Institutional Repository of Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
Phage Display-Derived Peptide-Based Dual-Modality Imaging Probe for Bladder Cancer Diagnosis and Resection Postinstillation: A Preclinical Study | |
Peng, Li1,2,3; Shang, Wenting2,4![]() ![]() ![]() | |
发表期刊 | MOLECULAR CANCER THERAPEUTICS
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ISSN | 1535-7163 |
2018-10-01 | |
卷号 | 17期号:10页码:2100-2111 |
摘要 | Bladder cancer is a common human malignancy. Conventional ultrasound and white-light cystoscopy are often used for bladder cancer diagnosis and resection, but insufficient specificity results in a high bladder cancer recurrence rate. New strategies for the diagnosis and resection of bladder cancer are needed. In this study, we developed a highly specific peptide-based probe for bladder cancer photoacoustic imaging (PAI) diagnosis and near-infrared (NIR)-imaging-guided resection after instillation. A bladder cancer-specific peptide (PLSWT7) was selected by in vivo phage-display technology and labeled with IRDye800CW to synthesize a bladder cancer-specific dual-modality imaging (DMI) probe (PLSWT7-DMI). The feasibility of PLSWT7-DMI-based dual-modality PAI-NIR imaging was assessed in vitro, in mouse models, and ex vivo human bladders. An air-pouch bladder cancer (APBC) model suitable for probe instillation was established to evaluate the probe-based bladder cancer PAI diagnosis and NIR-imaging-guided resection. Human bladders were used to assess whether the PLSWT7-DMI-based DMI strategy is a translatable approach for bladder cancer detection and resection. The probe exhibited excellent selectivity and specificity both in vitro and in vivo. Postinstillation of the probe, tumors <3 mm were detectable by PAI, and NIR-imaging-guided tumor resection decreased the bladder cancer recurrence rate by 90% and increased the survival in the mouse model. Additionally, ex vivo NIR imaging of human bladders indicated that PLSWT7-DMI-based imaging would potentially allow precise resection of bladder cancer in clinical settings. This PLSWT7-DMI-based DMI strategy was a translatable approach for bladder cancer diagnosis and resection and could potentially lower the bladder cancer recurrence rate. (C) 2018 AACR. |
关键词 | Bladder Cancer |
DOI | 10.1158/1535-7163.MCT-18-0212 |
关键词[WOS] | IN-VIVO ; CYSTOSCOPY ; AGENTS ; URINE |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Natural Science Foundation of Heilongjiang Province of China[QC2017097] ; National Natural Science Foundation of China[81270022] ; National Natural Science Foundation of China[81501540] ; National Natural Science Foundation of China[61671449] ; National Key R&D Program of China[2017YFA0205200] ; Strategic Priority Research Program from Chinese Academy of Sciences[XDB02060010] ; Beijing Municipal Science & Technology Commission[Z161100002616022] ; National Natural Science Foundation of China[61231004] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[61231004] ; Beijing Municipal Science & Technology Commission[Z161100002616022] ; Strategic Priority Research Program from Chinese Academy of Sciences[XDB02060010] ; National Key R&D Program of China[2017YFA0205200] ; National Natural Science Foundation of China[61671449] ; National Natural Science Foundation of China[81501540] ; National Natural Science Foundation of China[81270022] ; Natural Science Foundation of Heilongjiang Province of China[QC2017097] |
WOS研究方向 | Oncology |
WOS类目 | Oncology |
WOS记录号 | WOS:000456145700003 |
出版者 | AMER ASSOC CANCER RESEARCH |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ia.ac.cn/handle/173211/25322 |
专题 | 中国科学院分子影像重点实验室 |
通讯作者 | Tian, Jie; Wang, Kun; Xu, Wanhai |
作者单位 | 1.Harbin Med Univ, Hosp 4, Urol Surg Dept, Harbin, Heilongjiang, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Mol Imaging, State Key Lab Management & Control Complex Syst, Inst Automat, Beijing, Peoples R China 3.Heilongjiang Key Lab Sci Res Urol, Harbin, Heilongjiang, Peoples R China 4.Chinese Acad Sci, Inst Automat, State Key Lab Management & Control Complex Syst, Beijing, Peoples R China 5.Beihang Univ, Beijing, Peoples R China |
通讯作者单位 | 中国科学院自动化研究所 |
推荐引用方式 GB/T 7714 | Peng, Li,Shang, Wenting,Guo, Pengyu,et al. Phage Display-Derived Peptide-Based Dual-Modality Imaging Probe for Bladder Cancer Diagnosis and Resection Postinstillation: A Preclinical Study[J]. MOLECULAR CANCER THERAPEUTICS,2018,17(10):2100-2111. |
APA | Peng, Li.,Shang, Wenting.,Guo, Pengyu.,He, Kunshan.,Wang, Hongzhi.,...&Xu, Wanhai.(2018).Phage Display-Derived Peptide-Based Dual-Modality Imaging Probe for Bladder Cancer Diagnosis and Resection Postinstillation: A Preclinical Study.MOLECULAR CANCER THERAPEUTICS,17(10),2100-2111. |
MLA | Peng, Li,et al."Phage Display-Derived Peptide-Based Dual-Modality Imaging Probe for Bladder Cancer Diagnosis and Resection Postinstillation: A Preclinical Study".MOLECULAR CANCER THERAPEUTICS 17.10(2018):2100-2111. |
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