Institutional Repository of Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects | |
Zheng, Haifeng1; Zhou, Lin1; Shi, Yaru1; Tian, Jie1,2; Wang, Fu1 | |
发表期刊 | MOLECULAR THERAPY-NUCLEIC ACIDS
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2018-06-01 | |
卷号 | 11页码:34-40 |
文章类型 | Article |
摘要 | MicroRNAs (miRNAs) are a class of negative regulators of gene expression and play critical roles in various biological processes. Conventional approaches for detecting miRNAs, such as northern blotting, microarray, and real-time PCR, usually require the lysis of cell samples and could not provide the in vivo information about miRNAs in living organisms. Here, we designed a tyrosinase (TYR)-based reporter to monitor miR-9 expression that is regulated by DNA methylation in living cells and animals. During DNA methylation of A549 cells treated by 5-aza-2'-deoxycytidine (5-Aza-dC), the CMV/TYR-3xTS reporter-transfected cells demonstrated a gradual decrease in melanin content, TYR activity, and photoacoustic signal because of the gradual activation of miR-9 expression. The miR-9-regulated repression of TYR activity also resulted in a significant decrease in photoacoustic signal from the flank of mice with 5-Aza-dC treatment, whereas the bioluminescence signal from internal control had no obvious change. The TYR-based miRNA reporter may serve as a new imaging probe for monitoring the dynamic expression of miRNAs during various cellular or disease progression in cells and living animals. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1016/j.omtn.2018.01.008 |
关键词[WOS] | IN-VIVO ; HUMAN CANCER ; HYPERMETHYLATION ; MICROSCOPY ; BIOGENESIS ; TARGETS ; SYSTEM |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China(81571721 ; Natural Science Basis Research Plan in Shaanxi Province of China(2016JM8016) ; 81772010) |
WOS研究方向 | Research & Experimental Medicine |
WOS类目 | Medicine, Research & Experimental |
WOS记录号 | WOS:000433428900004 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ia.ac.cn/handle/173211/22047 |
专题 | 中国科学院分子影像重点实验室 |
作者单位 | 1.Xidian Univ, Sch Life Sci & Technol, Engn Res Ctr Mol & Neuro Imaging, Minist Educ, Xian 710071, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Zheng, Haifeng,Zhou, Lin,Shi, Yaru,et al. Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects[J]. MOLECULAR THERAPY-NUCLEIC ACIDS,2018,11:34-40. |
APA | Zheng, Haifeng,Zhou, Lin,Shi, Yaru,Tian, Jie,&Wang, Fu.(2018).Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects.MOLECULAR THERAPY-NUCLEIC ACIDS,11,34-40. |
MLA | Zheng, Haifeng,et al."Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects".MOLECULAR THERAPY-NUCLEIC ACIDS 11(2018):34-40. |
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