Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects

doi:10.1021/acs.analchem.9b02935

	Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects
	Zheng, Haifeng 1,2; Chen, Si 1; Wang, Xinan 1; Xie, Jinrong 1; Tian, Jie 1,3,4; Wang, Fu 1,2
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
	2019-10-01
卷号	91 期号:19 页码:12392-12398
通讯作者	Tian, Jie(jie.tian@ia.ac.cn) ; Wang, Fu(fwang@xidian.edu.cn)
摘要	Pre-mRNA splicing in the information exchange from DNA to protein is a critical step in all eukaryotes. However, there is currently a lack of noninvasive approaches for monitoring mRNA splicing events in cells. In this study, we presented a genetically encoded bioluminescence reporter, Rluc-intron, for noninvasive real-time monitoring the premRNA splicing process in living cells and animals. It was designed by inserting a renilla luciferase (Rluc) gene into an intron sequence manipulated by RNA splicing modulator. We demonstrated that the splicing reporter Rluc-intron could provide real-time and quantitative information on the splicing activity responded to extracellular stimuli in living cells. In addition, Rluc-intron reporter is able to successfully quantify and image the pre-mRNA splicing in living mice in a noninvasive and longitudinal manner. This bioluminescence reporter provides the advantageous properties of systematic discovery of splicing modulators, which give the advances in pharmacogenomics and would produce new approach in the therapy of human diseases caused by splicing disorder.
DOI	10.1021/acs.analchem.9b02935
关键词[WOS]	ANTISENSE OLIGONUCLEOTIDES ; RETENTION
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81571721] ; National Natural Science Foundation of China[81772010] ; National Key Research and Development Program of China (973 Program)[2017YFA0205202] ; National Natural Science Foundation of China[81571721] ; National Natural Science Foundation of China[81772010] ; National Key Research and Development Program of China (973 Program)[2017YFA0205202]
项目资助者	National Natural Science Foundation of China ; National Key Research and Development Program of China (973 Program)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000488993000036
出版者	AMER CHEMICAL SOC
七大方向——子方向分类	医学影像处理与分析
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/26445
专题	中国科学院分子影像重点实验室
通讯作者	Tian, Jie; Wang, Fu
作者单位	1.Xidian Univ, Sch Life Sci & Technol, Minist Educ, Engn Res Ctr Mol & Neuro Imaging, Xian 710071, Shaanxi, Peoples R China 2.Northwestern Polytech Univ, Inst Med Res, Xian 710072, Shaanxi, Peoples R China 3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China 4.Beihang Univ, Sch Med, Beijing Adv Innovat Ctr Big Data Based Precis Med, Beijing 100190, Peoples R China
通讯作者单位	中国科学院分子影像重点实验室
推荐引用方式 GB/T 7714	Zheng, Haifeng,Chen, Si,Wang, Xinan,et al. Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects[J]. ANALYTICAL CHEMISTRY,2019,91(19):12392-12398.
APA	Zheng, Haifeng,Chen, Si,Wang, Xinan,Xie, Jinrong,Tian, Jie,&Wang, Fu.(2019).Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects.ANALYTICAL CHEMISTRY,91(19),12392-12398.
MLA	Zheng, Haifeng,et al."Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects".ANALYTICAL CHEMISTRY 91.19(2019):12392-12398.