Three-dimensional light field microscope based on a lenslet array
Zhang, Mei; Geng, Zheng; Pei, Renjing; Cao, Xuan; Zhang, Zhaoxing
AbstractMost conventional microscopes can only acquire two-dimensional (2D) images. However, there is increasing demand for microscopes capable of acquiring three-dimensional (3D) images for use in biological research. To improve observations of in vivo specimens, we develop a novel one snap-shot 3D light field microscope with high spatial resolution. By adding a lenslet array and focusing optics into the optical train of a conventional microscope, we are able to simultaneously capture seven sub-aperture images from different perspectives in a single shot, and finish 3D surface reconstruction of the specimen. In this paper, we present a functioning prototype of a novel 3D light field microscope using different objectives, and describe its design and image quality. During 3D image reconstruction, we use an optical flow method and back-projection interpolation to improve the resolution of the subimages. The performance of the novel light field microscope is analyzed theoretically and experimentally. Multiple perspective images are obtained using an objective with 20 x /NA 0.4. These images are 1050 x 1050 pixels with a depth of view of 200 mu m. For our microscope, the highest resolvable group of USAF 1951 target is about group 7.5. 3D reconstructions of various specimens are successfully created using these images. This novel microscopy system is suitable for various applications in 3D imaging and optical metrology. (C) 2017 Elsevier B.V. All rights reserved.
KeywordLight Field Three-dimensional Microscopy High Resolution 3d Image Acquisition 3d Image Reconstruction
WOS HeadingsScience & Technology ; Physical Sciences
Indexed BySCI
Funding Organization863 Program(2015AA015905) ; National Natural Science Foundation of China (NSFC)(61605240)
WOS Research AreaOptics
WOS SubjectOptics
WOS IDWOS:000410638900021
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
AffiliationChinese Acad Sci, Inst Automat, State Key Lab Management & Control Complex Syst, Beijing, Peoples R China
First Author AffilicationInstitute of Automation, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Mei,Geng, Zheng,Pei, Renjing,et al. Three-dimensional light field microscope based on a lenslet array[J]. OPTICS COMMUNICATIONS,2017(403):133-142.
APA Zhang, Mei,Geng, Zheng,Pei, Renjing,Cao, Xuan,&Zhang, Zhaoxing.(2017).Three-dimensional light field microscope based on a lenslet array.OPTICS COMMUNICATIONS(403),133-142.
MLA Zhang, Mei,et al."Three-dimensional light field microscope based on a lenslet array".OPTICS COMMUNICATIONS .403(2017):133-142.
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