Three-dimensional light field microscope based on a lenslet array

doi:10.1016/j.optcom.2017.07.026

CASIA OpenIR > 多模态人工智能系统全国重点实验室 > 平行智能技术与系统团队

	Three-dimensional light field microscope based on a lenslet array
	Zhang, Mei; Geng, Zheng; Pei, Renjing; Cao, Xuan; Zhang, Zhaoxing
发表期刊	OPTICS COMMUNICATIONS
	2017-11-15
期号	403 页码:133-142
文章类型	Article
摘要	Most conventional microscopes can only acquire two-dimensional (2D) images. However, there is increasing demand for microscopes capable of acquiring three-dimensional (3D) images for use in biological research. To improve observations of in vivo specimens, we develop a novel one snap-shot 3D light field microscope with high spatial resolution. By adding a lenslet array and focusing optics into the optical train of a conventional microscope, we are able to simultaneously capture seven sub-aperture images from different perspectives in a single shot, and finish 3D surface reconstruction of the specimen. In this paper, we present a functioning prototype of a novel 3D light field microscope using different objectives, and describe its design and image quality. During 3D image reconstruction, we use an optical flow method and back-projection interpolation to improve the resolution of the subimages. The performance of the novel light field microscope is analyzed theoretically and experimentally. Multiple perspective images are obtained using an objective with 20 x /NA 0.4. These images are 1050 x 1050 pixels with a depth of view of 200 mu m. For our microscope, the highest resolvable group of USAF 1951 target is about group 7.5. 3D reconstructions of various specimens are successfully created using these images. This novel microscopy system is suitable for various applications in 3D imaging and optical metrology. (C) 2017 Elsevier B.V. All rights reserved.
关键词	Light Field Three-dimensional Microscopy High Resolution 3d Image Acquisition 3d Image Reconstruction
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1016/j.optcom.2017.07.026
关键词[WOS]	ILLUMINATION MICROSCOPY ; NEURONAL-ACTIVITY
收录类别	SCI
语种	英语
项目资助者	863 Program(2015AA015905) ; National Natural Science Foundation of China (NSFC)(61605240)
WOS研究方向	Optics
WOS类目	Optics
WOS记录号	WOS:000410638900021
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/20728
专题	多模态人工智能系统全国重点实验室_平行智能技术与系统团队
作者单位	Chinese Acad Sci, Inst Automat, State Key Lab Management & Control Complex Syst, Beijing, Peoples R China
第一作者单位	中国科学院自动化研究所
推荐引用方式 GB/T 7714	Zhang, Mei,Geng, Zheng,Pei, Renjing,et al. Three-dimensional light field microscope based on a lenslet array[J]. OPTICS COMMUNICATIONS,2017(403):133-142.
APA	Zhang, Mei,Geng, Zheng,Pei, Renjing,Cao, Xuan,&Zhang, Zhaoxing.(2017).Three-dimensional light field microscope based on a lenslet array.OPTICS COMMUNICATIONS(403),133-142.
MLA	Zhang, Mei,et al."Three-dimensional light field microscope based on a lenslet array".OPTICS COMMUNICATIONS .403(2017):133-142.

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