Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects

doi:10.1016/j.omtn.2018.01.008

	Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects
	Zheng, Haifeng 1; Zhou, Lin 1; Shi, Yaru 1; Tian, Jie 1,2; Wang, Fu 1
发表期刊	MOLECULAR THERAPY-NUCLEIC ACIDS
	2018-06-01
卷号	11 页码:34-40
文章类型	Article
摘要	MicroRNAs (miRNAs) are a class of negative regulators of gene expression and play critical roles in various biological processes. Conventional approaches for detecting miRNAs, such as northern blotting, microarray, and real-time PCR, usually require the lysis of cell samples and could not provide the in vivo information about miRNAs in living organisms. Here, we designed a tyrosinase (TYR)-based reporter to monitor miR-9 expression that is regulated by DNA methylation in living cells and animals. During DNA methylation of A549 cells treated by 5-aza-2'-deoxycytidine (5-Aza-dC), the CMV/TYR-3xTS reporter-transfected cells demonstrated a gradual decrease in melanin content, TYR activity, and photoacoustic signal because of the gradual activation of miR-9 expression. The miR-9-regulated repression of TYR activity also resulted in a significant decrease in photoacoustic signal from the flank of mice with 5-Aza-dC treatment, whereas the bioluminescence signal from internal control had no obvious change. The TYR-based miRNA reporter may serve as a new imaging probe for monitoring the dynamic expression of miRNAs during various cellular or disease progression in cells and living animals.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.omtn.2018.01.008
关键词[WOS]	IN-VIVO ; HUMAN CANCER ; HYPERMETHYLATION ; MICROSCOPY ; BIOGENESIS ; TARGETS ; SYSTEM
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(81571721 ; Natural Science Basis Research Plan in Shaanxi Province of China(2016JM8016) ; 81772010)
WOS研究方向	Research & Experimental Medicine
WOS类目	Medicine, Research & Experimental
WOS记录号	WOS:000433428900004
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/22047
专题	中国科学院分子影像重点实验室
作者单位	1.Xidian Univ, Sch Life Sci & Technol, Engn Res Ctr Mol & Neuro Imaging, Minist Educ, Xian 710071, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Haifeng,Zhou, Lin,Shi, Yaru,et al. Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects[J]. MOLECULAR THERAPY-NUCLEIC ACIDS,2018,11:34-40.
APA	Zheng, Haifeng,Zhou, Lin,Shi, Yaru,Tian, Jie,&Wang, Fu.(2018).Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects.MOLECULAR THERAPY-NUCLEIC ACIDS,11,34-40.
MLA	Zheng, Haifeng,et al."Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects".MOLECULAR THERAPY-NUCLEIC ACIDS 11(2018):34-40.