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Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects
Zheng, Haifeng1; Zhou, Lin1; Shi, Yaru1; Tian, Jie1,2; Wang, Fu1
2018-06-01
发表期刊MOLECULAR THERAPY-NUCLEIC ACIDS
卷号11页码:34-40
文章类型Article
摘要MicroRNAs (miRNAs) are a class of negative regulators of gene expression and play critical roles in various biological processes. Conventional approaches for detecting miRNAs, such as northern blotting, microarray, and real-time PCR, usually require the lysis of cell samples and could not provide the in vivo information about miRNAs in living organisms. Here, we designed a tyrosinase (TYR)-based reporter to monitor miR-9 expression that is regulated by DNA methylation in living cells and animals. During DNA methylation of A549 cells treated by 5-aza-2'-deoxycytidine (5-Aza-dC), the CMV/TYR-3xTS reporter-transfected cells demonstrated a gradual decrease in melanin content, TYR activity, and photoacoustic signal because of the gradual activation of miR-9 expression. The miR-9-regulated repression of TYR activity also resulted in a significant decrease in photoacoustic signal from the flank of mice with 5-Aza-dC treatment, whereas the bioluminescence signal from internal control had no obvious change. The TYR-based miRNA reporter may serve as a new imaging probe for monitoring the dynamic expression of miRNAs during various cellular or disease progression in cells and living animals.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.omtn.2018.01.008
关键词[WOS]IN-VIVO ; HUMAN CANCER ; HYPERMETHYLATION ; MICROSCOPY ; BIOGENESIS ; TARGETS ; SYSTEM
收录类别SCI
语种英语
项目资助者National Natural Science Foundation of China(81571721 ; Natural Science Basis Research Plan in Shaanxi Province of China(2016JM8016) ; 81772010)
WOS研究方向Research & Experimental Medicine
WOS类目Medicine, Research & Experimental
WOS记录号WOS:000433428900004
引用统计
文献类型期刊论文
条目标识符http://ir.ia.ac.cn/handle/173211/22047
专题中国科学院分子影像重点实验室
作者单位1.Xidian Univ, Sch Life Sci & Technol, Engn Res Ctr Mol & Neuro Imaging, Minist Educ, Xian 710071, Shaanxi, Peoples R China
2.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
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Zheng, Haifeng,Zhou, Lin,Shi, Yaru,et al. Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects[J]. MOLECULAR THERAPY-NUCLEIC ACIDS,2018,11:34-40.
APA Zheng, Haifeng,Zhou, Lin,Shi, Yaru,Tian, Jie,&Wang, Fu.(2018).Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects.MOLECULAR THERAPY-NUCLEIC ACIDS,11,34-40.
MLA Zheng, Haifeng,et al."Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects".MOLECULAR THERAPY-NUCLEIC ACIDS 11(2018):34-40.
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