of Adipose-Derived Stem Cells with Superior Signal-to-Noise Ratio
	Wu, Shu-Qi 1; Chi, Chong-Wei3 ; Yang, Cheng-Xiong 1; Yan, Xiu-Ping 1,2
发表期刊	ANALYTICAL CHEMISTRY
	2016-04-05
卷号	88 期号:7 页码:4114-4121
文章类型	Article
摘要	Reliable long-term in vivo tracking of stem cells is of great importance in stem cell-based therapy and research. Fluorescence imaging with in situ excitation has significant autofluorescence background, which results in poor signal-to-noise ratio (SNR). Here we report TAT penetrating peptide-bioconjugated long persistent luminescence nanoparticles (LPLNP-TAT) for long-term tracking of adipose-derived stem cells (ASC) without constant external excitation. LPLNP-TAT exhibits near-infrared emitting, red light renewable capability, and superior in vivo imaging depth and SNR compared with conventional organic dye and quantum dots. Our findings show that LPLNP-TAT can successfully label ASC without impairing their proliferation and differentiation and can effectively track ASC in skin-regeneration and tumor-homing models. We believe that LPLNP-TAT represents a new generation of cell tracking probes and will have broad application in diagnosis and therapy.
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1021/acs.analchem.6b00449
关键词[WOS]	UP-CONVERSION NANOPARTICLES ; REGENERATIVE MEDICINE ; LUMINESCENT NANOPROBES ; THERAPY ; ENGRAFTMENT ; NANODOTS ; DELIVERY ; LIFETIME
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21435001 ; National Basic Research Program of China(2011CB707703) ; 21275079 ; 81227901 ; 61501462)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000373656300092
引用统计	被引频次：81[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/11318
专题	中国科学院分子影像重点实验室
作者单位	1.Nankai Univ, State Key Lab Med Chem Biol, Res Ctr Analyt Sci, Coll Chem, Tianjin 300071, Peoples R China 2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Inst Automat, Key Lab Mol Imaging, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Wu, Shu-Qi,Chi, Chong-Wei,Yang, Cheng-Xiong,et al. Penetrating Peptide-Bioconjugated Persistent Nanophosphors for Long-Term Tracking of Adipose-Derived Stem Cells with Superior Signal-to-Noise Ratio[J]. ANALYTICAL CHEMISTRY,2016,88(7):4114-4121.
APA	Wu, Shu-Qi,Chi, Chong-Wei,Yang, Cheng-Xiong,&Yan, Xiu-Ping.(2016).Penetrating Peptide-Bioconjugated Persistent Nanophosphors for Long-Term Tracking of Adipose-Derived Stem Cells with Superior Signal-to-Noise Ratio.ANALYTICAL CHEMISTRY,88(7),4114-4121.
MLA	Wu, Shu-Qi,et al."Penetrating Peptide-Bioconjugated Persistent Nanophosphors for Long-Term Tracking of Adipose-Derived Stem Cells with Superior Signal-to-Noise Ratio".ANALYTICAL CHEMISTRY 88.7(2016):4114-4121.